| Successful modification | The parasite was generated by the genetic modification |
| The mutant contains the following genetic modification(s) |
Gene disruption
|
| Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 23490234
|
| MR4 number |
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| Parent parasite used to introduce the genetic modification |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | P. berghei ANKA |
| Name parent line/clone |
P. berghei ANKA cl15cy1
|
| Other information parent line | A reference wild type clone from the ANKA strain of P. berghei (PubMed: PMID: 17406255). |
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| The mutant parasite was generated by |
| Name PI/Researcher | J. Lin, G.R. Mair, C.J. Janse, S.M. Khan |
| Name Group/Department | Leiden Malaria Research Group |
| Name Institute | Leiden University Medical Center (LUMC) |
| City | Leiden |
| Country | The Netherlands |
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| Name of the mutant parasite |
| RMgm number | RMgm-178 |
| Principal name | 430cl1; 687cl1; 687cl2 |
| Alternative name | ∆rom3 |
| Standardized name | |
| Is the mutant parasite cloned after genetic modification | Yes |
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| Phenotype |
| Asexual blood stage | Not different from wild type |
| Gametocyte/Gamete | Not different from wild type |
| Fertilization and ookinete | Not different from wild type |
| Oocyst | Normal numbers of oocysts are produced. Mature (11-13 day) mutant oocysts have the same size as wild type oocysts. No sporozoites are formed within the oocysts. Oocysts appear vacuolated. |
| Sporozoite | Normal numbers of oocysts are produced. Mature (11-13 day) mutant oocysts have the same size as wild type oocysts. No sporozoites are formed within the oocysts. Oocysts appear vacuolated. |
| Liver stage | Not tested |
| Additional remarks phenotype | Mutant/mutation
The mutant lacks expression of rhomboid protease ROM3.
Protein (function)
Rhomboid proteins are intra-membrane proteases that play a role in multiple processes. They belong to a family of serine proteases that cleave cell-surface proteins within their transmembrane domains. The Plasmodium genome encodes a total of 8 rhomboid proteases (ROM1, 3, 4, 6, 7, 8, 9 and 10). .
Phenotype
The phenotype analyses indicate that ROM3 plays an essential role in oocyst development and in the formation of sporozoites. Sporozoite formation is completely blocked in the absence of ROM3.
Additional information
Transcripts of rom3 were not detected in asexual blood stage parasites. Relatively high transcription levels were observed in the gametocyte and ookinete stage. See also RMgm-763 for a P. berghei mutant expressing a (C-terminal) GFP-tagged version of rhomboid protease ROM3. ROM3-GFP expression was observed in female gametocytes and ookinetes while no GFP-signals were detected in developing oocysts.
Other mutants
RMgm-176: A P. berghei mutant lacking expression of rhomboid protease ROM1 (partial disruption)
RMgm-177: A P. berghei mutant lacking expression of rhomboid protease ROM1 (partial disruption)
RMgm-761: A P. berghei mutant lacking expression of rhomboid protease ROM1 (complete disruption)
RMgm-762: A P. berghei mutant lacking expression of rhomboid protease ROM9
RMgm-179: A P. berghei mutant lacking expression of rhomboid protease ROM10
RMgm-187: Unsuccessful attempts to disrupt P. berghei rhomboid protease rom4
RMgm-758: Unsuccessful attempts to disrupt P. berghei rhomboid protease rom6
RMgm-759: Unsuccessful attempts to disrupt P. berghei rhomboid protease rom7
RMgm-760: Unsuccessful attempts to disrupt P. berghei rhomboid protease rom8
RMgm-763: A P. berghei mutant expressing a (C-terminal) GFP-tagged version of rhomboid protease ROM3
RMgm-764: A P. berghei mutant expressing a (C-terminal) mCherry-tagged version of rhomboid protease ROM4
RMgm-659: A P. y. yoelii 17XNL mutant lacking expression of rhomboid protease ROM1
RMgm-660: A P. y. yoelii 17XNL mutant expressing a (N-terminal) HA-tagged version of rhomboid protease ROM1 |
*RMgm-178
