Summary

RMgm-704
Malaria parasiteP. berghei
Genotype
TaggedGene model (rodent): PBANKA_0100600; Gene model (P.falciparum): Not available; Gene product: schizont membrane associated cytoadherence protein (SMAC: Schizont Membrane Associated Cytoadherence protein)
Name tag: mCherry
Phenotype Asexual bloodstage;
Last modified: 2 December 2012, 15:31
  *RMgm-704
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene tagging
Reference (PubMed-PMID number) Reference 1 (PMID number) : 23197789
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei K173
Name parent line/clone P. berghei K173 cl1
Other information parent lineThe K173cl1 is a cloned laboratory line of the K173 strain of P. berghei that lacks gametocyte production and sequestration of schizonts. The absence gametocyte production and schizont-sequestration can most likely be explained by the laboratory history of this line; it has been kept for more than 20 years in mice by mechanical blood passage; its chromosomes are reduced in size as a result of the loss of subtelomeric genes and repeat elements (PubMed: PMID: 2674047; 2649389). This line has frequently been used to study P. berghei ECM (PubMed: PMID: 2230643; 9806067).
The mutant parasite was generated by
Name PI/ResearcherE.M. Pasini, C.J. Janse, B.M.D. Franke-Fayard
Name Group/DepartmentDepartment of Parasitology
Name InstituteBiomedical Primate Research Centre
CityRijswijk
CountryThe Netherlands
Name of the mutant parasite
RMgm numberRMgm-704
Principal name1276cl1
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationYes
Phenotype
Asexual blood stageThe tagged-protein is exported into the cytoplasm of the host erythrocyte and shows a diffuse or patchy localization in the cytoplasm
Gametocyte/GameteNot tested
Fertilization and ookineteNot tested
OocystNot tested
SporozoiteNot tested
Liver stageNot tested
Additional remarks phenotype

Mutant/mutation

Protein (function)

Phenotype

Additional information
The mutant parasite expresses an mCherry-tagged protein. The protein has been selected for tagging in a screen for putative exported proteins of P. berghei.

The genotype of the parasites has not been analysed in detail.
Southern analysis of PFG-separated chromosomes (to show integration into the chromosome on which the target gene is located) has been performed (see below). This analysis provided evidence for tagging of the correct gene. The construct used aims at integration of the tagging construct by single-cross-over integration resulting in the presence of a tagged copy of the endogenous gene.

Phenotype analyses of the blood stages by fluorescence microscopy show that the tagged-protein is exported into the cytoplasm of the host erythrocyte and shows a diffuse or patchy localization in the cytoplasm.




Other mutants
RMgm-663: P. berghei ANKA cl15cy1 mutant expressing a (C-terminal) mCherry-tagged version of Schizont Membrane Associated Cytoadherence protein (SMAC).


  Tagged: Mutant parasite with a tagged gene
Details of the target gene
Gene Model of Rodent Parasite PBANKA_0100600
Gene Model P. falciparum ortholog Not available
Gene productschizont membrane associated cytoadherence protein
Gene product: Alternative nameSMAC: Schizont Membrane Associated Cytoadherence protein
Details of the genetic modification
Name of the tagmCherry
Details of taggingC-terminal
Additional remarks: tagging
Commercial source of tag-antibodiesClontech
Type of plasmid/constructPlasmid single cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
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Plasmid/construct sequence
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CTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTC
ATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGA
GATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTC
CAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACC
CTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAG
CCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAA
AGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCAC
CACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCATTCAGGCTGCG
CAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGG
GGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTG
TAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAATTGGAGCTCCA
CCGCGGTGGCGGCCGCTCTAGAACTAGTGTTACATTTATATATTGAACCCATTTTAAAAA
TATACATATTAAATAGCTTTAGAATTATGACAATTAATTTTTATAATAATAAAAAGTTCC
ATTTATTTGTTTTCTGAATCGGTAAAGAGTATTAATAAAAACCCAATTGTAAACGGTCCC
AATATATTTTTTAAAGAATGTGTTACTATGTATTTAAATTACCCAAATTAAACAAGTAAA
AAATATCACAATATATATTATTTTCCCCGAGATGCTATAAATAATTATTTTTGATAGATT
GACAAATATATATTGTTCTTTTTTGACGTAATGCATTATATATCATATATGTAGCACTTT
TTAGTTTTAAAATAATAATTTAATATAATACACAGTGCATGCACCATTTTACATGAATCT
GATATAATACTTATTTATCATTTTATTTATTAGCCATTTATTATAAACAAACTATATATT
ATTATCTTTTACATACATCCTTAACTCTTTTAACAACCAGTACACATAATATTTGCAACA
TTGTGAATATAAAATGAAATATTTAACGCACATATTTTTTTTTATTATACTTTTTCAATT
TATAGTTGATAAATACGATAACAATGGAAAATCATTGGACAAGTTTTTAAATATACGAAA
TGAGAGATTTCTAGTAGAATATTATGACGCTAACATTGATCAATATAATGGCAATCAATC
AAAAAAAAAAAAAAATATATATACATATGAATCAAATGGTATTTCACAAAACACTACTAA
TACCAAAAACCAATCAGCAAGTTTTTATGCACAACCTACCCACGGCCAATATAAATACCA
TCGCAATAATGTATATACTCCCTCTGAAGAGTTCAATTTTTATAATAGTACGTTCATGGA
AAGGCTAAGACATAATTCTAAGATCATTATGGGATCATCTGCAGCGATATTTTTTCTTGT
CGAAGATTTAGGAATTAGAGCTGTGCTATTTTTAATATTTTCTGCTGCTGTACTCGCTTA
TTTCACTTCCATAGGATCCGGTGGAGGTGGAAGATCCATGGTGAGCAAGGGCGAGGAGGA
TAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAA
CGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGAC
CGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCC
TCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTT
GAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGG
CGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAA
GCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTG
GGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCA
GAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGC
CAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTC
CCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCAC
CGGCGGCATGGACGAGCTGTACAAGTAATCTAGAATTATTAATATATATGAATATATATA
CATCGTTGTATGCGTAATATACGTGTAACTGTTTTTTTTAATTTTTATATTAACTTTTCC
TTATACATTTTAATGCCATTAACTAATAATATTTTACTTGAAAAAATTCTAAATTTTTAT
AACTCTAATATAAAATCTGTGAATAATAAAAAATTAGCATAAAGTACGAATTAAACATCG
AATATTTGTTTTAAAGGGGCACACATTATGTGCATATAATTTTATTCTTTGAATTTAATT
TTTTGCTTATAATTAGATTAAATTAATATAATTTTTTTATTGAAAATAAAAACTTATTTT
AAAAAATTAACAAATGGAAGTTAATGAAAAATACGAAAAAAGTAAAGGGTTAATTCTTAT
ATGGTCGGTACCGAGCTCGAATTCGATATCGAAATTGAAGGAAAAAACATCATTTGTGTT
TATATGTTTTTTTTTAATTTTTCAACTTTTTTTATTTAACTATGCTAATCATATTATAAA
CATTTTAAAAATCAAGTCCAACTATTTATGAATCATTGAAGAGACAACATAATTATAACA
GTAAAAATAATAATAATAATAATAATTTTGTCTTATTGTTTCAAACATATTATGTTCCAT
AAATATCTAAATTTTTAATATGGTCATAAATTCATAGGATTTAATTTATATAAAATATGG
CTATTCATACTAGCCATTTTATGTGTGTAAAGTTTTTTTTTTCTGAACAAAAAATAAAAT
GTTCATAATTTTAGCTATTTACATGCATGTGCATGCACAAAAAAAAATATGCACACAACA
TACACATTTTTACAGTTATAAATACAATCAATTGGTATAAATATATAAGTAAGAAAAACG
GGATCAAAGCTAGCTTCTGTATTTCCGCTAGACAGCCATCTCCATCTGGATTCGTCCGTG
CGGGACGTAGCCCACGACCTCAAAATCCTCGGCGGTGAAATCGTCGATTTCCTTGATGCG
TTCCTTGTTGAGGATGTTCACAATGGGGAACGGTCTCGGTTCTCTCCGCAGCTGCTCTTT
TAAAGCCTCGACATGGTTCGTGTAGACATGCGTGTTCCCCATGAAGTGAATGAACTCCTT
AGGTTTTAGGTTGCAGACGTGTGCAACCATGAGCGTCAAAAGCGAATAGGAAGCGATGTT
GAAGGGGACGCCGAGGCCGACATCGCACGACCGCTGATACATGATGCACGACAGCTCCTT
CTGGTCGTTCACGTAGAACTGGCACAACAAGTGACAAGGCGGCAGCGCCATTTCGTCCAG
CGCTGCAGGATTCCAGGCAGTCATGAGCATGCGACGATCTGTTGGATTCGTTCTCAGCAT
CTGGATCACATTCTTCAGCTGGTCGACGCCCTGCCCTGTGTAGTCTGTGTGCATGTCTTT
GTATGCCGCGCCGAAGTGTCTCCACTGGAAGCCGTAGCCCGGGCCGATGTCTCCGACCTC
TCGGTGGGGGAGATTGCGCGAATCGAGGAACTCGCGTGTCACATTCTTGTCCCAGATCTT
CACGCCCTTCTCAGAAAGATGGTTTGCGTTCGTGTCGCCGCGAATGAACCACAGCAACTC
TTCGAGGACCCCTTTCCAGAACACACGCTTTGTGGTGAGAAGTGGAAAGGCCTGATCCAG
CGAGTAGCGCATAGTGCAGCCGAATTTGGAGATGACACCAACGCCCGTTCGGTCATCCAT
TGTCCTTCCATTGTTAATAATGTCGGCAATGAGATCAAGGTACTGGAACTCTTCATGGCC
TCTAAAGTGAACATGCGGAACGGCCCGAATCAGTTCCTTTTGCTCGCGTTTTTTCCGGTC
TTCTTCGTCCATCCACGCCAACACCGGGGCAATGGCTGCGGCCGAAGAAGGAGCCTGCAA
CCCGTGCACGGGAGTTGTCTCCCTCGTGGACGTCAAGGAGCTCATTGCGTTGCTCGGTTC
CGCAGTGGCTGCGTCGTCAGTCTTCCTTCTCTTCTCGAGAACCACAAAGTCGTAGGGAAC
CCCGTTGTCTGAGAAGGTCTTGGAAATGAAGATGGGTCGATACGTCGCTTCATTGTCCTT
CTCTCTTCCGAGCTCCGGACAAAAGGGAACGAACACAGACTCGGCAGGAGCTGCAGCCTG
CGCAGCAGTTGATTTGTTTGAAAGAATGTCATCTCCGGGGAACGCAGGGAAGAAAACGTC
GCACGGAAACTCGCGGGCTACACGCGTGATGTACAGGTGAGAGGCAACGCCCAGAGACAG
CGCTGCCTCGTACAGTCCCGCTCCTCCCACGACAAAAATCTGGTCGACAGAATCCTTGTA
CTCTTCCTCCAGAAGGCTGAGAGCTGCTGGGAGTGAAGCACAGACTCGGACGCGCTGCTG
GCCTTCAGCTTGAGGCTTCTCCGCCGCAATGTCTTCTTCTTTGAGGGAAGAGGAAACGAC
GATGTTCAATCTGTCCACGAGGGGTCTAAACTTTCGAGGCATGCTTTCCCAGGTTTTCCG
TCCCATGACAACGGCGTTGAATCTCTTGCCGACTGATGGAGAGGGAAGTCCAGAGTCGCC
CGTCTTTGCAAATTTCCTGGGAAGCCACCCGTTCAGGCGACTGGCTTCTTCGGGCGTCGT
TTTTGTCACACGAGAAAAGTGTTTGAAATCTGTGGTCAAGTGGGGCCACGGGAGGCCGTT
GTTGATGCCGATGCCCCTCTTGGGGGTCATCGCGACGACCAGACACACCGGTTTATGCAT
TGATCCTTTGTAACATTTAGGTGTGTATTTATATATATAAGCAATATACAAATGAAAATA
CTATTATAAAACGGAAACTCAAAAAATGTTGAAACAAATAGTCAATTTTTAAAATGAATG
TGTAAATTGTTTAAGTTTTATGACAAAAAAAGGAAAAGTGTATATAAAATAAAAATTGGA
AAAAATTTATATATTACATGCCATATAATACCATTTATTTACTATTTATATCTTAATATA
AAAAATTGTAGCAAAAATATATAATCGTATTTTATATATTTAATATTATATAACCCCTTA
AAAAAAAAAATTTAATTGAAAAAATTTTAATTTATGCACTTTTTTATTGTATTATATATA
TTCCGTTATTTTAATTACACATAAACTTAATAGAAAAATTAATTCGCTGCTAAATAAAAA
TTATAGGGCTATATATGATGGGATCATATTCTATCTTTCTCTTTTTCAAATYCTTTATAT
TTTAGAATACAAATCGCTTTAAACAATAAAAAAGAGGTATATCCTACCCGAATTGACACA
CACAATAAATAATAAATATATAAATAATAAAAATATAAATATATAAATTATAAATATATA
AATTATATATATAAATATATTAACATGTGTGTAATTTGTTAAAATTGATATTAAACAGCT
TTGCATGGGGAAAGAGTGGCTTTTATAAGCGAATCATATAATACAGTGTTAATTTTAATA
TATAAAATATAATTTCCTTAAATTGTTATTTAACATTTTTAATTGCCTACCCARGGTAAT
ATAATATCCAAATGACAATCAAAGATAAATGAAATACCGCTCCATTTTTCCTATATATCC
ATTTTCATTTTGTGTAAATGTTCATAAAATTTTATCATATACTCATAAAAAACAGGGGAA
AAATGAAAAAATGAACGTCCACCCCGTGTGAATATGCTCATTTTGTATACTATACATTTT
TTTTTATATAATTTATATTTTCCAAATAAAAAAAATGTTTGTACATGTATATATCAATAT
TGTGTTATGGCATGATGAAAAACAATTATTTTATTCGTTTTTAAATTTGTAGAATTTCCC
TAAATTATTAATGATTTCATATTATAATATATGTATCTATATATTTTTAATGTGTAATTC
AAAATGGCGAAAAAGAAACAAACCATACTTTATAAGCAACTCTCTTTGAGCACTTACCAT
AAAAAATTATACTACTAAATTCAAATTAAATAAAAAAATATATATTATAAAATTGGCTTT
AATTATTTATAAGCACTTGCTTTGTTTTACTCAAAAAGTATTGGCATAAGTATAAACATA
AAACCAATTTTTCACAAAATATATTATATGTCCATACAACTATATCCGAACYTTCAAAAC
ACATCCAAATTTCCATTTACCACATGCAAGCAAAAGGATATTAAATAAAAAAAAATTATT
TATATTTTCATGATCACTTAAATAATATTTAAAAAAAAAAGAGACCATATATAAAATGTA
TAATTTAAATATAGCTAACTTATTAAAAAAAATGAATATTGAGCATATATCCATAATTTA
AAACACTTCTATTTTATGTTATTTGATATATAAGTCGATAAATTATTGCATGTATGCATA
TAGACAGATTAATATACACCAAAATAATAAAAATTATATATAGTTCATATATTATTCTGA
ATAATAAATTAGCTTTCCTCTTAATTCACTTGGTATGTATATATATATATATATATATAT
ATATATATATATGGATAAATAAATATAAGGTCTATAACAGGAAAAAATGTGTATTAACTT
TTTTTTATAATTATTTTATATTTTTTCTCCATTTATTTAAATATGTTAAAATTATTTCCC
ATTTTATAAACAAAAATTAATCCATAAAATAGCAAAATAACAAAATGACAAAATAACAAA
AACAAAAAAACGCATTATATGAGTTCATTTTACACAATCCAAAGAAAAAGAAAATATGCT
TAAAATATTCATAACACACTTTTAAGCTATAAAAATATATATATTTATTTGTGTCTATAT
TACCAACTCAATTTAATAGATGTGTTATGTGATTAATTCATACACAAACATACAAAAATA
AACACCATTTTTGAAAAGATTAATTTGAAATCATATATGTTATTATTATATATTTTTATA
TCAATGAAATTTAAAAAAATAAAAGGGAAATCAATGTATTAAAAATAATTATATGTTATT
TTATTTCCACAATATTTATTTATTATTTATTGTTGACCTGCAGGCATGCAAGCTTATCGA
TACCGTCGACCTCGAGGGGGGGCCCGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAA
TTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCA
CAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAG
TGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGT
CGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGC
GCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGG
TATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAA
AGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGG
CGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGA
GGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCG
TGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGG
GAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTC
GCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCG
GTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCA
CTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGT
GGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAG
TTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCG
GTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATC
CTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTT
TGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTT
TTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCA
GTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCG
TCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATAC
CGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGG
CCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCC
GGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTA
CAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAAC
GATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTC
CTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCAC
TGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACT
CAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAA
TACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTT
CTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCA
CTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAA
AAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATAC
TCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCG
GATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCC
GAAAAGTGCCAC
Restriction sites to linearize plasmid NdeI
Selectable marker used to select the mutant parasitetgdhfr
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1gggACTAGTGTTACATTTATATATTGAACCC
Additional information primer 1L3774: smac mCherry tagging, F (SpeI)
Sequence Primer 2ggAGATCTTCCACCTCCACCGGATCCTATGGAAGTGAAATAAGCGAGTAC
Additional information primer 2L3775: smac mCherry tagging, R (BglII, BamHI)
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6