RMgmDB - Rodent Malaria genetically modified Parasites


Malaria parasiteP. berghei
TaggedGene model (rodent): PBANKA_1347400; Gene model (P.falciparum): PF3D7_1332600; Gene product: DNA-(apurinic or apyrimidinic site) lyase 1 (APN1)
Name tag: 3xHA-mCherry
Phenotype Asexual bloodstage; Oocyst;
Last modified: 13 May 2021, 16:58
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene tagging
Reference (PubMed-PMID number) Reference 1 (PMID number) : 33711786
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone Not applicable
Other information parent line
The mutant parasite was generated by
Name PI/ResearcherSrivastava PN, Mishra S
Name Group/DepartmentDivision of Molecular Parasitology and Immunology
Name InstituteCSIR-Central Drug Research Institute
Name of the mutant parasite
RMgm numberRMgm-4985
Principal nameApn1(mch)
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationYes
Asexual blood stageExpression of mCherry-tagged APN1 in schizonts with mitochondrial location
Gametocyte/GameteNot different from wild type
Fertilization and ookineteNot different from wild type
OocystExpression of mCherry-tagged APN1 in mature oocysts containing sporozoites
SporozoiteNot different from wild type
Liver stageNot different from wild type
Additional remarks phenotype

The mutant expresses a C-terminal 3xHA-mCherry tagged version of APN1

Protein (function)
AP endonucleases are components of DNA repair via the base excision repair (BER) pathway. Plasmodium berghei DNA-(apurinic or apyrimidinic site) lyase 1, putative (Apn1, PBANKA_1347400) is one of the two AP endonucleases found in its genome. The main function of Apn1 is to generate a nick at the 5′-phosphodiester bond in the AP site and generate free 5′-phosphate and 3′-hydroxyl groups.

No phenotype detected in a mutant lacking expression of APN1 (RMgm-4984)

Evidence for localization of mCherry-tagged APN1 in mitochondria of schizonts and oocyst sporozoites

Additional information

Other mutants

  Tagged: Mutant parasite with a tagged gene
Details of the target gene
Gene Model of Rodent Parasite PBANKA_1347400
Gene Model P. falciparum ortholog PF3D7_1332600
Gene productDNA-(apurinic or apyrimidinic site) lyase 1
Gene product: Alternative nameAPN1
Details of the genetic modification
Name of the tag3xHA-mCherry
Details of taggingC-terminal
Additional remarks: tagging
Commercial source of tag-antibodies
Type of plasmid/construct(Linear) plasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Selectable marker used to select the mutant parasitehdhfr
Promoter of the selectable markereef1a
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6