RMgmDB - Rodent Malaria genetically modified Parasites
Back to search resultsSummaryRMgm-1200
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*RMgm-1200| Successful modification | The parasite was generated by the genetic modification |
| The mutant contains the following genetic modification(s) | Other |
| Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 25732065 |
| MR4 number | |
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| Parent parasite used to introduce the genetic modification | |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | P. berghei ANKA |
| Name parent line/clone | Not applicable |
| Other information parent line | |
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| The mutant parasite was generated by | |
| Name PI/Researcher | Gomes AR; Billker O |
| Name Group/Department | Wellcome Trust Sanger Institute |
| Name Institute | Wellcome Trust Sanger Institute |
| City | Hinxton Cambridge |
| Country | UK |
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| Name of the mutant parasite | |
| RMgm number | RMgm-1200 |
| Principal name | - |
| Alternative name | |
| Standardized name | |
| Is the mutant parasite cloned after genetic modification | No |
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| Phenotype | |
| Asexual blood stage | Not different from wild type |
| Gametocyte/Gamete | Not tested |
| Fertilization and ookinete | Not tested |
| Oocyst | Not tested |
| Sporozoite | Not tested |
| Liver stage | Not tested |
| Additional remarks phenotype | Successful gene deletion as determined by barcode PCR in a large pool of gene-deletion mutants. No evidence was found for reduced fitness (growth rate) of blood stages In this study P. berghei blood stages were transfected with a large pool of barcoded disruption (gene-deletion) vectors. These disruption vectors contained long modification arms to efficiently target the genes of interest. To analyze growth curves derived from barcode counting two parameters were considered: (1) the relative abundance of each barcode within the pool, and (2) the relative fitness of each mutant, i.e., the rate at which its abundance changed each day (i.e. growth rate). |
Other: Mutant parasite with another genetic modification| top of page | |
| Details of the target gene | |
| Gene Model of Rodent Parasite | PBANKA_0615200 |
| Gene Model P. falciparum ortholog | PF3D7_0717500 |
| Gene product | calcium-dependent protein kinase 4 |
| Gene product: Alternative name | CDPK4 |
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| Description | |
| Short description of the modification | Successful gene deletion as determined by barcode PCR in a large pool of gene-deletion mutants |
| Description | In this study P. berghei blood stages were transfected with a large pool of barcoded disruption (gene-deletion) vectors. These disruption vectors contained long modification arms to efficiently target the genes of interest. In addition the vectors contain gene-specific molecular barcodes. Co-transfecting multiple gene-deletion vectors in the same electroporation reproducibly generates complex pools of barcoded P. berghei mutants. In this study parasites were (mainly) transfected with a pool of vectors targeting protein kinase genes for deletion. Unsuccessful gene disruption/deletion or successful gene disruption/deletion is determined by the absence or presence of the barcode in the population (as determined by barcode-specific PCR analysis). The gene was targeted by PlasmoGEM vector PbGEM-087803 |
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