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| Details of the target gene |
| Gene Model of Rodent Parasite |
PBANKA_0603200
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| Gene Model P. falciparum ortholog |
PF3D7_1204300
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| Gene product | eukaryotic translation initiation factor 5A |
| Gene product: Alternative name | EIF5A'EIF-5A |
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| Details of the genetic modification |
| Inducable system used | No |
| Additional remarks inducable system |
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| Type of plasmid/construct used | (Linear) plasmid double cross-over |
| PlasmoGEM (Sanger) construct/vector used | No |
| Modified PlasmoGEM construct/vector used | No
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| Plasmid/construct map |
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| Plasmid/construct sequence |
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| Restriction sites to linearize plasmid |
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| Partial or complete disruption of the gene | Complete |
| Additional remarks partial/complete disruption |
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| Selectable marker used to select the mutant parasite | tgdhfr |
| Promoter of the selectable marker | pbdhfr |
| Selection (positive) procedure | pyrimethamine |
| Selection (negative) procedure | No |
| Additional remarks genetic modification | The unsuccessful attempts to disrupt this gene indicate an essential function during asexual blood stage growth/multiplication.
The novel amino acid hypusine is a post-translational modification that only appears in a single small acidic protein in the eukaryotic initiation factor 5A (EIF-5A). Within two subsequent enzymatic steps, a specific lysine residue is modified by the enzymes deoxyhypusine synthase (DHS, EC 2.5.1.46) and deoxyhypusine hydroxylase (DOHH, EC 1.14.99.29). Hypusination is strictly linked to the polyamine pathway. While in the first step, under DHS catalysis, an aminobutyl moiety from the triamine spermidine is transferred to the e-amino group of a specific lysine in eIF-5A (Lys 50 in EIF-5A), DOHH completes hypusine biosynthesis by hydroxylation of the side chain in 2-position and thus activates eIF-5A in the second step. EIF-5A has a central role in translation elongation facilitating peptide bound formation during translation of polyproline-containing proteins. Findings of a genome-wide analysis of 35 representative organisms from six kingdoms of life, that is, archaebacteria, eubacteria, protista, fungi, plantae, and animalia showed that EIF-5A-dependent expression is strongly associated with specific biological processes like actin/cytoskeletal associated functions, RNA splicing/turnover, DNA binding/transcription, and cell signaling. |
| Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences  Primer information: Primers used for amplification of the target sequences 
| Sequence Primer 1 | |
| Additional information primer 1 | |
| Sequence Primer 2 | |
| Additional information primer 2 | |
| Sequence Primer 3 | |
| Additional information primer 3 | |
| Sequence Primer 4 | |
| Additional information primer 4 | |
| Sequence Primer 5 | |
| Additional information primer 5 | |
| Sequence Primer 6 | |
| Additional information primer 6 | |
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*RMgm-1580