| Successful modification | The parasite was generated by the genetic modification |
| The mutant contains the following genetic modification(s) |
Gene tagging
|
| Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 18551171
|
| MR4 number |
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| Parent parasite used to introduce the genetic modification |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | P. berghei ANKA |
| Name parent line/clone |
P. berghei ANKA 507cl1 (RMgm-7)
|
| Other information parent line | P.berghei ANKA 507cl1 (RMgm-7) is a reference ANKA mutant line which expresses GFP under control of a constitutive promoter (PubMed: PMID: 16242190). |
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| The mutant parasite was generated by |
| Name PI/Researcher | O. Silvie; K. Matuschewski |
| Name Group/Department | Department of Parasitology |
| Name Institute | Heidelberg University School of Medicine |
| City | Heidelberg |
| Country | Germany |
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| Name of the mutant parasite |
| RMgm number | RMgm-186 |
| Principal name | slarp/mcherry |
| Alternative name | |
| Standardized name | |
| Is the mutant parasite cloned after genetic modification | Yes |
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| Phenotype |
| Asexual blood stage | Not different from wild type |
| Gametocyte/Gamete | Not different from wild type |
| Fertilization and ookinete | Not different from wild type |
| Oocyst | Not different from wild type |
| Sporozoite | Sporozoites show (a weak) mCherry-fluorescence associated with the nucleus of sporozoites |
| Liver stage | Not tested |
| Additional remarks phenotype | Mutant/mutation
The mutant expresses an mCherry-tagged form of SLARP (sporozoite and liver stage asparagine-rich protein; SAP1, sporozoite asparagine-rich protein; S22).
Protein (function)
SLARP was first identified as a sporozoite-expressed gene in a suppression subtractive hybridization (SSH) screen of P. yoelii salivary gland sporozoites versus blood-stage merozoites (designated S22, sporozoite-specific gene 22).
See RMgm-172 and RMgm-185 for mutants lacking expression of SLARP/SAP1.
Phenotype
Sporozoites show (a weak) mCherry-fluorescence associated with the nucleus of sporozoites (see also 'Additional information'). No fluorescence was detected in blood stages and oocysts.
Additional information
The nucleus associated fluorescence indicates a nuclear location of SLARP/SAP1.
In an independent study IFA analysis using antibodies against SAP1 a specific sporozoite-internal staining was observed that excluded the nucleus and was distinct from circumsporozoite (CS) protein staining, suggesting a cytoplasmic location (see RMgm-172).
Other mutants
RMgm-172, RMgm-185: Mutants lacking expression of SAP1/SLARP |
*RMgm-186
