RMgmDB - Rodent Malaria genetically modified Parasites

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Summary

RMgm-702
Malaria parasiteP. berghei
Genotype
TaggedGene model (rodent): PBANKA_0316800; Gene model (P.falciparum): Not available; Gene product: erythrocyte membrane associated protein 2 (EMAP2: erythrocyte memberane associated protein 2)
Name tag: mCherry
Phenotype Asexual bloodstage;
Last modified: 2 December 2012, 15:31
  *RMgm-702
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene tagging
Reference (PubMed-PMID number) Reference 1 (PMID number) : v
MR4 number
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Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei K173
Name parent line/clone P. berghei K173 cl1
Other information parent lineThe K173cl1 is a cloned laboratory line of the K173 strain of P. berghei that lacks gametocyte production and sequestration of schizonts. The absence gametocyte production and schizont-sequestration can most likely be explained by the laboratory history of this line; it has been kept for more than 20 years in mice by mechanical blood passage; its chromosomes are reduced in size as a result of the loss of subtelomeric genes and repeat elements (PubMed: PMID: 2674047; 2649389). This line has frequently been used to study P. berghei ECM (PubMed: PMID: 2230643; 9806067).
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The mutant parasite was generated by
Name PI/ResearcherE.M. Pasini, C.J. Janse, B.M.D. Franke-Fayard
Name Group/DepartmentDepartment of Parasitology
Name InstituteBiomedical Primate Research Centre
CityRijswijk
CountryThe Netherlands
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Name of the mutant parasite
RMgm numberRMgm-702
Principal name2065; 2066
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationNo
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Phenotype
Asexual blood stageThe tagged-protein is exported into the cytoplasm of the host erythrocyte and shows a location at the surface membrane of infected erythrocytes
Gametocyte/GameteNot tested
Fertilization and ookineteNot tested
OocystNot tested
SporozoiteNot tested
Liver stageNot tested
Additional remarks phenotype

Mutant/mutation

Protein (function)

Phenotype

Additional information
The mutant parasite expresses an mCherry-tagged protein. The protein has been selected for tagging in a screen for putative exported proteins of P. berghei.

The genotype of the parasites has not been analysed in detail.
Southern analysis of PFG-separated chromosomes (to show integration into the chromosome on which the target gene is located) has been performed (see below). This analysis provided evidence for tagging of the correct gene. The construct used aims at integration of the tagging construct by single-cross-over integration resulting in the presence of a tagged copy of the endogenous gene.

Phenotype analyses of the blood stages by fluorescence microscopy show that the tagged-protein is exported into the cytoplasm of the host erythrocyte and shows a location at the surface membrane of infected erythrocytes.



Other mutants
RMgm-701: mutant expressing an mCherry-tagged version of PBANKA_021550
RMgm-703: mutant lacking expression of PBANKA_010060 (SMAC) and expressing GFP-luciferase under control of a schizont-specific promoter (RMgm-662). This mutant is also expressing an mCherry-tagged version of PBANKA_021550
RMgm-715: mutant lacking expression of PBANKA_021550

  Tagged: Mutant parasite with a tagged gene
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Details of the target gene
Gene Model of Rodent Parasite PBANKA_0316800
Gene Model P. falciparum ortholog Not available
Gene producterythrocyte membrane associated protein 2
Gene product: Alternative nameEMAP2: erythrocyte memberane associated protein 2
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Details of the genetic modification
Name of the tagmCherry
Details of taggingC-terminal
Additional remarks: tagging
Commercial source of tag-antibodiesClontech
Type of plasmid/constructPlasmid single cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
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Plasmid/construct sequence
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CTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTC
ATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGA
GATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTC
CAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACC
CTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAG
CCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAA
AGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCAC
CACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCATTCAGGCTGCG
CAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGG
GGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTG
TAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAATTGGAGCTCCA
CCGCGGTGGCGGCCGCTCTAGAACTAGTATATTATTTTATATATAAAACATCTCAATATT
TTTTTTTCTTTGAATTTCTTATATTATTAAAAAAAATATAATATACACTGTAAAAATGAA
TTACAAATTAATACAAATTACTTCGTCTCTTCTTTTAATGTGCTTATGTGAAGCATACAG
TAACGAGGTAAAATTCTATATATTAAATTTATAAATATTTATTTATTGTGCTAGAATCTA
TAATATGATAATATTTTACATTATAAAATAAAATTTAATTCTACATATATTCAAAATTTA
TGTATTTTTAGAAAAATAATAATAATGGGACTATATTTACCAAATCAAATCGATTGTTAT
CTGAGCCAGAAAACAATGAAATGGGAGAAGGATTATATGAAGAAAATGTATCACTATATG
ATGACAAAGAGCCTATAAGCGGCGACCAAGAATCAATTTTTGAAGATCAAACAAATAATG
AAGATCAACCAATTTTTGAAGATCAAACAAATAATGAAGATCAACCAATTTTTGAAGATC
AAACAAATAATGAAGATCAACCAATTTTTGAAGATCAAACAAATAATGAAGATCAACCAA
TTTTTGAAGATCAACCAATTTTTGAAGATCAACCAATTTTTGAAGATCAACCAATTTTTG
AAGATCAACCAATTTTTGAAGATCAACCAATTTTTGAAGATCAAACAAATAATGATGATC
AAACAAATAATGAAGATCAAACAAATAATGAAGATCAACCAATTTTTGAAGATCAACCAA
TTTTTGAAGATCAAACAAATAATGAAGATCAACCAATTTTTGAAGATCAAACAAATAATG
AAGATCAACCAATTTTTGAAGATCAAACAAATAATGAAGATCAAACAAATAATGAAGATC
AACTAATTTTTGAAGATCAAACAAATAATGAAGATCAACCAATTTTTGAAGATCAAACAA
ATAATGAAGATCAACCAATTTTTGAAGATCAAACAAATAATGATGATCAAACAAATAATG
AAGATCAACCAAATAATGAACCAGTAATGAAAATTTATGTCCCAAAAGACCCACCTTTTG
CAAAAACAAAAATAAACACCACTCCAAAACCAAATATAAGAACAGGGATACCACCAGAGC
ACATACAAATTATAGAAATGCCATTTAGATCACCTACTCCTCCTCCACCACCAGTAAAGG
TGACCTACAAAACCTCAGTAATCCCAAAACTTAAATTAAAAAAACCATCTCAACCGGTGG
ATAATTCATGTGACATGTATCCAAACAGTAAAGAAATTGAAGATGATATGATCGAGTGGG
ATAACTTTATATATGTAATTGATAACGTTGATTCAGATGATCCAGGCTTAGAAGATTATT
TGAAAGATAAAGTTTATCCAGGATCCGGTGGAGGTGGAAGATCCATGGTGAGCAAGGGCG
AGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCT
CCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCA
CCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCC
TGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCG
ACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGG
ACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACA
AGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCA
TGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGA
TCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCT
ACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACA
TCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCC
ACTCCACCGGCGGCATGGACGAGCTGTACAAGTAATCTAGAATTATTAATATATATGAAT
ATATATACATCGTTGTATGCGTAATATACGTGTAACTGTTTTTTTTAATTTTTATATTAA
CTTTTCCTTATACATTTTAATGCCATTAACTAATAATATTTTACTTGAAAAAATTCTAAA
TTTTTATAACTCTAATATAAAATCTGTGAATAATAAAAAATTAGCATAAAGTACGAATTA
AACATCGAATATTTGTTTTAAAGGGGCACACATTATGTGCATATAATTTTATTCTTTGAA
TTTAATTTTTTGCTTATAATTAGATTAAATTAATATAATTTTTTTATTGAAAATAAAAAC
TTATTTTAAAAAATTAACAAATGGAAGTTAATGAAAAATACGAAAAAAGTAAAGGGTTAA
TTCTTATATGGTCGGTACCGAGCTCGAATTCGATATCGAAATTGAAGGAAAAAACATCAT
TTGTGTTTATATGTTTTTTTTTAATTTTTCAACTTTTTTTATTTAACTATGCTAATCATA
TTATAAACATTTTAAAAATCAAGTCCAACTATTTATGAATCATTGAAGAGACAACATAAT
TATAACAGTAAAAATAATAATAATAATAATAATTTTGTCTTATTGTTTCAAACATATTAT
GTTCCATAAATATCTAAATTTTTAATATGGTCATAAATTCATAGGATTTAATTTATATAA
AATATGGCTATTCATACTAGCCATTTTATGTGTGTAAAGTTTTTTTTTTCTGAACAAAAA
ATAAAATGTTCATAATTTTAGCTATTTACATGCATGTGCATGCACAAAAAAAAATATGCA
CACAACATACACATTTTTACAGTTATAAATACAATCAATTGGTATAAATATATAAGTAAG
AAAAACGGGATCAAAGCTAGCTTCTGTATTTCCGCTAGACAGCCATCTCCATCTGGATTC
GTCCGTGCGGGACGTAGCCCACGACCTCAAAATCCTCGGCGGTGAAATCGTCGATTTCCT
TGATGCGTTCCTTGTTGAGGATGTTCACAATGGGGAACGGTCTCGGTTCTCTCCGCAGCT
GCTCTTTTAAAGCCTCGACATGGTTCGTGTAGACATGCGTGTTCCCCATGAAGTGAATGA
ACTCCTTAGGTTTTAGGTTGCAGACGTGTGCAACCATGAGCGTCAAAAGCGAATAGGAAG
CGATGTTGAAGGGGACGCCGAGGCCGACATCGCACGACCGCTGATACATGATGCACGACA
GCTCCTTCTGGTCGTTCACGTAGAACTGGCACAACAAGTGACAAGGCGGCAGCGCCATTT
CGTCCAGCGCTGCAGGATTCCAGGCAGTCATGAGCATGCGACGATCTGTTGGATTCGTTC
TCAGCATCTGGATCACATTCTTCAGCTGGTCGACGCCCTGCCCTGTGTAGTCTGTGTGCA
TGTCTTTGTATGCCGCGCCGAAGTGTCTCCACTGGAAGCCGTAGCCCGGGCCGATGTCTC
CGACCTCTCGGTGGGGGAGATTGCGCGAATCGAGGAACTCGCGTGTCACATTCTTGTCCC
AGATCTTCACGCCCTTCTCAGAAAGATGGTTTGCGTTCGTGTCGCCGCGAATGAACCACA
GCAACTCTTCGAGGACCCCTTTCCAGAACACACGCTTTGTGGTGAGAAGTGGAAAGGCCT
GATCCAGCGAGTAGCGCATAGTGCAGCCGAATTTGGAGATGACACCAACGCCCGTTCGGT
CATCCATTGTCCTTCCATTGTTAATAATGTCGGCAATGAGATCAAGGTACTGGAACTCTT
CATGGCCTCTAAAGTGAACATGCGGAACGGCCCGAATCAGTTCCTTTTGCTCGCGTTTTT
TCCGGTCTTCTTCGTCCATCCACGCCAACACCGGGGCAATGGCTGCGGCCGAAGAAGGAG
CCTGCAACCCGTGCACGGGAGTTGTCTCCCTCGTGGACGTCAAGGAGCTCATTGCGTTGC
TCGGTTCCGCAGTGGCTGCGTCGTCAGTCTTCCTTCTCTTCTCGAGAACCACAAAGTCGT
AGGGAACCCCGTTGTCTGAGAAGGTCTTGGAAATGAAGATGGGTCGATACGTCGCTTCAT
TGTCCTTCTCTCTTCCGAGCTCCGGACAAAAGGGAACGAACACAGACTCGGCAGGAGCTG
CAGCCTGCGCAGCAGTTGATTTGTTTGAAAGAATGTCATCTCCGGGGAACGCAGGGAAGA
AAACGTCGCACGGAAACTCGCGGGCTACACGCGTGATGTACAGGTGAGAGGCAACGCCCA
GAGACAGCGCTGCCTCGTACAGTCCCGCTCCTCCCACGACAAAAATCTGGTCGACAGAAT
CCTTGTACTCTTCCTCCAGAAGGCTGAGAGCTGCTGGGAGTGAAGCACAGACTCGGACGC
GCTGCTGGCCTTCAGCTTGAGGCTTCTCCGCCGCAATGTCTTCTTCTTTGAGGGAAGAGG
AAACGACGATGTTCAATCTGTCCACGAGGGGTCTAAACTTTCGAGGCATGCTTTCCCAGG
TTTTCCGTCCCATGACAACGGCGTTGAATCTCTTGCCGACTGATGGAGAGGGAAGTCCAG
AGTCGCCCGTCTTTGCAAATTTCCTGGGAAGCCACCCGTTCAGGCGACTGGCTTCTTCGG
GCGTCGTTTTTGTCACACGAGAAAAGTGTTTGAAATCTGTGGTCAAGTGGGGCCACGGGA
GGCCGTTGTTGATGCCGATGCCCCTCTTGGGGGTCATCGCGACGACCAGACACACCGGTT
TATGCATTGATCCTTTGTAACATTTAGGTGTGTATTTATATATATAAGCAATATACAAAT
GAAAATACTATTATAAAACGGAAACTCAAAAAATGTTGAAACAAATAGTCAATTTTTAAA
ATGAATGTGTAAATTGTTTAAGTTTTATGACAAAAAAAGGAAAAGTGTATATAAAATAAA
AATTGGAAAAAATTTATATATTACATGCCATATAATACCATTTATTTACTATTTATATCT
TAATATAAAAAATTGTAGCAAAAATATATAATCGTATTTTATATATTTAATATTATATAA
CCCCTTAAAAAAAAAAATTTAATTGAAAAAATTTTAATTTATGCACTTTTTTATTGTATT
ATATATATTCCGTTATTTTAATTACACATAAACTTAATAGAAAAATTAATTCGCTGCTAA
ATAAAAATTATAGGGCTATATATGATGGGATCATATTCTATCTTTCTCTTTTTCAAATYC
TTTATATTTTAGAATACAAATCGCTTTAAACAATAAAAAAGAGGTATATCCTACCCGAAT
TGACACACACAATAAATAATAAATATATAAATAATAAAAATATAAATATATAAATTATAA
ATATATAAATTATATATATAAATATATTAACATGTGTGTAATTTGTTAAAATTGATATTA
AACAGCTTTGCATGGGGAAAGAGTGGCTTTTATAAGCGAATCATATAATACAGTGTTAAT
TTTAATATATAAAATATAATTTCCTTAAATTGTTATTTAACATTTTTAATTGCCTACCCA
RGGTAATATAATATCCAAATGACAATCAAAGATAAATGAAATACCGCTCCATTTTTCCTA
TATATCCATTTTCATTTTGTGTAAATGTTCATAAAATTTTATCATATACTCATAAAAAAC
AGGGGAAAAATGAAAAAATGAACGTCCACCCCGTGTGAATATGCTCATTTTGTATACTAT
ACATTTTTTTTTATATAATTTATATTTTCCAAATAAAAAAAATGTTTGTACATGTATATA
TCAATATTGTGTTATGGCATGATGAAAAACAATTATTTTATTCGTTTTTAAATTTGTAGA
ATTTCCCTAAATTATTAATGATTTCATATTATAATATATGTATCTATATATTTTTAATGT
GTAATTCAAAATGGCGAAAAAGAAACAAACCATACTTTATAAGCAACTCTCTTTGAGCAC
TTACCATAAAAAATTATACTACTAAATTCAAATTAAATAAAAAAATATATATTATAAAAT
TGGCTTTAATTATTTATAAGCACTTGCTTTGTTTTACTCAAAAAGTATTGGCATAAGTAT
AAACATAAAACCAATTTTTCACAAAATATATTATATGTCCATACAACTATATCCGAACYT
TCAAAACACATCCAAATTTCCATTTACCACATGCAAGCAAAAGGATATTAAATAAAAAAA
AATTATTTATATTTTCATGATCACTTAAATAATATTTAAAAAAAAAAGAGACCATATATA
AAATGTATAATTTAAATATAGCTAACTTATTAAAAAAAATGAATATTGAGCATATATCCA
TAATTTAAAACACTTCTATTTTATGTTATTTGATATATAAGTCGATAAATTATTGCATGT
ATGCATATAGACAGATTAATATACACCAAAATAATAAAAATTATATATAGTTCATATATT
ATTCTGAATAATAAATTAGCTTTCCTCTTAATTCACTTGGTATGTATATATATATATATA
TATATATATATATATATATGGATAAATAAATATAAGGTCTATAACAGGAAAAAATGTGTA
TTAACTTTTTTTTATAATTATTTTATATTTTTTCTCCATTTATTTAAATATGTTAAAATT
ATTTCCCATTTTATAAACAAAAATTAATCCATAAAATAGCAAAATAACAAAATGACAAAA
TAACAAAAACAAAAAAACGCATTATATGAGTTCATTTTACACAATCCAAAGAAAAAGAAA
ATATGCTTAAAATATTCATAACACACTTTTAAGCTATAAAAATATATATATTTATTTGTG
TCTATATTACCAACTCAATTTAATAGATGTGTTATGTGATTAATTCATACACAAACATAC
AAAAATAAACACCATTTTTGAAAAGATTAATTTGAAATCATATATGTTATTATTATATAT
TTTTATATCAATGAAATTTAAAAAAATAAAAGGGAAATCAATGTATTAAAAATAATTATA
TGTTATTTTATTTCCACAATATTTATTTATTATTTATTGTTGACCTGCAGGCATGCAAGC
TTATCGCGATACCGTCGACCTCGAGGGGGGGCCCGGTACCCAGCTTTTGTTCCCTTTAGT
GAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTT
ATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTG
CCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGG
GAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGC
GTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGC
GGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATA
ACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCG
CGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCT
CAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAA
GCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTC
TCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGT
AGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCG
CCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGG
CAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCT
TGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGC
TGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCG
CTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTC
AAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTT
AAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAA
AATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAAT
GCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCT
GACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTG
CAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAG
CCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTA
ATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTG
CCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCG
GTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCT
CCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTA
TGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTG
GTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCC
CGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTG
GAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGA
TGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTG
GGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAAT
GTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTC
TCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCA
CATTTCCCCGAAAAGTGCCAC
Restriction sites to linearize plasmid ClaI
Selectable marker used to select the mutant parasitetgdhfr
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1TATGGGATTTTCACATATTTACAAC
Additional information primer 1L6437 (SpeI); PBANKA_021550 Forward
Sequence Primer 2TGGATCCTGGATAAACTTTATCTTTCAAATAATCTTC
Additional information primer 2L6438 (BamHI); PBANKA_021550 Reverse
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6
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Conceptual design of the database: Dr. C.J. Janse (Leiden Malaria Research Group, Leiden, The Netherlands).
Technical design and realization: StudioDrie StudioDrie; S. Mededovic and A. van Wigcheren