top of page |
Details of the target gene |
Gene Model of Rodent Parasite |
PBANKA_1432300
|
Gene Model P. falciparum ortholog |
PF3D7_1216600
|
Gene product | cell traversal protein for ookinetes and sporozoites |
Gene product: Alternative name | CelTOS, S4 |
top of page |
Details of the genetic modification |
Short description of the mutation | 'promoter swap mutant': the promoter of CelTOS replaced with the ctrp promoter |
Inducable system used | No |
Short description of the conditional mutagenesis | Not available |
Additional remarks inducable system |
|
Type of plasmid/construct | (Linear) plasmid double cross-over |
PlasmoGEM (Sanger) construct/vector used | No |
Modified PlasmoGEM construct/vector used | No
|
Plasmid/construct map |
|
Plasmid/construct sequence |
|
Restriction sites to linearize plasmid |
|
Selectable marker used to select the mutant parasite | tgdhfr |
Promoter of the selectable marker | eef1a |
Selection (positive) procedure | pyrimethamine |
Selection (negative) procedure | No |
Additional remarks genetic modification | DNA fragments containing approximately 800 bp of the 5′ UTR of the Pb S4/CelTOS gene, approximately 700 bp of the 5’ UTR immediately upstream of the start codon of Pb CTRP gene (CTRP promoter), the entire Pb S4/CelTOS open reading frame with stop codon, and the 3’ UTR of Pb S4/CelTOS were amplified from WT Pb NK65 genomic DNA before cloning into the b3D.DT^H.^D vector (BEI Resources). |
Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences
Primer information: Primers used for amplification of the target sequences
Sequence Primer 1 | |
Additional information primer 1 | |
Sequence Primer 2 | |
Additional information primer 2 | |
Sequence Primer 3 | |
Additional information primer 3 | |
Sequence Primer 4 | |
Additional information primer 4 | |
Sequence Primer 5 | |
Additional information primer 5 | |
Sequence Primer 6 | |
Additional information primer 6 | |
|
|
top of page |