| Successful modification | The parasite was generated by the genetic modification |
| The mutant contains the following genetic modification(s) |
Gene tagging
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| Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 23434509
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| MR4 number |
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| Parent parasite used to introduce the genetic modification |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | P. berghei ANKA |
| Name parent line/clone |
P. berghei ANKA 2.34
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| Other information parent line | P. berghei ANKA 2.34 is a cloned, gametocyte producer line of the ANKA strain (PubMed: PMID: 15137943) |
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| The mutant parasite was generated by |
| Name PI/Researcher | Patzewitz EM; Tewari R |
| Name Group/Department | Centre for Genetics and Genomics, School of Biology, Queens Medical Centre |
| Name Institute | University of Nottingham |
| City | Nottingham |
| Country | UK |
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| Name of the mutant parasite |
| RMgm number | RMgm-841 |
| Principal name | SHLP1-GFP |
| Alternative name | |
| Standardized name | |
| Is the mutant parasite cloned after genetic modification | Yes |
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| Phenotype |
| Asexual blood stage | SHLP1-GFP expression in asexual blood stages |
| Gametocyte/Gamete | SHLP1-GFP expression in gametocytes (prominent expression in male gametes) |
| Fertilization and ookinete | SHLP1-GFP expression in ookinetes |
| Oocyst | (Prominent) SHLP1-GFP expression in oocysts |
| Sporozoite | SHLP1-GFP expression in sporozoites |
| Liver stage | Not tested |
| Additional remarks phenotype | Mutant/mutation
The mutant expresses a C-terminal GFP-tagged version of shewanella-like protein phosphatase 1 (SHLP1).
Protein (function)
Bioinformatic analysis of the Plasmodium genome has revealed the presence of non-conventional protein phosphatases (PPs) containing kelch-like motifs as found in plant PPs and two bacterial Shewanella-like PPs (Shelphs or SHLP), both lacking orthologs in humans. SHLPs are related to the phosphoprotein phosphatase (PPP) family of serine/threonine PPs (STPs), span the eukaryote-prokaryote boundary, and are also present in plants, heterokonts, fungi, and some Protozoa.
Phenotype analyses of a mutant lacking expression of SHLP1 (RMgm-840) indicate a role of this protein in ookinete formation.
Phenotype
Phenotype analyses of a mutant lacking expression of SHLP1 (RMgm-840) indicate a role of this protein in ookinete formation.
Analysis of the mutant expressing the GFP-tagged version of SHLP1showed diffuse fluorescence staining in all parasite stages examined by microscopy with prominent staining in the asexual stages, the male gamete, and oocyst. Despite the presence of a predicted apicoplast targeting signal, the protein was found distributed non-uniformly throughout the parasite cytoplasm in all stages analyzed and co-localized with ER tracker Red, suggesting that SHLP1-GFP is located in the endoplasmic reticulum (ER).
Additional information
Other mutants
RMgm-840: A mutant lacking expression of SHLP1. |
*RMgm-841
