Mutant/mutation
The mutant expresses a C-terminal 6xHA-tagged version of PY17X_0831600.

Protein (function)
Cytoplasmic dynein comprises six subunits, including one HC, one IC, one LIC, and three LCs (Robl, LC8, and Tctex). We sought to identify all the subunits of subpellicular microtubules (SPMTs) cytoplasmic dynein in the ookinetes. In the P. yoelii genome, one putative dynein IC gene (PY17X_0505600), one putative dynein LIC gene (PY17X_0417700), and 12 putative dynein LC genes are encoded.
These 14 proteins (PY17X_1024300 Light chain (LC),  PY17X_1208500 Light chain (LC),  PY17X_0708400 Light chain (LC),  PY17X_1135100 Light chain (LC), PY17X_0302800 Light chain (LC),  PY17X_1241500 Light chain (LC),  PY17X_0935600 Light chain (LC),  PY17X_0215300 Light chain (LC), PY17X_0831600 Light chain (Tctex), PY17X_1431600 Light chain (LC8),  PY17X_0505400 Light chain (Robl),  PY17X_1040900 Light chain (LC),  PY17X_0505600 Intermediate chain (IC),  PY17X_0417700 Light Intermediate chain (LIC)), conserved in human and rodent Plasmodium species, have not been investigated for expression and localization.

The invasive “zoite” stages of Plasmodium, including the ookinete, sporozoite, and merozoite, are morphologically polarized and possess a unique cortical pellicle underneath the parasite plasma membrane. From outside to inside, the pellicle consists of a double membrane organelle inner membrane complex (IMC) and a cytoskeleton layer of apically radiating subpellicular microtubules (SPMTs), both of which associate with each other and span along the periphery of the zoite parasites. Besides SPMTs, the invasive zoites of apicomplexan parasites possess a highly specialized structure called the apical polar ring (APR) at the cell apical cortex. APR is recognized as an electron-lucent region beneath the apical IMC. it is believed that APR functions as a microtubule-organizing center (MTOC) for nucleating SPMTs at the Plasmodium zoites. In Plasmodium, the SPMT cytoskeleton functions as a scaffold supporting parasite morphogenesis, maintaining the polarized cell shapes and providing parasite rigidity during gliding and invasion. In the process of ookinete growth, the parasite undergoes massive expansion of the plasma and cortex membrane. In addition, the ookinete acquires a complete set of apical organelles and structures via de novo assembly. IMC is assembled at the apical site of the initial protrusion and extends along the expanding plasma membrane to the basal end. After biogenesis,APR nucleates the assembly of apical SPMTs underling the IMC. Meanwhile, the apical tubulin ring (ATR), another compacted structure of MTs, emerges at the apex of ookinetes.While the IMC, SPMT, APR, ATR, and microneme are essential for either development, gliding, or midgut invasion of ookinetes, the mechanisms for de novo assembly of these organelles and structures at the apical distal area are largely unknown. We hypothesize that besides playing a cytoskeleton role, the apically radiating SPMTs may function as the tracks for the apical transport of cargoes containing the contents required for the assembly of apical organelles and structures in the ookinetes. In this scenario, the cytoplasmic dynein could be the primary motor moving towards the minus end of SPMTs for cargo transport to the apical distal area of ookinetes. However, the SPMT-based dynein transport machinery has not been identified in Plasmodium. Cytoplasmic dynein is a large multi-subunit protein complex, and the core is a homodimer of two heavy chain subunits (DHCs) interacting with intermediate, light intermediate, and light chain subunits

Phenotype
No expression in asexual blood stages. Expression in ookinetes along the periphery of ookinetes. 

Cytoplasmic dynein comprises six subunits, including one HC, one IC, one LIC, and three LCs (Robl, LC8, and Tctex). We sought to identify all the subunits of SPMT cytoplasmic dynein in the ookinetes. In the P. yoelii genome, one putative dynein IC gene (PY17X_0505600), one putative dynein LIC gene (PY17X_0417700), and 12 putative dynein LC genes are encoded.
These 14 proteins (PY17X_1024300 Light chain (LC),  PY17X_1208500 Light chain (LC),  PY17X_0708400 Light chain (LC),  PY17X_1135100 Light chain (LC), PY17X_0302800 Light chain (LC),  PY17X_1241500 Light chain (LC),  PY17X_0935600 Light chain (LC),  PY17X_0215300 Light chain (LC), PY17X_0831600 Light chain (Tctex), PY17X_1431600 Light chain (LC8),  PY17X_0505400 Light chain (Robl),  PY17X_1040900 Light chain (LC),  PY17X_0505600 Intermediate chain (IC),  PY17X_0417700 Light Intermediate chain (LIC)), conserved in human and rodent Plasmodium species, have not been investigated for expression and localization. We tagged each of these candidate proteins with a 6HA at the C-terminus in the 17XNL using CRISPR-Cas9 and analyzed their expression and localization in ookinetes. IFA of the tagged parasite clones showed that five proteins (LIC, PY17X_0417700 RMgm-5623; IC, PY17X_0505600 RMgm-5624; three LCs, PY17X_0505400 RMgm-5625, PY17X_1431600 RMgm-5626, and PY17X_0831600 RMgm-5627) were localized at the periphery of ookinetes. In addition, these five proteins displayed similar localization dynamics during ookinete morphogenesis as the dynein heavy chain (DHC) protein (DHC3; see mutant RMgm-5615) and were not detected in the asexual blood stages. To confirm the co-localization of these candidate subunits with DHC3, we engineered parasite clones with each of these five proteins tagged with a 6HA from the 4Myc::dhc3 parasite and obtained five double-tagged parasite lines. Two-colored IFA showed these five proteins were co-localized with DHC3 in the periphery of ookinetes. To visualize the association of the protein with SPMTs in more detail, the ookinetes were stained with antibodies against HA and α/β-Tubulin and imaged by U-ExM. These subunits (LIC, IC, Robl, LC8, and Tctex) showed complete co-localization with SPMTs in the ookinetes. It is noted that these five subunits showed no signal at the ATR, consistent with the results of DHC3. These pieces of evidence indicate that the SPMT-based cytoplasmic dynein complex is composed of DHC3, LIC, IC, and three LCs (Robl, LC8, and Tctex).

Additional information
There are 7 genes encoding putative dynein heavy chain (DHC) proteins of Plasmodium parasites. In the rodent malaria parasite P. yoelii, they are PY17X_0418900 (dhc1), PY17X_0618400 (dhc2), PY17X_0215400 (dhc3), PY17X_0508400 (dhc4), PY17X_0927400 (dhc5), PY17X_0603800 (dhc6), and PY17X_1333900 (dhc7).
Each dynein contains at least one dynein heavy chain (DHC) and other subunits including the intermediate chain (IC), light intermediate chain (LIC), and light chain (LC).The seven dhc-genes were tagged with a sextuple HA epitope (6HA) to analyse the expression and localization of these proteins. See the mutants RMgm-5613 (PY17X_0418900-6HA; dhc1), RMgm-5614 (PY17X_0618400-6HA; dhc2), RMgm-5615 (PY17X_0215400-6HA; dhc3, RMgm-5616 (PY17X_0508400-6HA; dhc4), RMgm-5617 (PY17X_0927400-6HA; dhc5), RMgm-5618 (PY17X_0603800-6HA; dhc6), RMgm-5619 PY17X_1333900-6HA; dhc7). Immunofluorescence assay (IFA) showed that all 7 DHCs displayed no detectable expression in the asexual blood stages. Only DHC3 was expressed in female gametocytes, ookinetes, and sporozoites. The other 6 DHCs were specifically expressed in male gametocytes. DHC3 was evenly distributed along the periphery of ookinetes, suggesting the existence of SPMT-based dynein in the ookinetes.

From the paper:
The cortical cytoskeleton of subpellicular microtubules (SPMTs) supports the Plasmodium ookinete morphogenesis during mosquito transmission of malaria. SPMTs are hypothesized to function as the cytoskeletal tracks in motor-driven cargo transport for apical organelle and structure assembly in ookinetes. We identify the core subunit DHC3 and other subunits of dynein showing co-localizing with SPMTs in the ookinete. In-depth phenotypical and functional analyses demonstrate that SPMT-based dynein plays an essential role in ookinete morphogenesis, shape, and gliding motility. DHC3 disruption impairs ookinete development, shape, and gliding, leading to failure in mosquito infection of Plasmodium. The DHC3-deficient ookinetes display defective formation or localization of apical organelles and structures. Rab11A and Rab11B interact with DHC3 at SPMTs in a DHC3-dependent manner, likely functioning as the receptors for the cargoes driven by SPMT-dynein. Disturbing Rab11A or Rab11B phenocopies DHC3 deficiency in ookinete morphogenesis. This study confirms the existence of the SPMT-based cytoplasmic dynein motor in the ookinetes and reveals its importance in intracellular cargo transport in ookinete morphogenesis.

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