SummaryRMgm-4117
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Successful modification | The parasite was generated by the genetic modification |
The mutant contains the following genetic modification(s) | Gene disruption |
Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 28115054 |
MR4 number | |
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Parent parasite used to introduce the genetic modification | |
Rodent Malaria Parasite | P. berghei |
Parent strain/line | P. berghei ANKA |
Name parent line/clone | Not applicable |
Other information parent line | |
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The mutant parasite was generated by | |
Name PI/Researcher | Klug D, Frischknecht F |
Name Group/Department | Integrative Parasitology, Center for Infectious Diseases |
Name Institute | Heidelberg University Medical School |
City | Heidelberg |
Country | Germany |
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Name of the mutant parasite | |
RMgm number | RMgm-4117 |
Principal name | trp1(-) |
Alternative name | |
Standardized name | |
Is the mutant parasite cloned after genetic modification | Yes |
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Phenotype | |
Asexual blood stage | Not different from wild type |
Gametocyte/Gamete | Not different from wild type |
Fertilization and ookinete | Not different from wild type |
Oocyst | Normal sporozoite formation in oocysts. Sporozoites were immotile inside oocysts. No egress of sporozoites. No salivary gland sporozoites. Sporozoites collected from oocysts are motile and infectious to mice after intravenous injection |
Sporozoite | Normal sporozoite formation in oocysts. Sporozoites were immotile inside oocysts. No egress of sporozoites. No salivary gland sporozoites. Sporozoites collected from oocysts are motile and infectious to mice after intravenous injection |
Liver stage | Normal sporozoite formation in oocysts. Sporozoites were immotile inside oocysts. No egress of sporozoites. No salivary gland sporozoites. Sporozoites collected from oocysts are motile and infectious to mice after intravenous injection |
Additional remarks phenotype | Mutant/mutation
Combined these phenotype analyses showed the following:
Additional information |
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Details of the target gene | |||||||||||||||||||||||||
Gene Model of Rodent Parasite | PBANKA_0707900 | ||||||||||||||||||||||||
Gene Model P. falciparum ortholog | PF3D7_0822700 | ||||||||||||||||||||||||
Gene product | thrombospondin-related protein 1, putative | ||||||||||||||||||||||||
Gene product: Alternative name | TRP1 | ||||||||||||||||||||||||
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Details of the genetic modification | |||||||||||||||||||||||||
Inducable system used | No | ||||||||||||||||||||||||
Additional remarks inducable system | |||||||||||||||||||||||||
Type of plasmid/construct used | (Linear) plasmid double cross-over | ||||||||||||||||||||||||
PlasmoGEM (Sanger) construct/vector used | No | ||||||||||||||||||||||||
Modified PlasmoGEM construct/vector used | No | ||||||||||||||||||||||||
Plasmid/construct map | |||||||||||||||||||||||||
Plasmid/construct sequence | |||||||||||||||||||||||||
Restriction sites to linearize plasmid | |||||||||||||||||||||||||
Partial or complete disruption of the gene | Complete | ||||||||||||||||||||||||
Additional remarks partial/complete disruption | |||||||||||||||||||||||||
Selectable marker used to select the mutant parasite | hdhfr/yfcu | ||||||||||||||||||||||||
Promoter of the selectable marker | eef1a | ||||||||||||||||||||||||
Selection (positive) procedure | pyrimethamine | ||||||||||||||||||||||||
Selection (negative) procedure | No | ||||||||||||||||||||||||
Additional remarks genetic modification | |||||||||||||||||||||||||
Additional remarks selection procedure | |||||||||||||||||||||||||
Primer information: Primers used for amplification of the target sequences
Primer information: Primers used for amplification of the target sequences
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