Mutant/mutation
The mutant expresses of C-terminal, GFP-tagged version of rpa1 and a C-terminal, mCherry-tagged version of CCp2, LAP4.
The gene has been tagged using CRISPR/cas9 genome editing (see mutant RMgm-1096 for details).

Protein (function)

Phenotype
Protein expression of upregulated male genes in the AP2-O3 null female gametocytes
From the paper: ' We selected 4: dpod2 (PY17X_0408600, DNA polymerase delta small subunit), dpod1 (PY17X_0502300, DNA polymerase delta catalytic subunit), rpa1 (PY17X_0419400, replication protein A1 small fragment), and mcm7 (PY17X_0805800, DNA replication licensing factor). Each of these 4 genes was endogenously tagged with a gfp-coding sequence at the C-terminus in the female-identity reporter strain ccp2::mCherry, yielding 4 double-tagged strains, including ccp2::mCherry;dpod2::gfp (DTS1), ccp2::mCherry;dpod1::gfp (DTS2), ccp2::mCerry;rpa1::gfp (DTS3), and ccp2::mCherry;mcm7::gfp (DTS4).
All the 4 GFP-fusion proteins were expressed and nuclear localized in the male gametocytes , but not were detectable (Dpod2::GFP and Dpod1::GFP) or present at extremely low abundance (Rpa1::GFP and Mcm7::GFP) in the female gametocytes. Next, we removed ap2-o3 gene in each individual DTS strain. As expected, AP2-O3 disruption (see RMgm-4979) did not significantly affect the level of any of these 4 proteins in the male gametocytes as revealed by both fluorescence microscopy and flow cytometry In clear contrast, protein levels of Dpod2::GFP and Dpod1::GFP in the female gametocytes were dramatically increased, while Rpa1::GFP and Mcm7::GFP levels in the female gametocytes showed a modest increase compared to the corresponding parental strains.'

Additional information

Other mutants