RMgmDB - Rodent Malaria genetically modified Parasites

Summary

RMgm-4984

Malaria parasite	P. berghei
Genotype
Disrupted	Gene model (rodent): PBANKA_1347400; Gene model (P.falciparum): PF3D7_1332600; Gene product: DNA-(apurinic or apyrimidinic site) lyase 1 (APN1)
Phenotype	No phenotype has been described

Last modified: 13 May 2021, 16:45

*RMgm-4984

Successful modification	The parasite was generated by the genetic modification
The mutant contains the following genetic modification(s)	Gene disruption
Reference (PubMed-PMID number)	Reference 1 (PMID number) : 33711786
MR4 number
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Parent parasite used to introduce the genetic modification
Rodent Malaria Parasite	P. berghei
Parent strain/line	P. berghei ANKA
Name parent line/clone	Not applicable
Other information parent line
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The mutant parasite was generated by
Name PI/Researcher	Srivastava PN, Mishra S
Name Group/Department	Division of Molecular Parasitology and Immunology
Name Institute	CSIR-Central Drug Research Institute
City	Lucknow
Country	India
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Name of the mutant parasite
RMgm number	RMgm-4984
Principal name	Apn1(-)
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modification	Yes
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Phenotype
Asexual blood stage	Not different from wild type
Gametocyte/Gamete	Not different from wild type
Fertilization and ookinete	Not different from wild type
Oocyst	Not different from wild type
Sporozoite	Not different from wild type
Liver stage	Not different from wild type
Additional remarks phenotype	Mutant/mutation the mutant lacks expression of APN1 (and expresses GFP) Protein (function) AP endonucleases are components of DNA repair via the base excision repair (BER) pathway. Plasmodium berghei DNA-(apurinic or apyrimidinic site) lyase 1, putative (Apn1, PBANKA_1347400) is one of the two AP endonucleases found in its genome. The main function of Apn1 is to generate a nick at the 5′-phosphodiester bond in the AP site and generate free 5′-phosphate and 3′-hydroxyl groups. Phenotype No phenotype detected Additional information Evidence for localization in mitochondria of schizonts and oocyst sporozoites (see RMgm-4985). Evidence is presented that Apn1− parasites maintain the integrity of mitochondrial DNA Other mutants

Disrupted: Mutant parasite with a disrupted gene

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Details of the target gene

Gene Model of Rodent Parasite

PBANKA_1347400

Gene Model P. falciparum ortholog

PF3D7_1332600

Gene product

DNA-(apurinic or apyrimidinic site) lyase 1

Gene product: Alternative name

APN1

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Details of the genetic modification

Inducable system used

Additional remarks inducable system

Type of plasmid/construct used

(Linear) plasmid double cross-over

PlasmoGEM (Sanger) construct/vector used

Modified PlasmoGEM construct/vector used

Plasmid/construct map

Plasmid/construct sequence

Restriction sites to linearize plasmid

Partial or complete disruption of the gene

Complete

Additional remarks partial/complete disruption

Selectable marker used to select the mutant parasite

hdhfr/yfcu

Promoter of the selectable marker

eef1a

Selection (positive) procedure

pyrimethamine

Selection (negative) procedure

Additional remarks genetic modification

Additional remarks selection procedure

Primer information: Primers used for amplification of the target sequences Click to view information

Primer information: Primers used for amplification of the target sequences Click to hide information

Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6

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