RMgmDB - Rodent Malaria genetically modified Parasites

Summary

RMgm-941
Malaria parasiteP. berghei
Genotype
Genetic modification not successful
DisruptedGene model (rodent): PBANKA_1447800; Gene model (P.falciparum): PF3D7_1233100; Gene product: conserved protein, unknown function
PhenotypeNo phenotype has been described
Last modified: 20 November 2013, 19:06
  *RMgm-941
Successful modificationThe gene/parasite could not be changed/generated by the genetic modification.
The following genetic modifications were attempted Gene disruption
Number of attempts to introduce the genetic modification 2
Reference (PubMed-PMID number) Not published (yet)
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Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone P. berghei ANKA cl15cy1
Other information parent lineA reference wild type clone from the ANKA strain of P. berghei (PubMed: PMID: 17406255).
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Attempts to generate the mutant parasite were performed by
Name PI/ResearcherKooij, T.W.A.
Name Group/DepartmentParasitology Unit
Name InstituteMax Planck Institute for Infection Biology
CityBerlin
CountryGermany
  Disrupted: Mutant parasite with a disrupted gene
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Details of the target gene
Gene Model of Rodent Parasite PBANKA_1447800
Gene Model P. falciparum ortholog PF3D7_1233100
Gene productconserved protein, unknown function
Gene product: Alternative name
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Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct usedPlasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Partial or complete disruption of the geneComplete
Additional remarks partial/complete disruption
Selectable marker used to select the mutant parasitehdhfr/yfcu
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modificationThe unsuccessful attempts to disrupt this gene (DUF1754) indicate an essential function during asexual blood stage growth.
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1AAACCGCGGAATGTTTGATGCCTCTCTGC
Additional information primer 1Pb0200
Sequence Primer 2AAAGAAAGAATTCCATAGACAAAAAAAAAAACCCAAAATATGTCC
Additional information primer 2Pb0201
Sequence Primer 3AAAGGGCCCATTTTTCTATATCTGAAATCAGTTTGC
Additional information primer 3Pb0204
Sequence Primer 4AAAGGTACCTGAATTATAGATGTACAAAGCAAAGG
Additional information primer 4Pb0205
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6
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Conceptual design of the database: Dr. C.J. Janse (Leiden Malaria Research Group, Leiden, The Netherlands).
Technical design and realization: S. Mededovic and A. van Wigcheren