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Details of the target gene |
Gene Model of Rodent Parasite |
PBANKA_1113000
|
Gene Model P. falciparum ortholog |
PF3D7_0513300
|
Gene product | purine nucleoside phosphorylase |
Gene product: Alternative name | PNP |
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Details of the genetic modification |
Inducable system used | No |
Additional remarks inducable system |
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Type of plasmid/construct used | (Linear) plasmid double cross-over |
PlasmoGEM (Sanger) construct/vector used | No |
Modified PlasmoGEM construct/vector used | No
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Plasmid/construct map |
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Plasmid/construct sequence |
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Restriction sites to linearize plasmid |
KpnI, NotI
|
Partial or complete disruption of the gene | Complete |
Additional remarks partial/complete disruption |
|
Selectable marker used to select the mutant parasite | hdhfr |
Promoter of the selectable marker | eef1a |
Selection (positive) procedure | pyrimethamine |
Selection (negative) procedure | No |
Additional remarks genetic modification | Δpbpnp parasites were generated by double-crossover homologous recombination. In Δpbpnp parasites, a selection cassette containing gfp and an hDHFR–ts fusion gene was integrated into the target gene (pbpnp) |
Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences
Primer information: Primers used for amplification of the target sequences
Sequence Primer 1 | AAAGGTACCTAAAATCCACGTAACCGCCA |
Additional information primer 1 | PNP–5’F |
Sequence Primer 2 | AAACTCGAGTCCAGGATCGCCAACAACTA |
Additional information primer 2 | PNP–5’R |
Sequence Primer 3 | AAAGGATCCATGAAGGGGATTTTGACAATC |
Additional information primer 3 | PNP–3’F |
Sequence Primer 4 | AAAGCGGCCGCGGATTTCATAAATGAGCTA |
Additional information primer 4 | PNP–3’R |
Sequence Primer 5 | |
Additional information primer 5 | |
Sequence Primer 6 | |
Additional information primer 6 | |
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