RMgmDB - Rodent Malaria genetically modified Parasites


Malaria parasiteP. berghei
DisruptedGene model (rodent): PBANKA_0314200; Gene model (P.falciparum): PF3D7_0217500; Gene product: calcium-dependent protein kinase 1 (cdpk1)
Phenotype Oocyst;
Last modified: 24 November 2013, 13:27
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene disruption
Reference (PubMed-PMID number) Reference 1 (PMID number) : 24265753
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone Not applicable
Other information parent line
The mutant parasite was generated by
Name PI/ResearcherS. Jebiwott, P. Bhanot
Name Group/DepartmentDepartment of Microbiology and Molecular Genetics
Name InstituteRutgers New Jersey Medical School, Rutgers, The State University of New Jersey
CityNewark, New Jersey
Name of the mutant parasite
RMgm numberRMgm-966
Principal nameCDPK1-
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationYes
Asexual blood stageNot different from wild type
Gametocyte/GameteNot tested
Fertilization and ookineteNot tested
OocystNo oocyst formation
SporozoiteNot tested
Liver stageNot tested
Additional remarks phenotype

The mutant lacks expression of CDPK1

Protein (function)
In malaria parasites, intracellular Ca2+ signals are translated into stage-specific cellular responses by members of a family of Ca2+-dependent protein kinases (CDPKs), which combine an N-terminal kinase domain with a C-terminal, calmodulin-like regulatory domain in the same polypeptide. By analysis of a promoter-swap mutant that expresses CDPK1 only in asexual blood stages and not in gametocytes and ookinetes it has been shown that CDPK1 plays an essential role in ookinete formation (see RMgm-773)

The phenotype analyses indicate that CDPk1 is not essential for asexual blood stage growth/multiplication. See also RMgm-518 for unsuccessful attempts to disrupt P. berghei cdpk1, suggesting an essential role of CDPK1 for asexual blood stages.
The course of infection in mice of the CDPK- mutant was similar to that of wild type parasites. No analyses are provided for gametocyte and ookinete production. The mutant did not produce oocysts in mosquitoes which is in agreement with the previous reported essential role of CDPK1 for formation of ookinetes (see RMgm-773)

Additional information

Other mutants
RMgm-518: unsuccessful attempts to disrupt cdpk1
RMgm-773: A promoter exchange mutant that expresses CDPK1 under control of the the asexual blood stage specific clag promoter (analysis of this mutant indicate an essential role of CDPK1 for ookinete development)
RMgm-772: A mutant expressing CDPK1-GFP from the endogenous cdpk1 promoter
RMgm-967: A mutant containing a FRTed ORF of the cdpk1 locus. Analyses of this mutant indicates the lack of an essential role of CDPK1 for liver stage development

  Disrupted: Mutant parasite with a disrupted gene
Details of the target gene
Gene Model of Rodent Parasite PBANKA_0314200
Gene Model P. falciparum ortholog PF3D7_0217500
Gene productcalcium-dependent protein kinase 1
Gene product: Alternative namecdpk1
Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct usedPlasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Partial or complete disruption of the geneComplete
Additional remarks partial/complete disruption
Selectable marker used to select the mutant parasitetgdhfr
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Additional information primer 1
Additional information primer 2
Additional information primer 3
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6