RMgmDB - Rodent Malaria genetically modified Parasites
Back to search resultsSummaryRMgm-971
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*RMgm-971| Successful modification | The parasite was generated by the genetic modification |
| The mutant contains the following genetic modification(s) | Gene tagging |
| Reference (PubMed-PMID number) | Not published (yet) |
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| Parent parasite used to introduce the genetic modification | |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | P. berghei ANKA |
| Name parent line/clone | P. berghei ANKA 2.34 |
| Other information parent line | P. berghei ANKA 2.34 is a cloned, gametocyte producer line of the ANKA strain (PubMed: PMID: 15137943). |
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| The mutant parasite was generated by | |
| Name PI/Researcher | Siden-Kiamos, I. |
| Name Group/Department | Institute of Molecular Biology and Biotechnology |
| Name Institute | FORTH |
| City | Heraklion |
| Country | Greece |
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| Name of the mutant parasite | |
| RMgm number | RMgm-971 |
| Principal name | 100390-HA |
| Alternative name | |
| Standardized name | |
| Is the mutant parasite cloned after genetic modification | No |
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| Phenotype | |
| Asexual blood stage | Not different from wild type |
| Gametocyte/Gamete | Not different from wild type |
| Fertilization and ookinete | Not different from wild type |
| Oocyst | Not tested |
| Sporozoite | Not tested |
| Liver stage | Not tested |
| Additional remarks phenotype | Mutant/mutation The phenotype has not been analysed in detail. No tagged-protein could be detected by immuno-fluorescence analyses in asexual blood stages, gametocytes and ookinetes. An additional mutant has been generated that contains the PBANKA_1003900 gene (C-terminally) tagged with GFP on episomes. The tagged-gene is under the control of ~1kb 5'UTR region of PBANKA_1003900 and the 3'UTR of the pbdhfr/ts gene. Also in this mutant no tagged-protein could be detected in asexual blood stages, gametocytes and ookinetes. The similarity of P. falciparum Pfs16 and the syntenic P. berghei gene (Pbs16?) is below threshold. See also RMgm-968 for a mutant with a disrupted PBANKA_100390) gene locus
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Tagged: Mutant parasite with a tagged gene| top of page | |||||||||||||||||||||||||||
| Details of the target gene | |||||||||||||||||||||||||||
| Gene Model of Rodent Parasite | PBANKA_1003900 | ||||||||||||||||||||||||||
| Gene Model P. falciparum ortholog | PF3D7_0406200 | ||||||||||||||||||||||||||
| Gene product | sexual stage-specific protein precursor | ||||||||||||||||||||||||||
| Gene product: Alternative name | Pfs16 | ||||||||||||||||||||||||||
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| Details of the genetic modification | |||||||||||||||||||||||||||
| Name of the tag | triple-HA | ||||||||||||||||||||||||||
| Details of tagging | C-terminal | ||||||||||||||||||||||||||
| Additional remarks: tagging | The PBANKA_100390 gene has been tagged using a PlasoGem DNA-construct See the PlasmoGem website for details of the DNA construct | ||||||||||||||||||||||||||
| Commercial source of tag-antibodies | |||||||||||||||||||||||||||
| Type of plasmid/construct | (Linear) plasmid double cross-over | ||||||||||||||||||||||||||
| PlasmoGEM (Sanger) construct/vector used | Yes | ||||||||||||||||||||||||||
| Name of PlasmoGEM construct/vector | 95547 | ||||||||||||||||||||||||||
| Modified PlasmoGEM construct/vector used | No | ||||||||||||||||||||||||||
| Plasmid/construct map | |||||||||||||||||||||||||||
| Plasmid/construct sequence | |||||||||||||||||||||||||||
| Restriction sites to linearize plasmid | |||||||||||||||||||||||||||
| Selectable marker used to select the mutant parasite | hdhfr/yfcu | ||||||||||||||||||||||||||
| Promoter of the selectable marker | eef1a | ||||||||||||||||||||||||||
| Selection (positive) procedure | pyrimethamine | ||||||||||||||||||||||||||
| Selection (negative) procedure | No | ||||||||||||||||||||||||||
| Additional remarks genetic modification | |||||||||||||||||||||||||||
| Additional remarks selection procedure | |||||||||||||||||||||||||||
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StudioDrie; S. Mededovic and A. van Wigcheren