| Successful modification | The parasite was generated by the genetic modification |
| The mutant contains the following genetic modification(s) |
Gene disruption
|
| Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 23587146
|
| MR4 number |
|
| top of page |
| Parent parasite used to introduce the genetic modification |
| Rodent Malaria Parasite | P. yoelii |
| Parent strain/line | P. y. yoelii 17XNL |
| Name parent line/clone |
Not applicable
|
| Other information parent line | |
| top of page |
| The mutant parasite was generated by |
| Name PI/Researcher | Kangwanrangsan, N; Ishino, T. |
| Name Group/Department | Department of Molecular Parasitology |
| Name Institute | Graduate School of Medicine, Ehime University |
| City | Shitsukawa, Toon, Matsuyama, Ehime |
| Country | Japan |
| top of page |
| Name of the mutant parasite |
| RMgm number | RMgm-859 |
| Principal name | pycpw-wpc-1(−) |
| Alternative name | |
| Standardized name | |
| Is the mutant parasite cloned after genetic modification | Yes |
| top of page |
| Phenotype |
| Asexual blood stage | Not different from wild type |
| Gametocyte/Gamete | Not different from wild type |
| Fertilization and ookinete | Not different from wild type |
| Oocyst | Not different from wild type |
| Sporozoite | Not different from wild type |
| Liver stage | Not different from wild type |
| Additional remarks phenotype | Mutant/mutation
The mutant lacks expression of PyCPW-WPC-1 (CPW-WPC family protein).
Protein (function)
PyCPW-WPC-1 belongs to a conserved small family (~8 members) named CPW-WPC family (named after the unique WxC motif found at the end of repeated domains). The members share a similar structure, including a signal peptide and five repeated CPW-WPC domains with four to six cysteine residues.
Phenotype
The phenotype analyses indicate that PyCPW-WPC-1 is not essential during the complete life cycle.
Additional information
Evidence is presented for transcription in gametocytes (highly expressed until zygote formation, with a subsequent decrease in expression).
Evidence is presented that PyCPW-WPC-1 protein is produced exclusively in ookinetes. The expression of PyCPW-WPC-1 protein became prominent at 1 and 4 h of ookinete culture and decreased gradually until 24 h of ookinete maturation.
Immunofluorescent staining using mouse anti-PyCPW-WPC-1 antiserum revealed a strong signal on the surface of zygotes, retorts, and mature ookinetes, but not on gametocytes. The staining pattern was similar to that of Pys25, a well-known ookinete surface protein. In addition, another family member, Py03515, also showed a similar surface localization pattern.
PyCPW-WPC-1 is orthologous to P. berghei PBANKA_135250
Other mutants |
*RMgm-859
StudioDrie; S. Mededovic and A. van Wigcheren