| Successful modification | The parasite was generated by the genetic modification |
| The mutant contains the following genetic modification(s) |
Gene disruption,
Gene disruption,
Gene disruption
|
| Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 26222913
|
| MR4 number |
|
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| Parent parasite used to introduce the genetic modification |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | P. berghei ANKA |
| Name parent line/clone |
P. berghei ANKA 2.34
|
| Other information parent line | P. berghei ANKA 2.34 is a cloned, gametocyte producer line of the ANKA strain (PubMed: PMID: 15137943). |
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| The mutant parasite was generated by |
| Name PI/Researcher | Andreadaki M; Siden-Kiamos I |
| Name Group/Department | Department of Biology |
| Name Institute | University of Crete |
| City | Heraklion |
| Country | Greece |
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| Name of the mutant parasite |
| RMgm number | RMgm-1319 |
| Principal name | Δtriplemsp7 |
| Alternative name | |
| Standardized name | |
| Is the mutant parasite cloned after genetic modification | Yes |
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| Phenotype |
| Asexual blood stage | The selection of mutants lacking all three MSP7-related genes indicate that these genes do not have an essential function for blood stage development. In this study the blood stage growth/multiplication and red blood cell invasion have not been analysed in detail. |
| Gametocyte/Gamete | Not different from wild type |
| Fertilization and ookinete | Not tested |
| Oocyst | Not different from wild type |
| Sporozoite | Not different from wild type |
| Liver stage | Not different from wild type |
| Additional remarks phenotype | Mutant/mutation
The mutant lacks expression of all 3 MSP7-related proteins: PBANKA_1349000 (MSP7-like protein, MSRP2), PBANKA_1349100 (merozoite surface protein 7; MSP7), PBANKA_1349200 ((MSP7-like protein; MSRP1)
Protein (function)
In all Plasmodium genomes examined, msp7 is a member of a small family of related genes located at the same locus. For example, the genome of P. falciparum contains MSP7and 7 MSP7-related genes (MSRP genes). Rodent parasites contain MSP7-related genes. The exact orthology between human and rodent members is not clear.
In P. falciparum MSP7 (PF13_0197) is a protein of the surface coat of merozoites. The surface coat of the merozoite is composed of a number of proteins, the most abundant of which is a complex of MSP1, MSP6, and MSP7. Glycosylphosphatidyl inositol (GPI) membrane-anchored MSP1 is processed during maturation of merozoites and erythrocyte invasion. Following invasion, only a C-terminal 19-kDa fragment (MSP119) is retained on the parasite membrane within the newly invaded erythrocyte. The rest of the MSP1 protein is shed as a protein complex comprising four polypeptides of MSP1 and associated with 36- and 22-kDa polypeptides derived from MSP6 and MSP7, respectively
Phenotype
The selection of mutants lacking all three MSP7-related genes indicate that these genes do not have an essential function for blood stage development. In this study the blood stage growth/multiplication and red blood cell invasion have not been analysed in detail. Previous analyses of mutants lacking expression of MSP7 (PBANKA_1349100) indicate a slight reduction in growth of asexual blood stages (RMgm-671, RMgm-673, RMgm-224). The mutant parasites were viable throughout the complete life cycle, including mosquito and liver stage development.
Additional information
Other mutants
Previous analyses of mutants lacking expression of MSP7 (PBANKA_1349100) indicate a slight reduction in growth of asexual blood stages (RMgm-671, RMgm-673, RMgm-224). |
*RMgm-1319
