Successful modification | The parasite was generated by the genetic modification |
The mutant contains the following genetic modification(s) |
Gene tagging,
Introduction of a transgene
|
Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 29404413 |
MR4 number |
|
top of page |
Parent parasite used to introduce the genetic modification |
Rodent Malaria Parasite | P. berghei |
Parent strain/line | P. berghei ANKA |
Name parent line/clone |
RMgm-928
|
Other information parent line | A P. berghei reference line expressing mCherry under the control of the P. berghei hsp70 promoter. The mutant does not contain a drug-selectable marker. The mCherry expression cassette has been introduced into the silent p23p locus |
top of page |
The mutant parasite was generated by |
Name PI/Researcher | Schnider CB, Burda PC |
Name Group/Department | Institute of Cell Biology |
Name Institute | University of Bern |
City | Bern |
Country | Switzerland |
top of page |
Name of the mutant parasite |
RMgm number | RMgm-4421 |
Principal name | PBANKA_0519400-GFP |
Alternative name | |
Standardized name | |
Is the mutant parasite cloned after genetic modification | Yes |
top of page |
Phenotype |
Asexual blood stage | PBANKA_0519400 was found mainly in the PV or PPM during blood and liver stage development. PBANKA_0519400 surrounded developing blood stage merozoites individually, while no GFP signal was found around free merozoites, pointing to PV localization of this protein during blood stage development. Similarly, in liver stage cytomeres, the protein did not colocalize with EXP1 and instead showed a typical PV/PPM localization. To investigate this further, we performed colocalization analysis with the PPM marker protein merozoite surface protein 1 (MSP1). PBANKA_0519400 colocalized with MSP1 only partially and concentrated at the parasite periphery of late liver stage parasites, which is consistent with PV localization also during liver stage development. |
Gametocyte/Gamete | Not tested |
Fertilization and ookinete | Not tested |
Oocyst | Not tested |
Sporozoite | Not tested |
Liver stage | PBANKA_0519400 was found mainly in the PV or PPM during blood and liver stage development. PBANKA_0519400 surrounded developing blood stage merozoites individually, while no GFP signal was found around free merozoites, pointing to PV localization of this protein during blood stage development. Similarly, in liver stage cytomeres, the protein did not colocalize with EXP1 and instead showed a typical PV/PPM localization. To investigate this further, we performed colocalization analysis with the PPM marker protein merozoite surface protein 1 (MSP1). PBANKA_0519400 colocalized with MSP1 only partially and concentrated at the parasite periphery of late liver stage parasites, which is consistent with PV localization also during liver stage development. |
Additional remarks phenotype | Mutant/mutation
The mutant expresses a C-terminal GFP tagged version of PBANKA_0519400.
In addition it expresses mCherry under the hsp70 promoter.
Protein (function)
The protein was selected in a screen of parasitophorous vacuole membrane (PVM) proteins using a BioID approach (see mutant RMgm-4417).
A list of 61 known and candidate PVM proteins were identified. Seven proteins were analyzed further during blood and liver stage development. This resulted in the identification of three novel PVM proteins, which were the serine/threonine protein phosphatase UIS2 (PlasmoDB accession no. PBANKA_1328000) and two conserved Plasmodium proteins with unknown functions (PBANKA_0519300 and PBANKA_0509000).
Phenotype
PBANKA_0519400 was found mainly in the PV or PPM during blood and liver stage development. PBANKA_0519400 surrounded developing blood stage merozoites individually, while no GFP signal was found around free merozoites, pointing to PV localization of this protein during blood stage development. Similarly, in liver stage cytomeres, the protein did not colocalize with EXP1 and instead showed a typical PV/PPM localization. To investigate this further, we performed colocalization analysis with the PPM marker protein merozoite surface protein 1 (MSP1). PBANKA_0519400 colocalized with MSP1 only partially and concentrated at the parasite periphery of late liver stage parasites, which is consistent with PV localization also during liver stage development.
Additional information
Other mutants |