Summary

RMgm-1192
Malaria parasiteP. berghei
Genotype
Genetic modification not successful
Other modificationGene model (rodent): PBANKA_1126900; Gene model (P.falciparum): PF3D7_0628200; Gene product: protein kinase PK4|eukaryotic translation initiation factor 2-alpha kinase (PK4; PERK)
Details modification: Unsuccessful gene deletion as determined by barcode PCR in a large pool of gene-deletion mutants
PhenotypeNo phenotype has been described
Last modified: 21 March 2015, 19:51
  *RMgm-1192
Successful modificationThe gene/parasite could not be changed/generated by the genetic modification.
The following genetic modifications were attempted Other
Number of attempts to introduce the genetic modification 2
Reference (PubMed-PMID number) Reference 1 (PMID number) : 25732065
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone Not applicable
Other information parent line
Attempts to generate the mutant parasite were performed by
Name PI/ResearcherGomes AR; Billker O
Name Group/DepartmentWellcome Trust Sanger Institute
Name InstituteWellcome Trust Sanger Institute
CityHinxton Cambridge
CountryUK

  Other: Mutant parasite with another genetic modification
Details of the target gene
Gene Model of Rodent Parasite PBANKA_1126900
Gene Model P. falciparum ortholog PF3D7_0628200
Gene productprotein kinase PK4|eukaryotic translation initiation factor 2-alpha kinase
Gene product: Alternative namePK4; PERK
Description
Short description of the modificationUnsuccessful gene deletion as determined by barcode PCR in a large pool of gene-deletion mutants
DescriptionIn this study P. berghei blood stages were transfected with a large pool of barcoded disruption (gene-deletion) vectors. These disruption vectors contained long modification arms to efficiently target the genes of interest.
In addition the vectors contain gene-specific molecular barcodes. Co-transfecting multiple gene-deletion vectors in the same electroporation reproducibly generates complex pools of barcoded P. berghei mutants.
In this study parasites were (mainly) transfected with a pool of vectors targeting protein kinase genes for deletion.
Unsccessful gene disruption/deletion or successful gene disruption/deletion is determined by the absence or presence of the barcode in the population (as determined by barcode-specific PCR analysis).

The gene was targeted by PlasmoGEM vector PbGEM-099789