RMgmDB - Rodent Malaria genetically modified Parasites

Back to search results

Summary

RMgm-5403

Malaria parasite	P. berghei
Genotype
Disrupted	Gene model (rodent): PBANKA_1425900; Gene model (P.falciparum): PF3D7_0811600; Gene product: protein MAP1, putative (PbMAP1)
Phenotype	Asexual bloodstage;

Last modified: 10 March 2024, 09:19

*RMgm-5403

Successful modification	The parasite was generated by the genetic modification
The mutant contains the following genetic modification(s)	Gene disruption
Reference (PubMed-PMID number)	Reference 1 (PMID number) : 37563696
MR4 number
top of page
Parent parasite used to introduce the genetic modification
Rodent Malaria Parasite	P. berghei
Parent strain/line	P. berghei ANKA
Name parent line/clone	Not applicable
Other information parent line
top of page
The mutant parasite was generated by
Name PI/Researcher	Gao J, Chen Q
Name Group/Department	Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine
Name Institute	Shenyang Agricultural University
City	Shenyang
Country	China
top of page
Name of the mutant parasite
RMgm number	RMgm-5403
Principal name	ΔPbMAP1
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modification	Yes
top of page
Phenotype
Asexual blood stage	Reduced growth/multiplication of blood stages in mice (mice infected with ΔPbMAP1 parasites died 4–10 days later than those infected with wild type).
Gametocyte/Gamete	Not tested
Fertilization and ookinete	Not tested
Oocyst	Not tested
Sporozoite	Not tested
Liver stage	Not tested
Additional remarks phenotype	Mutant/mutation The mutant lacks expression of PbMAP1 Protein (function) A protein of 1303 amino acids, which is predicted to contain a signal peptide at the N-terminus. In the paper evidence is presented that it binds heparin and to mouse erythrocytes and is located in merozoites (on the plasma membrane). Phenotype Reduced growth/multiplication of blood stages in mice (mice infected with ΔPbMAP1 parasites died 4–10 days later than those infected with wild type). Additional information Other mutants

Disrupted: Mutant parasite with a disrupted gene

top of page

Details of the target gene

Gene Model of Rodent Parasite

PBANKA_1425900

Gene Model P. falciparum ortholog

PF3D7_0811600

Gene product

protein MAP1, putative

Gene product: Alternative name

PbMAP1

top of page

Details of the genetic modification

Inducable system used

Additional remarks inducable system

Type of plasmid/construct used

(Linear) plasmid double cross-over

PlasmoGEM (Sanger) construct/vector used

Modified PlasmoGEM construct/vector used

Plasmid/construct map

Plasmid/construct sequence

Restriction sites to linearize plasmid

Partial or complete disruption of the gene

Complete

Additional remarks partial/complete disruption

Selectable marker used to select the mutant parasite

tgdhfr

Promoter of the selectable marker

pbdhfr

Selection (positive) procedure

pyrimethamine

Selection (negative) procedure

Additional remarks genetic modification

The plasmid was constructed using pl0001 (MR4; ATCC,
USA). PbMAP1 5′- and 3′-untranslated regions (UTRs)
were PCR-amplifed as the left or right homologous arms
and inserted respectively upstream and downstream of
the DHFR gene. Te 5′-UTR and 3′-UTR were subcloned
in the pl0001 plasmid using restriction enzyme sites (Apa I/BspD I or BamH I/Not I). The plasmid was digested with Kpn I and Sac II before transfection.

Additional remarks selection procedure

Primer information: Primers used for amplification of the target sequences Click to view information

Primer information: Primers used for amplification of the target sequences Click to hide information

Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6

top of page