RMgmDB - Rodent Malaria genetically modified Parasites

Summary

RMgm-751
Malaria parasiteP. berghei
Genotype
Transgene
 Transgene not Plasmodium: GFP-Luciferase
Promoter: Gene model: PBANKA_1128100; Gene model (P.falciparum): PF3D7_0629300; Gene product: phosphatidylcholine-sterol acyltransferase, putative | phospholipase, putative (PL, phospholipase; UIS10)
3'UTR: Gene model: PBANKA_0719300; Gene product: bifunctional dihydrofolate reductase-thymidylate synthase, putative (dhfr/ts)
Replacement locus: Gene model: PBANKA_0306000; Gene product: 6-cysteine protein (230p)
Phenotype Oocyst;
Last modified: 12 July 2012, 09:30
  *RMgm-751
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Introduction of a transgene
Reference (PubMed-PMID number) Reference 1 (PMID number) : 22574152
MR4 number
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Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone P. berghei ANKA cl15cy1
Other information parent lineA reference wild type clone from the ANKA strain of P. berghei (PubMed: PMID: 17406255).
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The mutant parasite was generated by
Name PI/ResearcherC. Ramakrishnan; E.A. Levashina
Name Group/DepartmentInstitut de Biologie Moléculaire et Cellulaire
Name InstituteUniversité de Strasbourg
CityStrasbourg
CountryFrance
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Name of the mutant parasite
RMgm numberRMgm-751
Principal nameuis10::GFP-LUC
Alternative name787; 788
Standardized name
Is the mutant parasite cloned after genetic modificationNo
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Phenotype
Asexual blood stageNot tested
Gametocyte/GameteNot tested
Fertilization and ookineteNot tested
OocystGFP expression was detected in oocysts
SporozoiteNot tested
Liver stageNot tested
Additional remarks phenotype

Mutant/mutation
The mutant expresses the fusion protein GFP-Luciferase under the control of the uis10 promoter. The gfp-luciferase transgene has been integrated into the silent 230p locus.

Phenotype
The phenotype (GFP or luciferase expression) of this mutant has not been analysed in detail and the mutant has not been cloned.
GFP expression was detected in oocysts.

  Transgene: Mutant parasite expressing a transgene
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Type and details of transgene
Is the transgene Plasmodium derived Transgene: not Plasmodium
Transgene nameGFP-Luciferase
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Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/constructPlasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
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Plasmid/construct sequence
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TCGAGAAGAGAAGGAAGACTGACGACGCAGCCACTGCGGAACCGAGCAACGCAATGAGCT
CCTTGACGTCCACGAGGGAGACAACTCCCGTGCACGGGTTGCAGGCTCCTTCTTCGGCCG
CAGCCATTGCCCCGGTGTTGGCGTGGATGGACGAAGAAGACCGGAAAAAACGCGAGCAAA
AGGAACTGATTCGGGCCGTTCCGCATGTTCACTTTAGAGGCCATGAAGAGTTCCAGTACC
TTGATCTCATTGCCGACATTATTAACAATGGAAGGACAATGGATGACCGAACGGGCGTTG
GTGTCATCTCCAAATTCGGCTGCACTATGCGCTACTCGCTGGATCAGGCCTTTCCACTTC
TCACCACAAAGCGTGTGTTCTGGAAAGGGGTCCTCGAAGAGTTGCTGTGGTTCATTCGCG
GCGACACGAACGCAAACCATCTTTCTGAGAAGGGCGTGAAGATCTGGGACAAGAATGTGA
CACGCGAGTTCCTCGATTCGCGCAATCTCCCCCACCGAGAGGTCGGAGACATCGGCCCGG
GCTACGGCTTCCAGTGGAGACACTTCGGCGCGGCATACAAAGACATGCACACAGACTACA
CAGGGCAGGGCGTCGACCAGCTGAAGAATGTGATCCAGATGCTGAGAACGAATCCAACAG
ATCGTCGCATGCTCATGACTGCCTGGAATCCTGCAGCGCTGGACGAAATGGCGCTGCCGC
CTTGTCACTTGTTGTGCCAGTTCTACGTGAACGACCAGAAGGAGCTGTCGTGCATCATGT
ATCAGCGGTCGTGCGATGTCGGCCTCGGCGTCCCCTTCAACATCGCTTCCTATTCGCTTT
TGACGCTCATGGTTGCACACGTCTGCAACCTAAAACCTAAGGAGTTCATTCACTTCATGG
GGAACACGCATGTCTACACGAACCATGTCGAGGCTTTAAAAGAGCAGCTGCGGAGAGAAC
CGAGACCGTTCCCCATTGTGAACATCCTCAACAAGGAACGCATCAAGGAAATCGACGATT
TCACCGCCGAGGATTTTGAGGTCGTGGGCTACGTCCCGCACGGACGAATCCAGATGGAGA
TGGCTGTCTAGCGGAAATACAGAAGCTAGCTTTGATCCCGTTTTTCTTACTTATATATTT
ATACCAATTGATTGTATTTATAACTGTAAAAATGTGTATGTTGTGTGCATATTTTTTTTT
GTGCATGCACATGCATGTAAATAGCTAAAATTATGAACATTTTATTTTTTGTTCAGAAAA
AAAAAACTTTACACACATAAAATGGCTAGTATGAATAGCCATATTTTATATAAATTAAAT
CCTATGAATTTATGACCATATTAAAAATTTAGATATTTATGGAACATAATATGTTTGAAA
CAATAAGACAAAATTATTATTATTATTATTATTTTTACTGTTATAATTATGTTGTCTCTT
CAATGATTCATAAATAGTTGGACTTGATTTTTAAAATGTTTATAATATGATTAGCATAGT
TAAATAAAAAAAGTTGAAAAATTAAAAAAAAACATATAAACACAAATGATGTTTTTTCCT
TCAATTTCGATATCGCGGCCGCGAGTATAGGATAGATAATTTTTTTTGTGGATTTCCAGA
TGTCTACATATTGTATTAATATTTATTTGTGCCTTTTTTATTATTTTATGTATTTTTTTT
CGTATTTTCTTATTAATTTGTTTCCTCCTTACTAACTAATTCATAAACCTTTGAATACTT
TCTTTATCATTTTGTGTTAAAAAATTATAATTACAGTGCAAATATAATCTATATACTTCA
GTTAGTATATTAACAAAATTTAATATTTTGTATGTTTTAAGGTAATTTCATTTACTACAT
TTAAATCACATATATTACAATAATATAAATATTTCAATATATATATATTTGAATGGAACG
ATAGATACAGATTGCATATAGCTATTGAACGTGTGAAATTGCAGAAAAAATTGTTGGGTA
AATTGTAGATTAATAAAAATGAAATAATATGCATGTGCGTAAAACCTCGATAATATTTGG
TATATATGATTTTGGGAATGATATACAAATAAAGTTGTTTTATATAAACACCATACATTT
GTGTTTCTTATATTTTTCCAATTTCACATAAAATTATATAAATAGGTATACATAAATATA
TTATTGCCATGAGAAATTATTAAATATTAATAAGATTTTATGCTGCCACTCAATGTTTTT
ATTTGCTTATAAAATTATGGGTGCCAATATATTCGAAGTATTTACATGATTTATTAATAA
AATACATTTGTAAGCAAGTGTTTCAAAATCTAAAAGACGGAAAATTTCATTGTATTACAT
CAATGAAAAAAAATGTATATTATTTTATATTATTTTATATTATTCCATATTTATATTCCT
ATGAAAATTTTAATTATTACTTATAATTTCTCATTCACAAAAAGTTTTCATATAATCATA
GTTGTAAGGCTCCTATTAAATATTTTAATTATTTTAGAACAACACATATAATTACCATAT
TTTATCTCGAAATATATTTGTACAGTTTCCTCTATAATTTCAGCACAATAAAAAGTATTC
CATGCATTGTGTGCATATCTGACAACACAACCAATACTAACTACATGCGCATAATTATAT
AGTGTTTTCATTTAAAATGTGTGATTACGTTTTTATTAATATATTACATATATATTTTTT
TCATTCGAATTAAGCATAAATTATATACACGAGAAATTATAATTTATATGAATTTCTTTA
TATATTATTTCTATAAATACATAAAACCACATGCATATGCTATATTTTGTTCAACTCAAA
ATATACTAAATTAATGTAGCACCACCAAAAAAAAATAAAGCACATAAATAAGGCATGCAA
AAAAACACACTTAGGCATTGAAATGTGTTTTAATGTTTTTTCATTCAACGTAATAATATG
TTAAATATATTATTATAAAGGAAATGTTTTTATATTATATGCATATTAAAATCGATGTGA
ATTTTTTATACATTTTTTAAATTGGATTTAGTGAAAAAAAATAAATAATATTAATAAATT
CTTAAAATTTATATTATTAAATATTTGGTATATAATTCAATGTTAAATTTGTTATATGAT
AATTTTTGTCTCTAATTGTTTTTTTTTATTTTTATATATACTTTTTGATGTTTTTTATGT
CAATATTATAAAATAATTAAAATCACTATATAATAATATACTTAATTTGAGAAAATGAAA
AAGAAATTCACATAACCACCAATTTAAAGATAGTGTGTATATATATATTTTGTATAATTA
AACATTTATTGTAAAATAAATATACTATATATATAATTTGTATATATTTTTCATTAAAAA
AATTATTGGCGTAAATGTGAAAGACCATGGGGATCCATGAGTAAAGGAGAAGAACTTTTC
ACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCT
GTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGC
ACTACTGGAAAACTACCTGTTCCTTGGCCAACACTTGTCACTACTTTCGGTTATGGTGTT
CAATGCTTTGCGAGATACCCAGATCACATGAAACAGCATGACTTTTTCAAGAGTGCCATG
CCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACA
CGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATT
GATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACAC
AATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGA
CACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATT
GGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCG
AAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGG
ATTACACATGGCATGGATGAACTATACAAAGGGATCCTGGCTAGCCAGTCGACCTGCAGG
CATGCAAGCTTGCGGCCGATCCAAATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCG
CCATTCTATCCGCTGGAAGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGA
TACGCCCTGGTTCCTGGAACAATTGCTTTTACAGATGCACATATCGAGGTGAACATCACG
TACGCGGAATACTTCGAAATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTG
AATACAAATCACAGAATCGTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTG
TTGGGCGCGTTATTTATCGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGT
GAATTGCTCAACAGTATGAACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGG
TTGCAAAAAATTTTGAACGTGCAAAAAAAATTACCAATAATCCAGAAAATTATTATCATG
GATTCTAAAACGGATTACCAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTA
CCTCCCGGTTTTAATGAATACGATTTTGTACCAGAGTCCTTTGATCGTGACAAAACAATT
GCACTGATAATGAATTCCTCTGGATCTACTGGGTTACCTAAGGGTGTGGCCCTTCCGCAT
AGAACTGCCTGCGTCAGATTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATT
CCGGATACTGCGATTTTAAGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACA
CTCGGATATTTGATATGTGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTG
TTTTTACGATCCCTTCAGGATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTT
TCATTCTTCGCCAAAAGCACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATT
GCTTCTGGGGGCGCACCTCTTTCGAAAGAAGTCGGGGAAGCGGTTGCAAAACGCTTCCAT
CTTCCAGGGATACGACAAGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACA
CCCGAGGGGGATGATAAACCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAG
GTTGTGGATCTGGATACCGGGAAAACGCTGGGCGTTAATCAGAGAGGCGAATTATGTGTC
AGAGGACCTATGATTATGTCCGGTTATGTAAACAATCCGGAAGCGACCAACGCCTTGATT
GACAAGGATGGATGGCTACATTCTGGAGACATAGCTTACTGGGACGAAGACGAACACTTC
TTCATAGTTGACCGCTTGAAGTCTTTAATTAAATACAAAGGATATCAGGTGGCCCCCGCT
GAATTGGAATCGATATTGTTACAACACCCCAACATCTTCGACGCGGGCGTGGCAGGTCTT
CCCGACGATGACGCCGGTGAACTTCCCGCCGCCGTTGTTGTTTTGGAGCACGGAAAGACG
ATGACGGAAAAAGAGATCGTGGATTACGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTG
CGCGGAGGAGTTGTGTTTGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACTCGACGCA
AGAAAAATCAGAGAGATCCTCATAAAGGCCAAGAAGGGCGGAAAGATCGCCGTGTAATTC
TAGAAGATCCCGTTTTTCTTACTTATATATTTATACCAATTGATTGTATTTATAACTGTA
AAAATGTGTATGTTGTGTGCATATTTTTTTTTGTGCATGCACATGCATGTAAATAGCTAA
AATTATGAACATTTTATTTTTTGTTCAGAAAAAAAAAACTTTACACACATAAAATGGCTA
GTATGAATAGCCATATTTTATATAAATTAAATCCTATGAATTTATGACCATATTAAAAAT
TTAGATATTTATGGAACATAATATGTTTGAAACAATAAGACAAAATTATTATTATTATTA
TTATTTTTACTGTTATAATTATGTTGTCTCTTCAATGATTCATAAATAGTTGGACTTGAT
TTTTAAAATGTTTATAATATGATTAGCATAGTTAAATAAAAAAAGTTGAAAAATTAAAAA
AAAACATATAAACACAAATGATGTTTTTTCCTTCAATTTCGGGTACCGAGCTCGAATTCT
CTTGAGCCCGTTAATGAAATAGATACAATTCATTCATGTTATATACATCTAGAACATAAT
CTGAATATGGTTCAAGTTAAATGTCCAAAAATTATAAAAAGTGATGATATTTTTGATGGT
AATACCATAATAGACACCAAGGTAACATCACGAAGTAGTCAACAAAATAATTTTTATTTA
GAAAATACAGATGTTGAACCAGAAGAAATAGAGAAATATAAAAATATAGAATACATACCA
GAAAACGATGAAGTAATGCATCTAGACAAAAAAGAAAAGCTAGATGATATATTACCAGGT
GTTATCATATTAGATAAAAATAAAATGTTCAAAGAAAAAGGACATTTCACTTTTGTTACT
CCATTAATTGTAGAAAAGGTATTAATATTAAAAATATATTGTGATAATACTAAAACAATA
ATTAATAATATGAAAGGGAAAAAAGGTATTACAGTAATAAGGATTTCTCAAAATACAACA
AAAAATAAATTTTATGGATGTGACTTTTCAGGTAATTCTAAAAAAACATTTTACTATTCC
AATGTTTATGATTTAGAAAAAAAAAATGAGTTTTGTGAAATAGAATTAAAAGAAAATATA
GTAGTTAGCTTAAATTGTCCAACTGGTAAAATTAATCCAAAAAATTGTTTTAGAAATGTA
TATATAAAAAGTAATATGAATGAACAAACAACCGAAAATATAGAAAATATATTTAACGAA
ATAAAAGTTATAGATGCAGATTATTTTATAAATAATTCATCAACCTTTTTGATGATTTCC
AAAATTACAAAAAAAGAGTTTGATTTTTATTGTACATGTGAAGATTATAAAACCAAAAAT
ATAGGAACAATATATATTAAAAATTATGAATATCTAGATTCAAAACCTAAATATAAAAAT
AAACAAATTTCCTATATAGATGTAGTTCCATACCCGCGGGGAAAGGGCGAATTCACTGGC
CGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGC
AGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTC
CCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCA
TCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGC
ATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCT
GCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAG
GTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTT
ATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAA
TGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCAT
GAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCA
ACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCA
CCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTA
CATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTT
TCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGC
CGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTC
ACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGC
CATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAA
GGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGA
ACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAAT
GGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACA
ATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCC
GGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCAT
TGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAG
TCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAA
GCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCA
TTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCC
TTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTC
TTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACC
AGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTT
CAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTT
CAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGC
TGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAA
GGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGAC
CTACACCGAACTGAGATACCTACAGCGTGAGCATTGAGAAAGCGCCACGCTTCCCGAAGG
GAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGA
GCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACT
TGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAA
CGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGC
GTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCG
CCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCCAAT
ACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTT
TCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTA
GGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGG
ATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTTCCGCGGGTATA
TGGTAAAGAACCTACTAACACAATAAAATATTTAAATAATGTATTTCCTATAAATAAATT
TACAGATTTATTTTTTAATACAAAAGATATAGATATACCAGAAATAAATGATCAGTTTAA
AGGTTTTAAATTCTTTATGACATCATTTATAAATCATGGATCATATCCACTAACAATAGA
ATGTGGTGTAACAAATGGTGGAACTAGTTATAAAAGAGCAATTATTTTATTGCATGTTCG
AACTGATTTAAAAGATAGACCAGTTTCATTTTGTGATTTTCGAAAAGGAGAATTATATAA
TTATTTGAATGCTTATACTGAAGGGGATGTATGCATAATAATTTCCAAATCAAATACAAG
TTTTGGTTTTAGATGCCCAGTAAATACAAAAAAAATGCCAAAAAATTGTTTTACGCAAGT
ATATGAAAAAGGGTATCTAAATGACGCCAATAAAATTAATACTAAAAATGTTATTAACTA
TTCATTTGAAAATCCAGAATATGCGCTAGCTGGTTYTAATTATACATTAACAAAATCGTA
TCAATTTGAATGTCATTGTGTAGATAAAGAAACAGAACAAATTGTAAAAACGGTTTTAGT
CAAATATGTAAATGAAGATGAAATATATGATTATAATGATTTTCCAATGGTGAATCACAA
ACCTATTATTGCACATCCAAATAAAACACATCAAGCTTGCATGCCTGCAGGTCAACAATA
AATAATAAATAAATATTGTGGAAATAAAATAACATATAATTATTTTTAATACATTGATTT
CCCTTTTATTTTTTTAAATTTCATTGATATAAAAATATATAATAATAACATATATGATTT
CAAATTAATCTTTTCAAAAATGGTGTTTATTTTTGTATGTTTGTGTATGAATTAATCACA
TAACACATCTATTAAATTGAGTTGGTAATATAGACACAAATAAATATATATATTTTTATA
GCTTAAAAGTGTGTTATGAATATTTTAAGCATATTTTCTTTTTCTTTGGATTGTGTAAAA
TGAACTCATATAATGCGTTTTTTTGTTTTTGTTATTTTGTCATTTTGTTATTTTGCTATT
TTATGGATTAATTTTTGTTTATAAAATGGGAAATAATTTTAACATATTTAAATAAATGGA
GAAAAAATATAAAATAATTATAAAAAAAAGTTAATACACATTTTTTCCTGTTATAGACCT
TATATTTATTTATCCATATATATATATATATATATATATATATATATATACATACCAAGT
GAATTAAGAGGAAAGCTAATTTATTATTCAGAATAATATATGAACTATATATAATTTTTA
TTATTTTGGTGTATATTAATCTGTCTATATGCATACATGCAATAATTTATCGACTTATAT
ATCAAATAACATAAAATAGAAGTGTTTTAAATTATGGATATATGCTCAATATTCATTTTT
TTTAATAAGTTAGCTATATTTAAATTATACATTTTATATATGGTCTCTTTTTTTTTTAAA
TATTATTTAAGTGATCATGAAAATATAAATAATTTTTTTTTATTTAATATCCTTTTGCTT
GCATGTGGTAAATGGAAATTTGGATGTGTTTTGAARGTTCGGATATAGTTGTATGGACAT
ATAATATATTTTGTGAAAAATTGGTTTTATGTTTATACTTATGCCAATACTTTTTGAGTA
AAACAAAGCAAGTGCTTATAAATAATTAAAGCCAATTTTATAATATATATTTTTTTATTT
AATTTGAATTTAGTAGTATAATTTTTTATGGTAAGTGCTCAAAGAGAGTTGCTTATAAAG
TATGGTTTGTTTCTTTTTCGCCATTTTGAATTACACATTAAAAATATATAGATACATATA
TTATAATATGAAATCATTAATAATTTAGGGAAATTCTACAAATTTAAAAACGAATAAAAT
AATTGTTTTTCATCATGCCATAACACAATATTGATATATACATGTACAAACATTTTTTTT
ATTTGGAAAATATAAATTATATAAAAAAAAATGTATAGTATACAAAATGAGCATATTCAC
ACGGGGTGGACGTTCATTTTTTCATTTTTCCCCTGTTTTTTATGAGTATATGATAAAATT
TTATGAACATTTACACAAAATGAAAATGGATATATAGGAAAAATGGAGCGGTATTTCATT
TATCTTTGATTGTCATTTGGATATTATATTACCYTGGGTAGGCAATTAAAAATGTTAAAT
AACAATTTAAGGAAATTATATTTTATATATTAAAATTAACACTGTATTATATGATTCGCT
TATAAAAGCCACTCTTTCCCCATGCAAAGCTGTTTAATATCAATTTTAACAAATTACACA
CATGTTAATATATTTATATATATAATTTATATATTTATAATTTATATATTTATATTTTTA
TTATTTATATATTTATTATTTATTGTGTGTGTCAATTCGGGTAGGATATACCTCTTTTTT
ATTGTTTAAAGCGATTTGTATTCTAAAATATAAAGRATTTGAAAAAGAGAAAGATAGAAT
ATGATCCCATCATATATAGCCCTATAATTTTTATTTAGCAGCGAATTAATTTTTCTATTA
AGTTTATGTGTAATTAAAATAACGGAATATATATAATACAATAAAAAAGTGCATAAATTA
AAATTTTTTCAATTAAATTTTTTTTTTTAAGGGGTTATATAATATTAAATATATAAAATA
CGATTATATATTTTTGCTACAATTTTTTATATTAAGATATAAATAGTAAATAAATGGTAT
TATATGGCATGTAATATATAAATTTTTTCCAATTTTTATTTTATATACACTTTTCCTTTT
TTTGTCATAAAACTTAAACAATTTACACATTCATTTTAAAAATTGACTATTTGTTTCAAC
ATTTTTTGAGTTTCCGTTTTATAATAGTATTTTCATTTGTATATTGCTTATATATATAAA
TACACACCTAAATGTTACAAAGGATCAATGCATAAACCGGTGTGTCTGGTCGTCGCGATG
ACCCCCAAGAGGGGCATCGGCATCAACAACGGCCTCCCGTGGCCCCACTTGACCACAGAT
TTCAAACACTTTTCTCGTGTGACAAAAACGACGCCCGAAGAAGCCAGTCGCCTGAACGGG
TGGCTTCCCAGGAAATTTGCAAAGACGGGCGACTCTGGACTTCCCTCTCCATCAGTCGGC
AAGAGATTCAACGCCGTTGTCATGGGACGGAAAACCTGGGAAAGCATGCCTCGAAAGTTT
AGACCCCTCGTGGACAGATTGAACATCGTCGTTTCCTCTTCCCTCAAAGAAGAAGACATT
GCGGCGGAGAAGCCTCAAGCTGAAGGCCAGCAGCGCGTCCGAGTCTGTGCTTCACTCCCA
GCAGCTCTCAGCCTTCTGGAGGAAGAGTACAAGGATTCTGTCGACCAGATTTTTGTCGTG
GGAGGAGCGGGACTGTACGAGGCAGCGCTGTCTCTGGGCGTTGCCTCTCACCTGTACATC
ACGCGTGTAGCCCGCGAGTTTCCGTGCGACGTTTTCTTCCCTGCGTTCCCCGGAGATGAC
ATTCTTTCAAACAAATCAACTGCTGCGCAGGCTGCAGCTCCTGCCGAGTCTGTGTTCGTT
CCCTTTTGTCCGGAGCTCGGAAGAGAGAAGGACAATGAAGCGACGTATCGACCCATCTTC
ATTTCCAAGACCTTCTCAGACAACGGGGTTCCCTACGACTTTGTGGTTC
Restriction sites to linearize plasmid KspI (SacII)
Selectable marker used to select the mutant parasitetgdhfr
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
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Other details transgene
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Promoter
Gene Model of Parasite PBANKA_1128100
Gene Model P. falciparum ortholog PF3D7_0629300
Gene productphosphatidylcholine-sterol acyltransferase, putative | phospholipase, putative
Gene product: Alternative namePL, phospholipase; UIS10
Primer information details of the primers used for amplification of the promoter sequence  Click to view information
Primer information details of the primers used for amplification of the promoter sequence  Click to hide information
Sequence Primer 1CGGATATCGCGGCCGCGAGTATAGGATAGATAATTTTTTTTGTGG
Additional information primer 12603 (EcoRV/NotI); UIS10 promoter sense
Sequence Primer 2CGGGATCCCCATGGTCTTTCACATTTACGCCAATAATTTTTTTAATG
Additional information primer 22604 (NcoI/BamHI); UIS10 promoter antisense
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3'-UTR
Gene Model of Parasite PBANKA_0719300
Gene productbifunctional dihydrofolate reductase-thymidylate synthase, putative
Gene product: Alternative namedhfr/ts
Primer information details of the primers used for amplification the 3'-UTR sequences  Click to view information
Primer information details of the primers used for amplification the 3'-UTR sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Insertion/Replacement locus
Replacement / InsertionReplacement locus
Gene Model of Parasite PBANKA_0306000
Gene product6-cysteine protein
Gene product: Alternative name230p
Primer information details of the primers used for amplification of the target sequences  Click to view information
Primer information details of the primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
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Conceptual design of the database: Dr. C.J. Janse (Leiden Malaria Research Group, Leiden, The Netherlands).
Technical design and realization: S. Mededovic and A. van Wigcheren