| Successful modification | The parasite was generated by the genetic modification |
| The mutant contains the following genetic modification(s) |
Gene tagging
|
| Reference (PubMed-PMID number) |
Not published (yet)
|
| MR4 number |
|
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| Parent parasite used to introduce the genetic modification |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | P. berghei ANKA |
| Name parent line/clone |
Not applicable
|
| Other information parent line | Plasmodium berghei ANKA (MRA 311) was obtained from BEI resources, USA |
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| The mutant parasite was generated by |
| Name PI/Researcher | Mishra A, Mishra S |
| Name Group/Department | Division of Molecular Microbiology and Immunology |
| Name Institute | CSIR-Central Drug Research Institute |
| City | Lucknow |
| Country | India |
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| Name of the mutant parasite |
| RMgm number | RMgm-5400 |
| Principal name | PbAtg7-3XHA-mCherry |
| Alternative name | |
| Standardized name | |
| Is the mutant parasite cloned after genetic modification | Yes |
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| Phenotype |
| Asexual blood stage | mCherry signals showed cytosolic localization of Atg7 in the schizont, ring, and trophozoite stages. |
| Gametocyte/Gamete | Not tested |
| Fertilization and ookinete | Not tested |
| Oocyst | No mCheryy signals in mosquito stages |
| Sporozoite | No mCheryy signals in mosquito stages |
| Liver stage | mCherry signals showed cytosolic localization of Atg7 in 24 and 40 hours liver stages. No signals in 55 hour liver stages. |
| Additional remarks phenotype | Mutant/mutation
The mutant expresses an C-terminal 3xHA-mCherry-tagged version of Atg7.
Published in: bioRxiv preprint doi: https://doi.org/10.1101/2023.08.16.553492
Protein (function)
Autophagy-related E1-like enzyme Atg7.
Atg8, a marker of active autophagy in yeast and mammals, is a small ubiquitin-like molecule, and its conjugation with phosphatidyl ethanolamine (PE) on the autophagosome membrane is essential for cargo selection, membrane tethering, hemifusion, expansion, and closure of autophagosomes. In apicomplexan parasites, Atg8 is conserved along with the intermediate genes required for its conjugation with PE and is specifically localized on the apicoplast membrane. The discrete localization of Atg8 on the apicoplast membrane throughout the parasite life cycle must have a distinct role from its role in autophagy.
In this study evidence is presented for the role of Atg7 in Atg8 conjugation, clearance of micronemes, organelle biogenesis, and expansion of parasites during liver stage development
Phenotype
mCherry signals showed cytosolic localization of Atg7 in the schizont, ring, and trophozoite stages. No mCheryy signals in mosquito stages.
mCherry signals showed cytosolic localization of Atg7 in 24 and 40 hours liver stages. No signals in 55 hour liver stages.
Additional information
Other mutants |
*RMgm-5400
