Mutant/mutation
The mutant expresses a C-terminal mCherry-tagged version of UBC13

Protein (function)
The process of ubiquitylation typically requires three enzymes: E1 (ubiquitin-activating enzyme, Uba), E2 (ubiquitin-conjugating enzyme, Ubc), and E3 (ubiquitin-protein ligase). Amongst Ubcs, Ubc13 specifically catalyzes the formation of K63-linked ubiquitin chains that are critical to signaling in inflammatory and DNA damage response pathways.

Phenotype
Cytoplasmic expression of Ubc13-mCherry in blood stages, oocysts, sporozoite stages and liver stages

Additional information
See also mutant RMgm-5206 lacking expression of PK9. Previous studies have shown that functionally Pk9 can be designated as a serine-threonine kinase (STK) owing to its ability to phosphorylate E2 conjugation enzyme at serine 106 residue of Ubc13, although it does not cluster with any established eukaryotic protein kinase groups and hence is referred to as orphan kinase. In this study evidence is presented that that phosphorylation of the regulatory serine 106 of Ubc13 is essential for the completion of the parasite’s erythrocytic cycle and observed phosphorylated form of Ubc13 in Pk9 knockout parasites.

See also mutant RMgm-5208 with a mutated UNC13 (the phosphorylating serine 106 replaced with with non-phosphorylatable alanine).

Other mutants