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Details of the target gene |
Gene Model of Rodent Parasite |
PBANKA_0602600
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Gene Model P. falciparum ortholog |
PF3D7_1203600
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Gene product | cytochrome c1 heme lyase, putative |
Gene product: Alternative name | CC1HL |
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Details of the genetic modification |
Inducable system used | No |
Additional remarks inducable system |
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Type of plasmid/construct used | (Linear) plasmid double cross-over |
PlasmoGEM (Sanger) construct/vector used | No |
Modified PlasmoGEM construct/vector used | No
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Plasmid/construct map |
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Plasmid/construct sequence |
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Restriction sites to linearize plasmid |
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Partial or complete disruption of the gene | Complete |
Additional remarks partial/complete disruption |
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Selectable marker used to select the mutant parasite | hdhfr/yfcu |
Promoter of the selectable marker | eef1a |
Selection (positive) procedure | pyrimethamine |
Selection (negative) procedure | No |
Additional remarks genetic modification | The unsuccessful attempts to disrupt this gene indicate an essential function during asexual blood stage growth/multiplication.
Malaria parasites possess a de novo heme synthetic pathway, which is dispensable during blood stage development in the mammalian host. The assembly of the two most important hemeproteins, cytochromes c and c1, is mediated by cytochrome heme lyase enzymes. Plasmodium spp. possess two cytochrome heme lyases encoded by separate genes. In P. berghei these genes are PBANKA_143950 and PBANKA_0602600. Evidence is presented that these genes can only be disrupted when the parasite is complemented with a gene copy of the P. falciparum ortholog (see also RMgm-1575). Evidence is presented that four genes in the de novo heme synthesis pathway (PBANKA_1459200; PBANKA_0608000; PBANKA_1140700; PBANKA_0105900) can be disrupted without complementation (see RMgm-1576 - RMgm-1580). |
Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences
Primer information: Primers used for amplification of the target sequences
Sequence Primer 1 | |
Additional information primer 1 | |
Sequence Primer 2 | |
Additional information primer 2 | |
Sequence Primer 3 | |
Additional information primer 3 | |
Sequence Primer 4 | |
Additional information primer 4 | |
Sequence Primer 5 | |
Additional information primer 5 | |
Sequence Primer 6 | |
Additional information primer 6 | |
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