RMgmDB - Rodent Malaria genetically modified Parasites
SummaryRMgm-704
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Last modified: 2 December 2012, 15:31
*RMgm-704| Successful modification | The parasite was generated by the genetic modification |
| The mutant contains the following genetic modification(s) | Gene tagging |
| Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 23197789 |
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| Parent parasite used to introduce the genetic modification | |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | P. berghei K173 |
| Name parent line/clone | P. berghei K173 cl1 |
| Other information parent line | The K173cl1 is a cloned laboratory line of the K173 strain of P. berghei that lacks gametocyte production and sequestration of schizonts. The absence gametocyte production and schizont-sequestration can most likely be explained by the laboratory history of this line; it has been kept for more than 20 years in mice by mechanical blood passage; its chromosomes are reduced in size as a result of the loss of subtelomeric genes and repeat elements (PubMed: PMID: 2674047; 2649389). This line has frequently been used to study P. berghei ECM (PubMed: PMID: 2230643; 9806067). |
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| The mutant parasite was generated by | |
| Name PI/Researcher | E.M. Pasini, C.J. Janse, B.M.D. Franke-Fayard |
| Name Group/Department | Department of Parasitology |
| Name Institute | Biomedical Primate Research Centre |
| City | Rijswijk |
| Country | The Netherlands |
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| Name of the mutant parasite | |
| RMgm number | RMgm-704 |
| Principal name | 1276cl1 |
| Alternative name | |
| Standardized name | |
| Is the mutant parasite cloned after genetic modification | Yes |
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| Phenotype | |
| Asexual blood stage | The tagged-protein is exported into the cytoplasm of the host erythrocyte and shows a diffuse or patchy localization in the cytoplasm |
| Gametocyte/Gamete | Not tested |
| Fertilization and ookinete | Not tested |
| Oocyst | Not tested |
| Sporozoite | Not tested |
| Liver stage | Not tested |
| Additional remarks phenotype | Mutant/mutation |
Tagged: Mutant parasite with a tagged gene| top of page | |||||||||||||||||||||||||||
| Details of the target gene | |||||||||||||||||||||||||||
| Gene Model of Rodent Parasite | PBANKA_0100600 | ||||||||||||||||||||||||||
| Gene Model P. falciparum ortholog | Not available | ||||||||||||||||||||||||||
| Gene product | schizont membrane associated cytoadherence protein | ||||||||||||||||||||||||||
| Gene product: Alternative name | SMAC: Schizont Membrane Associated Cytoadherence protein | ||||||||||||||||||||||||||
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| Details of the genetic modification | |||||||||||||||||||||||||||
| Name of the tag | mCherry | ||||||||||||||||||||||||||
| Details of tagging | C-terminal | ||||||||||||||||||||||||||
| Additional remarks: tagging | |||||||||||||||||||||||||||
| Commercial source of tag-antibodies | Clontech | ||||||||||||||||||||||||||
| Type of plasmid/construct | Plasmid single cross-over | ||||||||||||||||||||||||||
| PlasmoGEM (Sanger) construct/vector used | No | ||||||||||||||||||||||||||
| Modified PlasmoGEM construct/vector used | No | ||||||||||||||||||||||||||
| Plasmid/construct map |
  
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| Plasmid/construct sequence |
CTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTC
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| Restriction sites to linearize plasmid | NdeI | ||||||||||||||||||||||||||
| Selectable marker used to select the mutant parasite | tgdhfr | ||||||||||||||||||||||||||
| Promoter of the selectable marker | pbdhfr | ||||||||||||||||||||||||||
| Selection (positive) procedure | pyrimethamine | ||||||||||||||||||||||||||
| Selection (negative) procedure | No | ||||||||||||||||||||||||||
| Additional remarks genetic modification | |||||||||||||||||||||||||||
| Additional remarks selection procedure | |||||||||||||||||||||||||||
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