Mutant/mutation
In the mutant the endogeneous cdpk1 gene is C-terminally tagged with gfp

Protein (function)
In malaria parasites, intracellular Ca²⁺ signals are translated into stage-specific cellular responses by members of a family of Ca2+-dependent protein kinases (CDPKs), which combine an N-terminal kinase domain with a C-terminal, calmodulin-like regulatory domain in the same polypeptide.

Phenotype
Phenotype analyses indicate that CDPK1 is expressed in many life cycle stages. The
C-terminal GFP tag caused no obvious growth defect in blood stages and did not interfere with parasite transmission through Anopheles stephensi mosquitoes.

Additional information
In schizonts, gametocytes, ookinetes, and young oocysts, much of the fluorescence signal emanated from the cell periphery suggesting dual N-terminal acylation may target the kinase to the plasmalemma, as has been demonstrated for CDPK1 in P. falciparum schizonts

Other mutants
RMgm-518: unsuccessful attempts to disrupt cdpk1
RMgm-773: A promoter exchange mutant that expresses CDPK1 under control of the the asexual blood stage specific clag promoter
RMgm-966: A mutant lacking expression of CDPK! (successful disruption of cdpk1!)
RMgm-967: A mutant containing a FRTed ORF of the cdpk1 locus. Analyses of this mutant indicates the lack of an essential role of CDPK1 for liver stage development