Successful modification | The parasite was generated by the genetic modification |
The mutant contains the following genetic modification(s) |
Gene disruption
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Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 23587146 |
MR4 number |
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Parent parasite used to introduce the genetic modification |
Rodent Malaria Parasite | P. yoelii |
Parent strain/line | P. y. yoelii 17XNL |
Name parent line/clone |
Not applicable
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Other information parent line | |
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The mutant parasite was generated by |
Name PI/Researcher | Kangwanrangsan, N; Ishino, T. |
Name Group/Department | Department of Molecular Parasitology |
Name Institute | Graduate School of Medicine, Ehime University |
City | Shitsukawa, Toon, Matsuyama, Ehime |
Country | Japan |
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Name of the mutant parasite |
RMgm number | RMgm-859 |
Principal name | pycpw-wpc-1(−) |
Alternative name | |
Standardized name | |
Is the mutant parasite cloned after genetic modification | Yes |
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Phenotype |
Asexual blood stage | Not different from wild type |
Gametocyte/Gamete | Not different from wild type |
Fertilization and ookinete | Not different from wild type |
Oocyst | Not different from wild type |
Sporozoite | Not different from wild type |
Liver stage | Not different from wild type |
Additional remarks phenotype | Mutant/mutation
The mutant lacks expression of PyCPW-WPC-1 (CPW-WPC family protein).
Protein (function)
PyCPW-WPC-1 belongs to a conserved small family (~8 members) named CPW-WPC family (named after the unique WxC motif found at the end of repeated domains). The members share a similar structure, including a signal peptide and five repeated CPW-WPC domains with four to six cysteine residues.
Phenotype
The phenotype analyses indicate that PyCPW-WPC-1 is not essential during the complete life cycle.
Additional information
Evidence is presented for transcription in gametocytes (highly expressed until zygote formation, with a subsequent decrease in expression).
Evidence is presented that PyCPW-WPC-1 protein is produced exclusively in ookinetes. The expression of PyCPW-WPC-1 protein became prominent at 1 and 4 h of ookinete culture and decreased gradually until 24 h of ookinete maturation.
Immunofluorescent staining using mouse anti-PyCPW-WPC-1 antiserum revealed a strong signal on the surface of zygotes, retorts, and mature ookinetes, but not on gametocytes. The staining pattern was similar to that of Pys25, a well-known ookinete surface protein. In addition, another family member, Py03515, also showed a similar surface localization pattern.
PyCPW-WPC-1 is orthologous to P. berghei PBANKA_135250
Other mutants |