Additional remarks phenotype | Mutant/mutation
The mutant expresses a C-terminal mCherry tagged version of SEP2. The tagged version is integrated into the c/d-ssurRNA gene locus.
Protein (function)
Early transcribed membrane protein (ETRAMP) family member. Plasmodium conserved family with greater than ten members in P. falciparum. ETRAMPs are abundantly expressed early in the intraerythrocytic cycle and are small (frequently less than 200 aa) integral membrane proteins that are localized within the parasitophorous vacuolar membrane (PVM). All members have signal peptides plus a transmembrane domain. The ETRAMP/SEP proteins of P. yoelii and P. berghei (8-11 genes) show homology to members of the ETRAMP family of proteins of P. falciparum (14 genes) but the orthologous relationship of the different members is not completely resolved.
P. berghei sep genes, Pbsep1, Pbsep2 and Pbsep3 (PBANKA_052480, PBANKA_052420 and PBANKA_050110, respectively) are 3 ETRAMP members which reside in the subtelomeric regions of chromosome 5. The three genes share the upstream regulatory region and differ in their 3’UTRs. The encoded proteins (13-16 KDa) are nearly identical in the first 81 amino acids, which include a predicted signal peptide, a lysine-rich domain and a TM region, while differ in their C-terminal portion.
Mutants lacking expression of SEP1 have been generated (RMgm-16, RMgm-18) which show a normal phenotype during the complete life cycle, comparable to wild type parasites.
Multiple unsuccessful attempts to disrupt sep2 en sep3 (RMgm-64, RMgm-65) indicate that these proteins are essential for blood stage proliferation.
In asexual blood stages SEP2 and SEP3 localize to the PVM and to vesicle-like structures exported to the erythrocyte cytosol (RMgm-680, RMgm-681), while SEP1 is mainly confined to the PVM.
Phenotype:
SEP2 is expressed in all life cycle stages. Evidence is provided for a PVM location in blood stages and liver stages and for a surface location in ookinetes and sporozoites. SEP2 is shed/released during gliding motility of sporozoites
Specific antibodies detect SEP2 in dot-like structures inside the ookinete but also close to the parasite periphery, as shown by partial co-localization with the ookinete surface protein P28. SEP2 does not co-localize with the micronemal protein SOAP
Additional information
In order to establish whether the downstream regulatory regions have a role in the control of sep2 and sep3 expression level of these genes, A. stephensi mosquitoes were infected with two transgenic lines, gfp-3'utr-sep2 and gfp-3'utrsep3, containing a stably integrated gfp reporter under the control of the 1.2-kb upstream regulatory region common to sep genes and the 3'UTR specific for either sep2 or sep3. In asexual blood stages these transgenic lines express GFP at a comparable level, indicating that the specific 3'UTRs have no significant regulatory role. In the gfp-3'utr-sep2 line, GFP fluorescence was clearly detected during oocyst maturation (8, 11, 13 days after the blood meal) with an increased intensity in mature oocysts (13 days). A strong GFP-specific signal was also detected in salivary gland sporozoites (22 days). When the GFP was expressed under the control of the 3'-UTR specific for sep3 (gfp-3'utr-sep3 line), only mature oocysts and salivary gland sporozoites displayed a faint fluorescence. These results show that the 3'UTRs of sep2 and sep3 modulate the expression level of GFP.
Other mutants
Mutants lacking expression of SEP1 (RMgm-16, RMgm-18)
Multiple unsuccessful attempts to disrupt sep2 en sep3 (RMgm-64, RMgm-65)
Mutants expressing a flag-tagged version of SEP2 and SEP3 (RMgm-680, RMgm-681)
A mutant expressing an mCherry-tagged version of SEP3 (RMgm-912) |