RMgmDB - Rodent Malaria genetically modified Parasites


Malaria parasiteP. berghei
TaggedGene model (rodent): PBANKA_1400700; Gene model (P.falciparum): Not available; Gene product: Plasmodium exported protein, unknown function
Name tag: mCherry
Phenotype Asexual bloodstage; Liver stage;
Last modified: 21 September 2022, 12:18
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene tagging
Reference (PubMed-PMID number) Reference 1 (PMID number) : 36126089
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone unknown
Other information parent line
The mutant parasite was generated by
Name PI/ResearcherGabelich JA, Ingmundson A
Name Group/DepartmentMolecular Parasitology
Name InstituteHumboldt University
Name of the mutant parasite
RMgm numberRMgm-5229
Principal namePBANKA_1400700-mCherry
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationYes
Asexual blood stagePBANKA_1400700 localizes to punctate structures in the cytoplasm of infected red blood cells
Gametocyte/GameteNot tested
Fertilization and ookineteNot tested
OocystNot tested
SporozoiteNot tested
Liver stagePBANKA_1400700-mCherry was detected throughout the liver stage, but it was not secreted from the parasite despite the presence of a signal peptide.
Additional remarks phenotype

The mutant expresses a C-terminal mCherry-tagged version of PBANKA_1400700.

Protein (function)
Plasmodium exported protein, unknown function.

PBANKA_1400700 localizes to punctate structures in the cytoplasm of infected red blood cells.
PBANKA_1400700-mCherry was detected throughout the liver stage, but it was not secreted from the parasite despite the presence of a signal peptide.

Additional information
PBANKA_0524300 (IPIS2), PBANKA_0623100 (IPIS3) and PBANKA_1400700, were found to be localized to IBIS1-positive membranes in the cytoplasm of infected red blood cells. The first two proteins both in red blood cells and (in the PVM of) liver stages. These proteins were termed intra-erythrocytic Plasmodium-induced structure proteins 2 and 3 (IPIS2 and IPIS3, respectively). PBANKA_1400700 was only exported into red blood cells but remained in the cytoplasm of liver stages.

Analyses of mCherry-tagged proteins (see RMgm-5227RMgm-5228RMgm-5229) showed the following:

- IPIS2 and IPIS3 localizes to punctate structures in the cytoplasm of infected red blood cells
- A previous study reported exported GFP-tagged PBANKA_1400700 evenly distributed throughout the erythrocyte cytoplasm. In contrast, we detected the exported fusion protein in a punctate pattern. In both this study and the previous study, much of the fluorescently tagged PBANKA_1400700 remained associated with the parasites.
- All three proteins were also detected in the liver infection stage. PBANKA_0524300-mCherry and PBANKA_0623100-mCherry were first detected 24 hours post-infection in the developing liver-stage forms and surround the parasite in a pattern indicative of the liver-stage parasitophorous vacuole and associated tubovesicular network. PBANKA_1400700-mCherry was detected throughout the liver stage, but it was not secreted from the parasite despite the presence of a signal peptide.
- To determine if the localization of these three proteins overlaps with IBIS1, the parasite lines in which these proteins are tagged with mCherry were crossed with a  line in which IBIS1 is tagged with GFP via co-infections of mosquitoes. Signals from PBANKA_0524300-mCherry and PBANKA_0623100-mCherry overlapped considerably with the IBIS1-GFP signal surrounding the liver-stage parasites and in infected red blood cells, colocalize with IBIS1-GFP. 
Although the majority of PBANKA_1400700-mCherry remains parasite-associated, the exported mCherry signal colocalizes with IBIS1-GFP.

In order to determine if the putative orthologs from P. vivax also targeted the membrane structures in the cytoplasm of Plasmodium-infected red blood cells, we generated P. berghei lines that expressed HA-tagged PvTRAg8, the syntenic P. vivax ortholog of IPIS2 (PVP01_0532600) or HA-tagged PvTRAg2, which shares similarity to IPIS3 (PVP01_0202200). These genes were integrated into the P. berghei genome such that they are expressed under the control of the IPIS2 or IPIS3 promoters, respectively. Both PvTRAg8-HA and PvTRAg2-HA are exported efficiently by P. berghei and localize to discrete structures in the cytoplasm of infected erythrocytes.

The localization of IPIS2 and IPIS3 to the intraerythrocytic membrane structures and their requirement for efficient blood-stage growth prompted us to investigate whether the membrane structures are still formed in the cytoplasm of erythrocytes infected with the ipis2- and ipis3- knockout lines. In order to visualize these structures in the knockout lines, we tagged IPIS2 with the fluorescent protein mCherry in the IPIS3-deficient line, and likewise, tagged IPIS3 with mCherry in the line lacking IPIS2. The IPIS proteins still appear in punctate structures distributed across the erythrocyte cytoplasm in the absence of either IPIS2 or IPIS3

Other mutants

  Tagged: Mutant parasite with a tagged gene
Details of the target gene
Gene Model of Rodent Parasite PBANKA_1400700
Gene Model P. falciparum ortholog Not available
Gene productPlasmodium exported protein, unknown function
Gene product: Alternative name
Details of the genetic modification
Name of the tagmCherry
Details of taggingC-terminal
Additional remarks: tagging
Commercial source of tag-antibodies
Type of plasmid/construct(Linear) plasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Selectable marker used to select the mutant parasitetgdhfr
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modificationFor the construction of the plasmids used for endogenous C-terminal mCherry tagging, regions containing the IPIS2, IPIS3 or PBANKA_1400700 genes were amplified from P. berghei genomic DNA using the respective primers and integrated into the SacII and XbaI sites of the B3D+mCherry plasmid. The sequences of PvTRAg8 and PvTRAg2 were codon-optimized synthesized (GenScript) and cloned into the NotI and SpeI sites of b3D.DT^H.^D. The 5’ and 3’ untranslated regions of the IPIS2 and IPIS3 genes were cloned into the GOMO-GFP luciferase vector using the respective primers containing SacII and NotI sites and the XhoI and KpnI sites, respectively, in order to delete IPIS2 and IPIS3 via double homologous recombination.
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6