SummaryRMgm-5281
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Successful modification | The parasite was generated by the genetic modification |
The mutant contains the following genetic modification(s) | Gene tagging |
Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 36463257 |
MR4 number | |
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Parent parasite used to introduce the genetic modification | |
Rodent Malaria Parasite | P. yoelii |
Parent strain/line | P. y. yoelii 17XNL |
Name parent line/clone | Not applicable |
Other information parent line | |
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The mutant parasite was generated by | |
Name PI/Researcher | Quian P, Yuan J |
Name Group/Department | State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signal Network, School o |
Name Institute | Xiamen University |
City | Xiamen |
Country | China |
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Name of the mutant parasite | |
RMgm number | RMgm-5281 |
Principal name | apr2::6HA |
Alternative name | |
Standardized name | |
Is the mutant parasite cloned after genetic modification | Yes |
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Phenotype | |
Asexual blood stage | APR2 expression was detected in asexual blood stage, gametocyte, midgut oocyst, and salivary gland sporozoite. APR2 localizes at apical polar ring (APR) throughout zygote to ookinete development (see also below). Apical localization of APR2 was also observed in merozoites and sporozoites. |
Gametocyte/Gamete | APR2 expression was detected in asexual blood stage, gametocyte, midgut oocyst, and salivary gland sporozoite. APR2 localizes at apical polar ring (APR) throughout zygote to ookinete development (see also below). Apical localization of APR2 was also observed in merozoites and sporozoites. |
Fertilization and ookinete | APR2 expression was detected in asexual blood stage, gametocyte, midgut oocyst, and salivary gland sporozoite. APR2 localizes at apical polar ring (APR) throughout zygote to ookinete development (see also below). Apical localization of APR2 was also observed in merozoites and sporozoites. |
Oocyst | APR2 expression was detected in asexual blood stage, gametocyte, midgut oocyst, and salivary gland sporozoite. APR2 localizes at apical polar ring (APR) throughout zygote to ookinete development (see also below). Apical localization of APR2 was also observed in merozoites and sporozoites. |
Sporozoite | APR2 expression was detected in asexual blood stage, gametocyte, midgut oocyst, and salivary gland sporozoite. APR2 localizes at apical polar ring (APR) throughout zygote to ookinete development (see also below). Apical localization of APR2 was also observed in merozoites and sporozoites. |
Liver stage | Not tested |
Additional remarks phenotype | Mutant/mutation Phenotype Additional information Other mutants |
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Details of the target gene | |||||||||||||||||||||||||||
Gene Model of Rodent Parasite | PY17X_1339500 | ||||||||||||||||||||||||||
Gene Model P. falciparum ortholog | PF3D7_1471600 | ||||||||||||||||||||||||||
Gene product | apical polar ring protein APR2, putative | ||||||||||||||||||||||||||
Gene product: Alternative name | APR2 | ||||||||||||||||||||||||||
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Details of the genetic modification | |||||||||||||||||||||||||||
Name of the tag | 6xHA | ||||||||||||||||||||||||||
Details of tagging | C-terminal | ||||||||||||||||||||||||||
Additional remarks: tagging | We tagged the APR2 with a 6HA at either the N- or C-terminus in the 17XNL parasite using the CRISPR-Cas9 method, generating 6HA::apr2 and apr2::6HA clones | ||||||||||||||||||||||||||
Commercial source of tag-antibodies | |||||||||||||||||||||||||||
Type of plasmid/construct | CRISPR/Cas9 construct: integration through double strand break repair | ||||||||||||||||||||||||||
PlasmoGEM (Sanger) construct/vector used | No | ||||||||||||||||||||||||||
Modified PlasmoGEM construct/vector used | No | ||||||||||||||||||||||||||
Plasmid/construct map | |||||||||||||||||||||||||||
Plasmid/construct sequence | |||||||||||||||||||||||||||
Restriction sites to linearize plasmid | |||||||||||||||||||||||||||
Selectable marker used to select the mutant parasite | hdhfr/yfcu | ||||||||||||||||||||||||||
Promoter of the selectable marker | eef1a | ||||||||||||||||||||||||||
Selection (positive) procedure | pyrimethamine | ||||||||||||||||||||||||||
Selection (negative) procedure | No | ||||||||||||||||||||||||||
Additional remarks genetic modification | We tagged the APR2 with a 6HA at either the N- or C-terminus in the 17XNL parasite using the CRISPR-Cas9 method, generating 6HA::apr2 and apr2::6HA clones | ||||||||||||||||||||||||||
Additional remarks selection procedure | |||||||||||||||||||||||||||
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