Back to search resultsSummaryRMgm-1303
|
Successful modification | The parasite was generated by the genetic modification |
The mutant contains the following genetic modification(s) | Gene tagging |
Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 26118838 |
MR4 number | |
top of page | |
Parent parasite used to introduce the genetic modification | |
Rodent Malaria Parasite | P. yoelii |
Parent strain/line | P. y. yoelii 17XNL |
Name parent line/clone | Not applicable |
Other information parent line | |
top of page | |
The mutant parasite was generated by | |
Name PI/Researcher | Groat-Carmona AM; Kappe SH |
Name Group/Department | Seattle Biomedical Research Institute |
Name Institute | Seattle Biomedical Research Institute |
City | Seattle |
Country | USA |
top of page | |
Name of the mutant parasite | |
RMgm number | RMgm-1303 |
Principal name | pblp-myc1; pblp-myc2 |
Alternative name | |
Standardized name | |
Is the mutant parasite cloned after genetic modification | Yes |
top of page | |
Phenotype | |
Asexual blood stage | Expression of PBLP-myc in blood stages. Evidence is presented for a parasite plasma membrane (PPM) location |
Gametocyte/Gamete | Not tested |
Fertilization and ookinete | Not tested |
Oocyst | Expression of PBLP-myc in oocysts. In developing oocysts, PBLP significantly co-localized with the circumsporozoite protein (CSP) However, PBLP showed a unique intracellular localization in salivary gland derived pblp-myc tagged sporozoites, which did not overlap with the ER marker BIP, the micronemal marker TRAP or the rhoptry marker Ron4, although it was distinctly vesicular. |
Sporozoite | Expression of PBLP-myc in sporozoites. In developing oocysts, PBLP significantly co-localized with the circumsporozoite protein (CSP) However, PBLP showed a unique intracellular localization in salivary gland derived pblp-myc tagged sporozoites, which did not overlap with the ER marker BIP, the micronemal marker TRAP or the rhoptry marker Ron4, although it was distinctly vesicular. |
Liver stage | In liver stages, at 24 hpi PBLP co-localized with CSP, which at this point is still found associated with the parasite plasma membrane (PPM) of LS parasites. During later points of LS development, PBLP co-localized with MSP-1 on the LS surface, clearly decorating the plasma membrane invaginations known as cytomeres. In the final stage of exo-erythrocytic schizogony, PBLP continued to co-localize with MSP-1 on the PPM of developed merozoites. Since PBLP was never shown to co-localize with the known parasitophorous vacuole membrane (PVM) protein Hep17, these studies support that PBLP co-localizes with the PPM in blood and liver stages. |
Additional remarks phenotype | Mutant/mutation Additional information See also RMgm-110 for P. berghei mutant lacking expression of Plasmodium BEM46-like protein (PBLP)(PBANKA_071220). This mutant shows a different phenotype as the P. yoelii ko mutant . This P. berghei mutant showed strongly reduced sporozoite formation within oocysts. Sporozoites formed were not infectious to mice. In addition, it showed normal blood stage growth. Other mutants |
top of page | |||||||||||||||||||||||||||
Details of the target gene | |||||||||||||||||||||||||||
Gene Model of Rodent Parasite | PY17X_0712400 | ||||||||||||||||||||||||||
Gene Model P. falciparum ortholog | PF3D7_0818600 | ||||||||||||||||||||||||||
Gene product | BEM46-like protein, putative | ||||||||||||||||||||||||||
Gene product: Alternative name | PBLP | ||||||||||||||||||||||||||
top of page | |||||||||||||||||||||||||||
Details of the genetic modification | |||||||||||||||||||||||||||
Name of the tag | c-myc | ||||||||||||||||||||||||||
Details of tagging | C-terminal | ||||||||||||||||||||||||||
Additional remarks: tagging | quadruple myc tag | ||||||||||||||||||||||||||
Commercial source of tag-antibodies | |||||||||||||||||||||||||||
Type of plasmid/construct | (Linear) plasmid single cross-over | ||||||||||||||||||||||||||
PlasmoGEM (Sanger) construct/vector used | No | ||||||||||||||||||||||||||
Modified PlasmoGEM construct/vector used | No | ||||||||||||||||||||||||||
Plasmid/construct map | |||||||||||||||||||||||||||
Plasmid/construct sequence | |||||||||||||||||||||||||||
Restriction sites to linearize plasmid | |||||||||||||||||||||||||||
Selectable marker used to select the mutant parasite | tgdhfr | ||||||||||||||||||||||||||
Promoter of the selectable marker | pbdhfr | ||||||||||||||||||||||||||
Selection (positive) procedure | pyrimethamine | ||||||||||||||||||||||||||
Selection (negative) procedure | No | ||||||||||||||||||||||||||
Additional remarks genetic modification | |||||||||||||||||||||||||||
Additional remarks selection procedure | |||||||||||||||||||||||||||
| |||||||||||||||||||||||||||
top of page |