| Successful modification | The parasite was generated by the genetic modification |
| The mutant contains the following genetic modification(s) |
Gene disruption
|
| Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 21819513
|
| MR4 number |
|
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| Parent parasite used to introduce the genetic modification |
| Rodent Malaria Parasite | P. yoelii |
| Parent strain/line | P. y. yoelii 17XNL |
| Name parent line/clone |
Not applicable
|
| Other information parent line | 17XNL is a non-lethal strain of P. yoelii |
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| The mutant parasite was generated by |
| Name PI/Researcher | D.C. MacKellar; S.H.I. Kappe |
| Name Group/Department | Molecular and Cellular Biology Program |
| Name Institute | University of Washington |
| City | Seattle, Washington |
| Country | USA |
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| Name of the mutant parasite |
| RMgm number | RMgm-648 |
| Principal name | PY03365- |
| Alternative name | |
| Standardized name | |
| Is the mutant parasite cloned after genetic modification | No |
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| Phenotype |
| Asexual blood stage | Not different from wild type |
| Gametocyte/Gamete | Not different from wild type |
| Fertilization and ookinete | Not different from wild type |
| Oocyst | Not different from wild type |
| Sporozoite | Not different from wild type |
| Liver stage | Not different from wild type |
| Additional remarks phenotype | Mutant/mutation
The mutant lacks expression of PY03365.
The mutant was not cloned and analyzed as an enriched population of transgenic parasites containing residual P. yoelii wild type parasites.
Protein (function)
Early transcribed membrane protein (ETRAMP) family member. Plasmodium conserved family with greater than ten members in P. falciparum. ETRAMPs are abundantly expressed early in the intraerythrocytic cycle and are small (frequently less than 200 aa) integral membrane proteins that are localized within the parasitophorous vacuolar membrane (PVM). All members have signal peptides plus a transmembrane domain. The ETRAMP/SEP proteins of P. yoelii and P. berghei (8-11 genes) show homology to members of the ETRAMP family of proteins of P. falciparum (14 genes) but the orthologous relationship of the different members is not completely resolved.
Phenotype
Phenotype analyses indicate that PY03365 has no essential role during the complete life cycle. However, phenotypes of the uncloned mutants has not been analysed in great detail and therefore minor effects of the lack of expression of PY00205 on blood stage, mosquito stage and liver stage development cannot be ruled out..
Additional information
The ETRAMP/SEP proteins of P. yoelii and P. berghei (8-11 genes) show homology to members of the ETRAMP family of proteins of P. falciparum (14 genes) but the orthologous relationship of the different members is not completely resolved.
| P. falciparum |
P. yoelii |
P. berghei |
Syntenic |
|
PFB0120w (ETRAMP2)
PF11_0040 (ETRAMP11.2)
|
PY00205 |
PBANKA_050110 (PbSEP3) |
Py-Pb |
| PFD1120c (ETRAMP4) |
PY03869 |
PBANKA_052420 (PbSEP2) |
Py-Pb |
| PF10_0323 (ETRAP10.2) |
PY07506 |
PBANKA_051700 |
Py-Pb |
| PF10_0164 (ETRAMP10.3) |
PY00204 (PyUIS4) |
PBANKA_050120 (UIS4) |
Py-Pb |
| PF11_0039 (ETRAMP11.1) |
PY04799 |
PBANKA_052480 (PbSEP1) |
|
| PF13_0012 (ETRAMP13) |
PY03011 (PyUIS3) |
PBANKA_140080(UIS3) |
Py-Pb |
| - |
Py02667 |
PBANKA_020160 |
Py-Pb |
| - |
PY03365
PY05433
PY06488 |
- |
|
| - |
PY03652 |
- |
|
Other mutants
RMgm-641: A mutant expressing a second, c-myc tagged copy of PY03652 under control of the endogenous promoter
RMgm-640: mutant expressing a c-myc tagged version of PY02667 under control of the endogenous promoter
RMgm-643: A mutant lacking expression of PY03652. The mutant was cloned and the entire life cycle analyzed
RMgm-644: A mutant lacking expression of PY04799. The mutant was cloned and the entire life cycle analyzed
RMgm-646: A mutant lacking expression of PY00205. The mutant was analyzed as an enriched population of transgenic parasites containing residual P. yoelii WT
RMgm-647: A mutant lacking expression of PY03869. The mutant was analyzed as an enriched population of transgenic parasites containing residual P. yoelii WT
RMgm-649: A mutant lacking expression of PY05433. The mutant was analyzed as an enriched population of transgenic parasites containing residual P. yoelii WT
RMgm-650: A mutant lacking expression of PY06488. The mutant was analyzed as an enriched population of transgenic parasites containing residual P. yoelii WT
RMgm-642: unsuccessful attempt to disrupt PY02667
RMgm-645: unsuccessful attempt to disrupt PY07506
|
*RMgm-648
