Mutant/mutation
Two mutants have been generated and analysed that express OVA:
1) PbA-hsOVA expresses a recombinant fusion protein containing the N-terminal sequence (aa 1–5) of P. berghei hsp70, an OVA323-339 MHC II epitope, a truncated sequence (aa 201–398) of P. yoelii hsp70, and an OVA257-264 MHC I epitope.
2) PbA-gfpOVA express a protein containing an OVA257-264 MHC I epitope fused to the C-terminus of GFP

These mutants have been generated as described for mutant RMgm-281 but no details are provided for the promoter used to drive transgene expression,  selectable marker, insertion locus etc.

Protein (function)

Phenotype
PbA-gfpOVA expressed in the parasitophorous vacuole (membrane) of blood stages and liver stages. PbA-gfpOVA expressed expressed in the cytoplasm of sporozoites.

Additional information
Evidence is presented that the ovalbumin epitope is presented by infected liver cells in a transporter associated with antigen processing–dependent manner and becomes a target of specific CD8+ T cells (OT-I cells), leading to protection at the liver stage of Plasmodium infection. The interaction between OT-I cells and infected hepatocytes was visualized by intravital imaging using two-photon microscopy. OT-I cells formed clusters around infected hepatocytes, leading to the elimination of the intra-hepatic parasites and subsequent formation of large clusters of OT-I cells in the liver.

Other mutants