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Details of the target gene |
Gene Model of Rodent Parasite |
PY17X_1339000
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Gene Model P. falciparum ortholog |
PF3D7_1471100
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Gene product | exported protein 2 |
Gene product: Alternative name | EXP2 |
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Details of the genetic modification |
Name of the tag | triple-HA |
Details of tagging | C-terminal |
Additional remarks: tagging | |
Commercial source of tag-antibodies | |
Type of plasmid/construct | (Linear) plasmid single cross-over |
PlasmoGEM (Sanger) construct/vector used | No |
Modified PlasmoGEM construct/vector used | No
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Plasmid/construct map |
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Plasmid/construct sequence |
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Restriction sites to linearize plasmid |
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Selectable marker used to select the mutant parasite | tgdhfr |
Promoter of the selectable marker | pbdhfr |
Selection (positive) procedure | pyrimethamine |
Selection (negative) procedure | No |
Additional remarks genetic modification | To tag the pyexp2 gene, a single cross-over recombination strategy was used. The pSE02-3HA plasmid containing pyrimethamine resistant Tg-dhfr-ts as a selectable marker was used to generate the tagging construct. A 495 bp fragment from the 3’ end of the pyexp2 gene was amplified from P. yoelii 17X genomic DNA and cloned into pSE02-3HA preceding the triple HA sequence resulting in the construct pSE02-3’Exp2-3HA. Plasmid DNA was isolated as above and linearized by digestion with EcoRI prior to transfection. |
Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences
Primer information: Primers used for amplification of the target sequences
Sequence Primer 1 | |
Additional information primer 1 | |
Sequence Primer 2 | |
Additional information primer 2 | |
Sequence Primer 3 | |
Additional information primer 3 | |
Sequence Primer 4 | |
Additional information primer 4 | |
Sequence Primer 5 | |
Additional information primer 5 | |
Sequence Primer 6 | |
Additional information primer 6 | |
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