Mutant/mutation
The mutant expresses a C-terminal mCherry-tagged version of CCp2. The mutant does not contain a drug-selectable marker

For tagging CCp2 the CRISPR/Cas9 method was used as described for mutants RMgm-1095 and RMgm-4071.

Protein (function)
The lap4 (ccp2) gene is a member of a small conserved gene family (6 genes), encoding proteins with multiple adhesive domains, for example a Lgl1 (LCCL)-lectin adhesive domain. In P. falciparum the LCCL domain-containing proteins are termed PfCCp's and in P. berghei PbLAP's

CCp2 is a female specific protein, transcribed/expressed in female gametocytes, ookinetes and young gametocytes

Phenotype
CCp2::mCherry expression in female gametocytes and ookinetes

Additional information
This mutant was used to cross with a mutant expressing GFP in male gametocytes (RMgm-4476), resulting in selection of a mutant with GFP-expressing males (green) and mCherry expressing females (red) (RMgm-4477). All mutants are drug-selectable marker free.

In P. berghei transcripts of ccp2 are under translational control in female gametocytes. The protein is only expressed after activation of gametocytes (in gametes/zygotes/ookinetes). In the mutants described here the mCherry-tagged version of P. yoelii ccp2 is expressed as a protein in female gametocytes.

Other mutants