RMgmDB - Rodent Malaria genetically modified Parasites

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Summary

RMgm-750
Malaria parasiteP. berghei
Genotype
Transgene
 Transgene not Plasmodium: GFP-Luciferase
Promoter: Gene model: PBANKA_1326400; Gene model (P.falciparum): PF3D7_1462800; Gene product: glyceraldehyde-3-phosphate dehydrogenase
3'UTR: Gene model: PBANKA_0719300; Gene product: bifunctional dihydrofolate reductase-thymidylate synthase, putative (dhfr/ts)
Replacement locus: Gene model: PBANKA_0306000; Gene product: 6-cysteine protein (230p)
Phenotype Sporozoite;
Last modified: 12 July 2012, 09:18
  *RMgm-750
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Introduction of a transgene
Reference (PubMed-PMID number) Reference 1 (PMID number) : 22574152
MR4 number
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Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone P. berghei ANKA cl15cy1
Other information parent lineA reference wild type clone from the ANKA strain of P. berghei (PubMed: PMID: 17406255).
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The mutant parasite was generated by
Name PI/ResearcherC. Ramakrishnan; E.A. Levashina
Name Group/DepartmentInstitut de Biologie Moléculaire et Cellulaire
Name InstituteUniversité de Strasbourg
CityStrasbourg
CountryFrance
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Name of the mutant parasite
RMgm numberRMgm-750
Principal nameglyc::GFP-LUC clone 2.5
Alternative name818
Standardized name
Is the mutant parasite cloned after genetic modificationYes
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Phenotype
Asexual blood stageNot different from wild type
Gametocyte/GameteNot different from wild type
Fertilization and ookineteNot different from wild type
OocystNot different from wild type
SporozoiteNo GFP was detected in non-sporulating or sporulating oocysts at 11 and 21 days post infection.
Salivary gland sporozoites expressed GFP (and luciferase).
Liver stageNot tested
Additional remarks phenotype

Mutant/mutation
The mutant expresses the fusion protein GFP-Luciferase under the control of the promoter of glyceraldehyde-3-phospho-dehydrogenase (glyc). The gfp-luciferase transgene has been integrated into the silent 230p locus

Phenotype
In blood stage ring-forms, trophozoites, gametocytes and ookinetes no GFP signal above background was observed for glyc::GFP-LUC. A faint but distinct GFP signal was observed in developing schizonts.
No GFP was detected in non-sporulating or sporulating oocysts at 11 and 21 days post infection. Salivary gland sporozoites expressed GFP (and luciferase).

Additional information
The luciferase activity of this reporter line and reporter line RMgm-749 has been exploited to establish a simple and fast biochemical assay to evaluate salivary gland sporozoite loads in whole mosquitoes

Other mutants
RMgm-749: A mutant expresses the fusion protein GFP-Luciferase under the control of the promoter of uis3 promoter. The gfp-luciferase transgene has been integrated into the silent 230p locus. Also in this mutant no GFP was detected in non-sporulating or sporulating oocysts at 11 and 21 days post infection. Salivary gland sporozoites expressed GFP (and luciferase).

  Transgene: Mutant parasite expressing a transgene
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Type and details of transgene
Is the transgene Plasmodium derived Transgene: not Plasmodium
Transgene nameGFP-Luciferase
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Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/constructPlasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
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Plasmid/construct sequence
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TCGAGAAGAGAAGGAAGACTGACGACGCAGCCACTGCGGAACCGAGCAACGCAATGAGCT
CCTTGACGTCCACGAGGGAGACAACTCCCGTGCACGGGTTGCAGGCTCCTTCTTCGGCCG
CAGCCATTGCCCCGGTGTTGGCGTGGATGGACGAAGAAGACCGGAAAAAACGCGAGCAAA
AGGAACTGATTCGGGCCGTTCCGCATGTTCACTTTAGAGGCCATGAAGAGTTCCAGTACC
TTGATCTCATTGCCGACATTATTAACAATGGAAGGACAATGGATGACCGAACGGGCGTTG
GTGTCATCTCCAAATTCGGCTGCACTATGCGCTACTCGCTGGATCAGGCCTTTCCACTTC
TCACCACAAAGCGTGTGTTCTGGAAAGGGGTCCTCGAAGAGTTGCTGTGGTTCATTCGCG
GCGACACGAACGCAAACCATCTTTCTGAGAAGGGCGTGAAGATCTGGGACAAGAATGTGA
CACGCGAGTTCCTCGATTCGCGCAATCTCCCCCACCGAGAGGTCGGAGACATCGGCCCGG
GCTACGGCTTCCAGTGGAGACACTTCGGCGCGGCATACAAAGACATGCACACAGACTACA
CAGGGCAGGGCGTCGACCAGCTGAAGAATGTGATCCAGATGCTGAGAACGAATCCAACAG
ATCGTCGCATGCTCATGACTGCCTGGAATCCTGCAGCGCTGGACGAAATGGCGCTGCCGC
CTTGTCACTTGTTGTGCCAGTTCTACGTGAACGACCAGAAGGAGCTGTCGTGCATCATGT
ATCAGCGGTCGTGCGATGTCGGCCTCGGCGTCCCCTTCAACATCGCTTCCTATTCGCTTT
TGACGCTCATGGTTGCACACGTCTGCAACCTAAAACCTAAGGAGTTCATTCACTTCATGG
GGAACACGCATGTCTACACGAACCATGTCGAGGCTTTAAAAGAGCAGCTGCGGAGAGAAC
CGAGACCGTTCCCCATTGTGAACATCCTCAACAAGGAACGCATCAAGGAAATCGACGATT
TCACCGCCGAGGATTTTGAGGTCGTGGGCTACGTCCCGCACGGACGAATCCAGATGGAGA
TGGCTGTCTAGCGGAAATACAGAAGCTAGCTTTGATCCCGTTTTTCTTACTTATATATTT
ATACCAATTGATTGTATTTATAACTGTAAAAATGTGTATGTTGTGTGCATATTTTTTTTT
GTGCATGCACATGCATGTAAATAGCTAAAATTATGAACATTTTATTTTTTGTTCAGAAAA
AAAAAACTTTACACACATAAAATGGCTAGTATGAATAGCCATATTTTATATAAATTAAAT
CCTATGAATTTATGACCATATTAAAAATTTAGATATTTATGGAACATAATATGTTTGAAA
CAATAAGACAAAATTATTATTATTATTATTATTTTTACTGTTATAATTATGTTGTCTCTT
CAATGATTCATAAATAGTTGGACTTGATTTTTAAAATGTTTATAATATGATTAGCATAGT
TAAATAAAAAAAGTTGAAAAATTAAAAAAAAACATATAAACACAAATGATGTTTTTTCCT
TCAATTTCGATATCGCGGCCGCGTATAAACTGAGTTAAGGGAAGTGGAAAATAATAAGCG
AAATACATTGGTGATTGCACAATACAAATATTTAATGTATATAAATATATACTTATTTAG
TTTTCATTTCTTCAAAAACGATATATATAATTTGGATAATAAAATCGTATTCTAATTATA
TATAAAAACTATCATATTTTTATATTCAAGAAGTTATCTTAGTATATCAGGTTAATCAAA
AATAAAATACTTATTTTCCATGCCCCAATTTATATGTACTCAAAGGGTAAAAAGTGGGAA
TATTATCTTACATTAGCAAAGTTTTTTATTATAAAATTCGAATTACTTAAGAAATGTATT
TTATTCTATTACATAAATTAAAAGTAAAAAAACATTATAATCAATAAATATTAATATATG
GAAAATACATCAAGCACAAATATATAACTGAAAATGGCAGTATTCAATTATTTTAATAAC
TCCAAAACATATTTTGTTCCTATTATATTTTATATGTAAAATAGTGAAATAGTTAAAATA
CGTATATTGGGTATATATATGTGTATGCTATAACTAATGGCTATGGAAAGAGAACACTAT
GAAGTATATTATTCAAAATCCCCAAAAAAGAAAGATAATTTTAATATGTATTTAATTTAT
GAATATAAAATATGGTTTTCAAAGAAATTTTAAAATTCATTTCACTAATAAATTATGTAT
TCACAAATGCGGATTATAATCGCAGCGTGTAATGCTGCTACATTACTATGAGAATAACCA
TTTTTTTTATAGCTCTAGTAACTTGGAAAAATATAAAATAAAGTATTCCTTTTAATTTAC
TTAAATATGTATATTTAAATTAATTTTTAATGCTACAATAAAATGGCTTAAGTTTTTTCG
TAGTATATAAAAATTAGCAATTTTAAATGCTATATATATGCGAAAAGGGAAGCATATCAT
CCGAAAATGAAGTGAAAAAATAAATTAGTATGGATAATATATAATAGCAATTTAATAAAA
AGTACAAATGATTATAATAGCAAAAATGTTGCATATTAAAGCATAATTATAAAAATAACC
ACCGAAGCCCCTAATGTAAAATATAAGCACCTTAAATCACATAAAATAAATAATATAGTT
ATTACACCCCTTTCCTTATTTAGCACTTATACAAGATAATTAACGATATATTAAATTTAT
ATATAGTTTTTATTAAAAATATATATTTTATAAAATAACGTGCCTCTAAATAAACAAAAA
ATTATATATATTATTTATGATATGAGACGAATGGATTACCTACTACCCTACTTTATTTCT
GAAAAAAAATAAAAAAAACTAGTAATTTCTTTTAATATCGCCATGCAGCGATATTAGAAT
GCATAAATATTACTATTTATAATGGCAATTGATATAACGTGCACACACATGCAAAACTAA
TACTATGTAAGTGTGATATATGATAACCATTATAGGAATATTACTAATATTGTTTCAAAA
TATAAATGTATTTTATCTTGCAATAAATTATGCATATCCAATATTATTGTATATCATAGG
TGTACATTATTAATATATGTCCCCCCTATATATTACTATTAATTAATTATTTATTTTTAT
CACTTATTGCAAGTTTTCCTGCATATCATCTCTTAATCATATATTGCCAAAAAAATATAT
TTTTTTAAATAACGTCAATATTTCTATAAAAAATATTATAAAAATAATTTAATTTTTAAA
TATTTAAATAAAAAAAAAATATTCTTAATAAATTCTTAAACTTTTATAGTTAAAAATTTT
CCACGTTTTAAAAAACAAAAACTCTCTAATTAATTTAATTAAACAATTTTATAATTAAAA
TTAATTAAAACTTTTATAATATTAAACTTATTTATCTCAAGCAAATATAATATTTTAAAA
AAACATAAAACCATGGGGATCCATGAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCC
AATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGG
TGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACT
ACCTGTTCCTTGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAG
ATACCCAGATCACATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGT
ACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAA
GTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGA
TGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCAT
GGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGA
TGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGT
CCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGA
AAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCAT
GGATGAACTATACAAAGGGATCCTGGCTAGCCAGTCGACCTGCAGGCATGCAAGCTTGCG
GCCGATCCAAATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCGCT
GGAAGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCC
TGGAACAATTGCTTTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGAATACTT
CGAAATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAG
AATCGTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGGGCGCGTTATT
TATCGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAG
TATGAACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTT
GAACGTGCAAAAAAAATTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGA
TTACCAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAA
TGAATACGATTTTGTACCAGAGTCCTTTGATCGTGACAAAACAATTGCACTGATAATGAA
TTCCTCTGGATCTACTGGGTTACCTAAGGGTGTGGCCCTTCCGCATAGAACTGCCTGCGT
CAGATTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGAT
TTTAAGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGAT
ATGTGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCT
TCAGGATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAA
AAGCACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGC
ACCTCTTTCGAAAGAAGTCGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACG
ACAAGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGA
TAAACCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTGTGGATCTGGA
TACCGGGAAAACGCTGGGCGTTAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGAT
TATGTCCGGTTATGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATG
GCTACATTCTGGAGACATAGCTTACTGGGACGAAGACGAACACTTCTTCATAGTTGACCG
CTTGAAGTCTTTAATTAAATACAAAGGATATCAGGTGGCCCCCGCTGAATTGGAATCGAT
ATTGTTACAACACCCCAACATCTTCGACGCGGGCGTGGCAGGTCTTCCCGACGATGACGC
CGGTGAACTTCCCGCCGCCGTTGTTGTTTTGGAGCACGGAAAGACGATGACGGAAAAAGA
GATCGTGGATTACGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCGGAGGAGTTGT
GTTTGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGA
GATCCTCATAAAGGCCAAGAAGGGCGGAAAGATCGCCGTGTAATTCTAGAAGATCCCGTT
TTTCTTACTTATATATTTATACCAATTGATTGTATTTATAACTGTAAAAATGTGTATGTT
GTGTGCATATTTTTTTTTGTGCATGCACATGCATGTAAATAGCTAAAATTATGAACATTT
TATTTTTTGTTCAGAAAAAAAAAACTTTACACACATAAAATGGCTAGTATGAATAGCCAT
ATTTTATATAAATTAAATCCTATGAATTTATGACCATATTAAAAATTTAGATATTTATGG
AACATAATATGTTTGAAACAATAAGACAAAATTATTATTATTATTATTATTTTTACTGTT
ATAATTATGTTGTCTCTTCAATGATTCATAAATAGTTGGACTTGATTTTTAAAATGTTTA
TAATATGATTAGCATAGTTAAATAAAAAAAGTTGAAAAATTAAAAAAAAACATATAAACA
CAAATGATGTTTTTTCCTTCAATTTCGGGTACCGAGCTCGAATTCTCTTGAGCCCGTTAA
TGAAATAGATACAATTCATTCATGTTATATACATCTAGAACATAATCTGAATATGGTTCA
AGTTAAATGTCCAAAAATTATAAAAAGTGATGATATTTTTGATGGTAATACCATAATAGA
CACCAAGGTAACATCACGAAGTAGTCAACAAAATAATTTTTATTTAGAAAATACAGATGT
TGAACCAGAAGAAATAGAGAAATATAAAAATATAGAATACATACCAGAAAACGATGAAGT
AATGCATCTAGACAAAAAAGAAAAGCTAGATGATATATTACCAGGTGTTATCATATTAGA
TAAAAATAAAATGTTCAAAGAAAAAGGACATTTCACTTTTGTTACTCCATTAATTGTAGA
AAAGGTATTAATATTAAAAATATATTGTGATAATACTAAAACAATAATTAATAATATGAA
AGGGAAAAAAGGTATTACAGTAATAAGGATTTCTCAAAATACAACAAAAAATAAATTTTA
TGGATGTGACTTTTCAGGTAATTCTAAAAAAACATTTTACTATTCCAATGTTTATGATTT
AGAAAAAAAAAATGAGTTTTGTGAAATAGAATTAAAAGAAAATATAGTAGTTAGCTTAAA
TTGTCCAACTGGTAAAATTAATCCAAAAAATTGTTTTAGAAATGTATATATAAAAAGTAA
TATGAATGAACAAACAACCGAAAATATAGAAAATATATTTAACGAAATAAAAGTTATAGA
TGCAGATTATTTTATAAATAATTCATCAACCTTTTTGATGATTTCCAAAATTACAAAAAA
AGAGTTTGATTTTTATTGTACATGTGAAGATTATAAAACCAAAAATATAGGAACAATATA
TATTAAAAATTATGAATATCTAGATTCAAAACCTAAATATAAAAATAAACAAATTTCCTA
TATAGATGTAGTTCCATACCCGCGGGGAAAGGGCGAATTCACTGGCCGTCGTTTTACAAC
GTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTT
TCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCA
GCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTT
CACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGC
CCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCG
CTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCAT
CACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCA
TGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCC
CTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCT
GATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCG
CCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGG
TGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATC
TCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCA
CTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAAC
TCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAA
AGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTG
ATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTT
TTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATG
AAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGC
GCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGA
TGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTA
TTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGC
CAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGG
ATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGT
CAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAA
GGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTT
CGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTT
TTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTT
TGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGA
TACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAG
CACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATA
AGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGG
GCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGA
GATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACA
GGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAA
ACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTT
TGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTAC
GGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATT
CTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGA
CCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAATACGCAAACCGCCTC
TCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAG
CGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTT
TACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACA
CAGGAAACAGCTATGACCATGATTACGCCAAGCTTCCGCGGGTATATGGTAAAGAACCTA
CTAACACAATAAAATATTTAAATAATGTATTTCCTATAAATAAATTTACAGATTTATTTT
TTAATACAAAAGATATAGATATACCAGAAATAAATGATCAGTTTAAAGGTTTTAAATTCT
TTATGACATCATTTATAAATCATGGATCATATCCACTAACAATAGAATGTGGTGTAACAA
ATGGTGGAACTAGTTATAAAAGAGCAATTATTTTATTGCATGTTCGAACTGATTTAAAAG
ATAGACCAGTTTCATTTTGTGATTTTCGAAAAGGAGAATTATATAATTATTTGAATGCTT
ATACTGAAGGGGATGTATGCATAATAATTTCCAAATCAAATACAAGTTTTGGTTTTAGAT
GCCCAGTAAATACAAAAAAAATGCCAAAAAATTGTTTTACGCAAGTATATGAAAAAGGGT
ATCTAAATGACGCCAATAAAATTAATACTAAAAATGTTATTAACTATTCATTTGAAAATC
CAGAATATGCGCTAGCTGGTTYTAATTATACATTAACAAAATCGTATCAATTTGAATGTC
ATTGTGTAGATAAAGAAACAGAACAAATTGTAAAAACGGTTTTAGTCAAATATGTAAATG
AAGATGAAATATATGATTATAATGATTTTCCAATGGTGAATCACAAACCTATTATTGCAC
ATCCAAATAAAACACATCAAGCTTGCATGCCTGCAGGTCAACAATAAATAATAAATAAAT
ATTGTGGAAATAAAATAACATATAATTATTTTTAATACATTGATTTCCCTTTTATTTTTT
TAAATTTCATTGATATAAAAATATATAATAATAACATATATGATTTCAAATTAATCTTTT
CAAAAATGGTGTTTATTTTTGTATGTTTGTGTATGAATTAATCACATAACACATCTATTA
AATTGAGTTGGTAATATAGACACAAATAAATATATATATTTTTATAGCTTAAAAGTGTGT
TATGAATATTTTAAGCATATTTTCTTTTTCTTTGGATTGTGTAAAATGAACTCATATAAT
GCGTTTTTTTGTTTTTGTTATTTTGTCATTTTGTTATTTTGCTATTTTATGGATTAATTT
TTGTTTATAAAATGGGAAATAATTTTAACATATTTAAATAAATGGAGAAAAAATATAAAA
TAATTATAAAAAAAAGTTAATACACATTTTTTCCTGTTATAGACCTTATATTTATTTATC
CATATATATATATATATATATATATATATATATATACATACCAAGTGAATTAAGAGGAAA
GCTAATTTATTATTCAGAATAATATATGAACTATATATAATTTTTATTATTTTGGTGTAT
ATTAATCTGTCTATATGCATACATGCAATAATTTATCGACTTATATATCAAATAACATAA
AATAGAAGTGTTTTAAATTATGGATATATGCTCAATATTCATTTTTTTTAATAAGTTAGC
TATATTTAAATTATACATTTTATATATGGTCTCTTTTTTTTTTAAATATTATTTAAGTGA
TCATGAAAATATAAATAATTTTTTTTTATTTAATATCCTTTTGCTTGCATGTGGTAAATG
GAAATTTGGATGTGTTTTGAARGTTCGGATATAGTTGTATGGACATATAATATATTTTGT
GAAAAATTGGTTTTATGTTTATACTTATGCCAATACTTTTTGAGTAAAACAAAGCAAGTG
CTTATAAATAATTAAAGCCAATTTTATAATATATATTTTTTTATTTAATTTGAATTTAGT
AGTATAATTTTTTATGGTAAGTGCTCAAAGAGAGTTGCTTATAAAGTATGGTTTGTTTCT
TTTTCGCCATTTTGAATTACACATTAAAAATATATAGATACATATATTATAATATGAAAT
CATTAATAATTTAGGGAAATTCTACAAATTTAAAAACGAATAAAATAATTGTTTTTCATC
ATGCCATAACACAATATTGATATATACATGTACAAACATTTTTTTTATTTGGAAAATATA
AATTATATAAAAAAAAATGTATAGTATACAAAATGAGCATATTCACACGGGGTGGACGTT
CATTTTTTCATTTTTCCCCTGTTTTTTATGAGTATATGATAAAATTTTATGAACATTTAC
ACAAAATGAAAATGGATATATAGGAAAAATGGAGCGGTATTTCATTTATCTTTGATTGTC
ATTTGGATATTATATTACCYTGGGTAGGCAATTAAAAATGTTAAATAACAATTTAAGGAA
ATTATATTTTATATATTAAAATTAACACTGTATTATATGATTCGCTTATAAAAGCCACTC
TTTCCCCATGCAAAGCTGTTTAATATCAATTTTAACAAATTACACACATGTTAATATATT
TATATATATAATTTATATATTTATAATTTATATATTTATATTTTTATTATTTATATATTT
ATTATTTATTGTGTGTGTCAATTCGGGTAGGATATACCTCTTTTTTATTGTTTAAAGCGA
TTTGTATTCTAAAATATAAAGRATTTGAAAAAGAGAAAGATAGAATATGATCCCATCATA
TATAGCCCTATAATTTTTATTTAGCAGCGAATTAATTTTTCTATTAAGTTTATGTGTAAT
TAAAATAACGGAATATATATAATACAATAAAAAAGTGCATAAATTAAAATTTTTTCAATT
AAATTTTTTTTTTTAAGGGGTTATATAATATTAAATATATAAAATACGATTATATATTTT
TGCTACAATTTTTTATATTAAGATATAAATAGTAAATAAATGGTATTATATGGCATGTAA
TATATAAATTTTTTCCAATTTTTATTTTATATACACTTTTCCTTTTTTTGTCATAAAACT
TAAACAATTTACACATTCATTTTAAAAATTGACTATTTGTTTCAACATTTTTTGAGTTTC
CGTTTTATAATAGTATTTTCATTTGTATATTGCTTATATATATAAATACACACCTAAATG
TTACAAAGGATCAATGCATAAACCGGTGTGTCTGGTCGTCGCGATGACCCCCAAGAGGGG
CATCGGCATCAACAACGGCCTCCCGTGGCCCCACTTGACCACAGATTTCAAACACTTTTC
TCGTGTGACAAAAACGACGCCCGAAGAAGCCAGTCGCCTGAACGGGTGGCTTCCCAGGAA
ATTTGCAAAGACGGGCGACTCTGGACTTCCCTCTCCATCAGTCGGCAAGAGATTCAACGC
CGTTGTCATGGGACGGAAAACCTGGGAAAGCATGCCTCGAAAGTTTAGACCCCTCGTGGA
CAGATTGAACATCGTCGTTTCCTCTTCCCTCAAAGAAGAAGACATTGCGGCGGAGAAGCC
TCAAGCTGAAGGCCAGCAGCGCGTCCGAGTCTGTGCTTCACTCCCAGCAGCTCTCAGCCT
TCTGGAGGAAGAGTACAAGGATTCTGTCGACCAGATTTTTGTCGTGGGAGGAGCGGGACT
GTACGAGGCAGCGCTGTCTCTGGGCGTTGCCTCTCACCTGTACATCACGCGTGTAGCCCG
CGAGTTTCCGTGCGACGTTTTCTTCCCTGCGTTCCCCGGAGATGACATTCTTTCAAACAA
ATCAACTGCTGCGCAGGCTGCAGCTCCTGCCGAGTCTGTGTTCGTTCCCTTTTGTCCGGA
GCTCGGAAGAGAGAAGGACAATGAAGCGACGTATCGACCCATCTTCATTTCCAAGACCTT
CTCAGACAACGGGGTTCCCTACGACTTTGTGGTTC
Restriction sites to linearize plasmid KspI (SacII)
Selectable marker used to select the mutant parasitetgdhfr
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
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Other details transgene
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Promoter
Gene Model of Parasite PBANKA_1326400
Gene Model P. falciparum ortholog PF3D7_1462800
Gene productglyceraldehyde-3-phosphate dehydrogenase
Gene product: Alternative name
Primer information details of the primers used for amplification of the promoter sequence  Click to view information
Primer information details of the primers used for amplification of the promoter sequence  Click to hide information
Sequence Primer 1ATAAGAATGCGGCCGCGTATAAACTGAGTTAAGGGAAGTGG
Additional information primer 12607 (NotI); glyc promoter sense
Sequence Primer 2CATGCCATGGTTTTATGTTTTTTTAAAATATTATATTGCTTG
Additional information primer 22608 (NcoI); glyc promoter antisense
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3'-UTR
Gene Model of Parasite PBANKA_0719300
Gene productbifunctional dihydrofolate reductase-thymidylate synthase, putative
Gene product: Alternative namedhfr/ts
Primer information details of the primers used for amplification the 3'-UTR sequences  Click to view information
Primer information details of the primers used for amplification the 3'-UTR sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Insertion/Replacement locus
Replacement / InsertionReplacement locus
Gene Model of Parasite PBANKA_0306000
Gene product6-cysteine protein
Gene product: Alternative name230p
Primer information details of the primers used for amplification of the target sequences  Click to view information
Primer information details of the primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
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Conceptual design of the database: Dr. C.J. Janse (Leiden Malaria Research Group, Leiden, The Netherlands).
Technical design and realization: S. Mededovic and A. van Wigcheren