| Successful modification | The parasite was generated by the genetic modification |
| The mutant contains the following genetic modification(s) |
Gene disruption
|
| Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 24657782
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| MR4 number |
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| Parent parasite used to introduce the genetic modification |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | P. berghei ANKA |
| Name parent line/clone |
Not applicable
|
| Other information parent line | |
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| The mutant parasite was generated by |
| Name PI/Researcher | Offeddu, V; Matuschewski, K. |
| Name Group/Department | Parasitology Unit |
| Name Institute | Max Planck Institute for Infection Biology |
| City | Berlin |
| Country | Germany |
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| Name of the mutant parasite |
| RMgm number | RMgm-1001 |
| Principal name | p113(-) |
| Alternative name | |
| Standardized name | |
| Is the mutant parasite cloned after genetic modification | Yes |
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| Phenotype |
| Asexual blood stage | Not different from wild type |
| Gametocyte/Gamete | Not tested |
| Fertilization and ookinete | Not tested |
| Oocyst | Not different from wild type |
| Sporozoite | Not different from wild type |
| Liver stage | Sporozoites show normal gliding motility, attachment to hepatocytes and invasion of hepatocytes (possibly an enhanced invasion rate). Reduced liver stage development as shown by a prolonged pre-patent period (possibly due to reduced transformation of intracellular sporozoites into early liver stages and reduced production of merozoites). |
| Additional remarks phenotype | Mutant/mutation
The mutant lacks expression of P113.
Protein (function)
P113 has a putative GPI anchor and amino terminal signal peptide. The P. falciparum ortholog has been isolated from blood stages in raft-like, detergent-resistant membranes. PfP113 was also detected in gametocytes and sporozoites.
Phenotype
Only clones of a single mutant have been analysed. Normal blood stage and mosquito stage development. Sporozoites show normal gliding motility, attachment to hepatocytes and invasion of hepatocytes (possibly an enhanced invasion rate). Reduced liver stage development as shown by a prolonged pre-patent period (possibly due to reduced transformation of intracellular sporozoites into early liver stages and reduced production of merozoites).
Additional information
Expression (analysed by RT-qPCR) showed expression on schizonts and slivary gland sporozoites (upregulated in comparison with midgut-sporozoites) and in (all) liver stages.
Other mutants |
*RMgm-1001
