RMgmDB - Rodent Malaria genetically modified Parasites

Summary

RMgm-955
Malaria parasiteP. berghei
Genotype
TaggedGene model (rodent): PBANKA_1447800; Gene model (P.falciparum): PF3D7_1233100; Gene product: conserved protein, unknown function
Name tag: GFP-4xMyc
PhenotypeNo phenotype has been described
Last modified: 20 November 2013, 19:23
  *RMgm-955
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene tagging
Reference (PubMed-PMID number) Not published (yet)
MR4 number
top of page
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone P. berghei ANKA cl15cy1
Other information parent lineA reference wild type clone from the ANKA strain of P. berghei (PubMed: PMID: 17406255).
top of page
The mutant parasite was generated by
Name PI/ResearcherKooij, T.W.A.
Name Group/DepartmentParasitology Unit
Name InstituteMax Planck Institute for Infection Biology
CityBerlin
CountryGermany
top of page
Name of the mutant parasite
RMgm numberRMgm-955
Principal nameDUF-GMp
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationNo
top of page
Phenotype
Asexual blood stageNot tested
Gametocyte/GameteNot tested
Fertilization and ookineteNot tested
OocystNot tested
SporozoiteNot tested
Liver stageNot tested
Additional remarks phenotype

Mutant/mutation
The mutant expresses a C-terminal GFP/c-myc (4x)-tagged version of PBANKA_144780 (DUF1754).

This mutant has not been cloned and not analysed in detail.

Protein (function)

Phenotype

Additional information

Other mutants
See RMgm-941 for unsuccessful attempts to disrupt this gene (DUF1754) indicating an essential function during asexual blood stage growth
RMgm-954: A mutant expressing a C-terminal c-myc (4x)-tagged version of PBANKA_144780 (DUF1754).

  Tagged: Mutant parasite with a tagged gene
top of page
Details of the target gene
Gene Model of Rodent Parasite PBANKA_1447800
Gene Model P. falciparum ortholog PF3D7_1233100
Gene productconserved protein, unknown function
Gene product: Alternative name
top of page
Details of the genetic modification
Name of the tagGFP-4xMyc
Details of taggingC-terminal
Additional remarks: tagging
Commercial source of tag-antibodies
Type of plasmid/constructPlasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Selectable marker used to select the mutant parasitehdhfr/yfcu
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6
top of page
Conceptual design of the database: Dr. C.J. Janse (Leiden Malaria Research Group, Leiden, The Netherlands).
Technical design and realization: S. Mededovic and A. van Wigcheren