RMgmDB - Rodent Malaria genetically modified Parasites


Malaria parasiteP. berghei
DisruptedGene model (rodent): PBANKA_1347400; Gene model (P.falciparum): PF3D7_1332600; Gene product: DNA-(apurinic or apyrimidinic site) lyase 1 (APN1)
PhenotypeNo phenotype has been described
Last modified: 13 May 2021, 16:45
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene disruption
Reference (PubMed-PMID number) Reference 1 (PMID number) : 33711786
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone Not applicable
Other information parent line
The mutant parasite was generated by
Name PI/ResearcherSrivastava PN, Mishra S
Name Group/DepartmentDivision of Molecular Parasitology and Immunology
Name InstituteCSIR-Central Drug Research Institute
Name of the mutant parasite
RMgm numberRMgm-4984
Principal nameApn1(-)
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationYes
Asexual blood stageNot different from wild type
Gametocyte/GameteNot different from wild type
Fertilization and ookineteNot different from wild type
OocystNot different from wild type
SporozoiteNot different from wild type
Liver stageNot different from wild type
Additional remarks phenotype

the mutant lacks expression of APN1 (and expresses GFP)

Protein (function)
AP endonucleases are components of DNA repair via the base excision repair (BER) pathway. Plasmodium berghei DNA-(apurinic or apyrimidinic site) lyase 1, putative (Apn1, PBANKA_1347400) is one of the two AP endonucleases found in its genome. The main function of Apn1 is to generate a nick at the 5′-phosphodiester bond in the AP site and generate free 5′-phosphate and 3′-hydroxyl groups.

No phenotype detected

Additional information
Evidence for localization in mitochondria of schizonts and oocyst sporozoites (see RMgm-4985).
Evidence is presented that Apn1− parasites maintain the integrity of mitochondrial DNA

Other mutants

  Disrupted: Mutant parasite with a disrupted gene
Details of the target gene
Gene Model of Rodent Parasite PBANKA_1347400
Gene Model P. falciparum ortholog PF3D7_1332600
Gene productDNA-(apurinic or apyrimidinic site) lyase 1
Gene product: Alternative nameAPN1
Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct used(Linear) plasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Partial or complete disruption of the geneComplete
Additional remarks partial/complete disruption
Selectable marker used to select the mutant parasitehdhfr/yfcu
Promoter of the selectable markereef1a
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6