Summary

RMgm-712
Malaria parasiteP. berghei
Genotype
TaggedGene model (rodent): PBANKA_1400600; Gene model (P.falciparum): Not available; Gene product: cytoadherence linked asexual protein, putative (CLAG3)
Name tag: mCherry
Phenotype Asexual bloodstage;
Last modified: 21 March 2013, 17:57
  *RMgm-712
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene tagging
Reference (PubMed-PMID number) Reference 1 (PMID number) : 23197789
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone P. berghei ANKA cl15cy1
Other information parent lineA reference wild type clone from the ANKA strain of P. berghei (PubMed: PMID: 17406255).
The mutant parasite was generated by
Name PI/ResearcherE.M. Pasini, C.J. Janse, B.M.D. Franke-Fayard
Name Group/DepartmentDepartment of Parasitology
Name InstituteBiomedical Primate Research Centre
CityRijswijk
CountryThe Netherlands
Name of the mutant parasite
RMgm numberRMgm-712
Principal name1915
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationNo
Phenotype
Asexual blood stageThe tagged-protein shows a rhoptry-like location in merozoites
Gametocyte/GameteNot tested
Fertilization and ookineteNot tested
OocystNot tested
SporozoiteNot tested
Liver stageNot tested
Additional remarks phenotype

Mutant/mutation

Protein (function)

Phenotype

Additional information
The mutant parasite expresses an mCherry-tagged protein. The protein has been selected for tagging in a screen for putative exported proteins of P. berghei.

The genotype of the parasites has not been analyzed in detail.
Southern analysis of PFG-separated chromosomes (to show integration into the chromosome on which the target gene is located) has been performed (see below). This analysis provided evidence for tagging of the correct gene. The construct used aims at integration of the tagging construct by single-cross-over integration resulting in the presence of a tagged copy of the endogenous gene.

Phenotype analyses of the blood stages by fluorescence microscopy show that the tagged-protein has a rhoptry-like location in merozoites.

P. falciparum orthologues

PFB0935w: PF3D7_0220800: cytoadherence linked asexual protein 2; CLAG2; RhopH1(2)
PFC0110w: PF3D7_0302200: cytoadherence linked asexual protein 3.2; CLAG3.2; RhopH1(3.2)
PFC0120w: PF3D7_0302500: cytoadherence linked asexual protein 3.1; CLAG3.1; RhopH1(3.1)



Other mutants
RMgm-724: unsuccessful attempt to disrupt PBANKA_140060


  Tagged: Mutant parasite with a tagged gene
Details of the target gene
Gene Model of Rodent Parasite PBANKA_1400600
Gene Model P. falciparum ortholog Not available
Gene productcytoadherence linked asexual protein, putative
Gene product: Alternative nameCLAG3
Details of the genetic modification
Name of the tagmCherry
Details of taggingC-terminal
Additional remarks: tagging
Commercial source of tag-antibodiesClontech
Type of plasmid/constructPlasmid single cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
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Plasmid/construct sequence
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TCTGCATACAACGTTCTCTGTAAAAATTAACAAAAATCTTGATAAGTTGGACAAAGATTA
TGCAAAGGCGAAATTAATTGAGCTTTCTGCGGATGAAAATGCTTATTTCACGTATGCGTA
TGCATATTATGGTAGCATAATTGATAGCATAACGAACAGCTTGATGCCCTTATATGCGAA
GAAACCCATTACACAACTGCGTTATGGAAAAACTTTTATTTTTGCGAATTATTTTGGATT
TGCAGCACAGATATACGGAATTCTTAATCTTAATAATATGAAAATGTTGTTTGAGCATCA
AGCAATTGCATCGGCGAACAGCTATTCCATTGAGAAGATGTCACAATACTTGGCAAAAAA
ATATGTATCGATTGTTGTATACTTTGGTGTGCTCAGGGGTTACGATGTGAGTACTTATCC
ATCAGAATTGACATTTTTGAGTAATGTATTATTTAAGAGTACTTTTTTCCATGTTTCAAT
GTACTTCACTATGCATATAGGTACTCTTCTATGGTTAGAAAGTGGAAGGGTGCTACCAAG
GCCAATTTACAACGAACTACAGGATCAGACGAGTGATATGTTTATACAAGAACCACCAGA
GAAAGGGCCATTGCATTCATTCGGCAAAGATTTTGTATTTGGCATGGTTACCGCGTTTAC
TTTTCTGTTTTCAGTTTATACTATTTTCCGTTGGTATGCATTTTACACGAATGTGGTATA
CTTCTTTATTATGCCAGTAAGGCTTTTCAGTTCTTATCATAGACCTTTCCAAAATAAGTT
TTCCACAGTCACAATAGATATTATGAAAAGTATAACGGCAGATCCAATTTTAAAGTTGAT
GAACAAAATATTGCGAAGCATGAAAAGTAGAAACGAGTACAACGAGATTATGAATGCTAG
ATTAAATGACACAAATTTTAAAAGATTTATAGAGGGAAAGCGAAGTAGTGCAATCATGCC
GAGTATGACACAAATGATGGATTTAGATAGAATGACCTATACATTGTATAAAGATGACAA
CGTGTTCTTTGATGGCGTGAGTAAGGAGGAAGAGTTCCTGAACCAAAGGAGCATAATAGG
ATCCGGTGGAGGTGGAAGATCCATGGTGAGCAAGGGCGAGGAGGATAACATGGCCATCAT
CAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGAACGGCCACGAGTTCGA
GATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGACCGCCAAGCTGAAGGT
GACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCCCTCAGTTCATGTACGG
CTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACTTGAAGCTGTCCTTCCC
CGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCGGCGTGGTGACCGTGAC
CCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGAAGCTGCGCGGCACCAA
CTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCTGGGAGGCCTCCTCCGA
GCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGCAGAGGCTGAAGCTGAA
GGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGGCCAAGAAGCCCGTGCA
GCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCTCCCACAACGAGGACTA
CACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCACCGGCGGCATGGACGA
GCTGTACAAGTAATCTAGAATTATTAATATATATGAATATATATACATCGTTGTATGCGT
AATATACGTGTAACTGTTTTTTTTAATTTTTATATTAACTTTTCCTTATACATTTTAATG
CCATTAACTAATAATATTTTACTTGAAAAAATTCTAAATTTTTATAACTCTAATATAAAA
TCTGTGAATAATAAAAAATTAGCATAAAGTACGAATTAAACATCGAATATTTGTTTTAAA
GGGGCACACATTATGTGCATATAATTTTATTCTTTGAATTTAATTTTTTGCTTATAATTA
GATTAAATTAATATAATTTTTTTATTGAAAATAAAAACTTATTTTAAAAAATTAACAAAT
GGAAGTTAATGAAAAATACGAAAAAAGTAAAGGGTTAATTCTTATATGGTCGGTACCGAG
CTCGAATTCGATATCGAAATTGAAGGAAAAAACATCATTTGTGTTTATATGTTTTTTTTT
AATTTTTCAACTTTTTTTATTTAACTATGCTAATCATATTATAAACATTTTAAAAATCAA
GTCCAACTATTTATGAATCATTGAAGAGACAACATAATTATAACAGTAAAAATAATAATA
ATAATAATAATTTTGTCTTATTGTTTCAAACATATTATGTTCCATAAATATCTAAATTTT
TAATATGGTCATAAATTCATAGGATTTAATTTATATAAAATATGGCTATTCATACTAGCC
ATTTTATGTGTGTAAAGTTTTTTTTTTCTGAACAAAAAATAAAATGTTCATAATTTTAGC
TATTTACATGCATGTGCATGCACAAAAAAAAATATGCACACAACATACACATTTTTACAG
TTATAAATACAATCAATTGGTATAAATATATAAGTAAGAAAAACGGGATCAAAGCTAGCT
TCTGTATTTCCGCTAGACAGCCATCTCCATCTGGATTCGTCCGTGCGGGACGTAGCCCAC
GACCTCAAAATCCTCGGCGGTGAAATCGTCGATTTCCTTGATGCGTTCCTTGTTGAGGAT
GTTCACAATGGGGAACGGTCTCGGTTCTCTCCGCAGCTGCTCTTTTAAAGCCTCGACATG
GTTCGTGTAGACATGCGTGTTCCCCATGAAGTGAATGAACTCCTTAGGTTTTAGGTTGCA
GACGTGTGCAACCATGAGCGTCAAAAGCGAATAGGAAGCGATGTTGAAGGGGACGCCGAG
GCCGACATCGCACGACCGCTGATACATGATGCACGACAGCTCCTTCTGGTCGTTCACGTA
GAACTGGCACAACAAGTGACAAGGCGGCAGCGCCATTTCGTCCAGCGCTGCAGGATTCCA
GGCAGTCATGAGCATGCGACGATCTGTTGGATTCGTTCTCAGCATCTGGATCACATTCTT
CAGCTGGTCGACGCCCTGCCCTGTGTAGTCTGTGTGCATGTCTTTGTATGCCGCGCCGAA
GTGTCTCCACTGGAAGCCGTAGCCCGGGCCGATGTCTCCGACCTCTCGGTGGGGGAGATT
GCGCGAATCGAGGAACTCGCGTGTCACATTCTTGTCCCAGATCTTCACGCCCTTCTCAGA
AAGATGGTTTGCGTTCGTGTCGCCGCGAATGAACCACAGCAACTCTTCGAGGACCCCTTT
CCAGAACACACGCTTTGTGGTGAGAAGTGGAAAGGCCTGATCCAGCGAGTAGCGCATAGT
GCAGCCGAATTTGGAGATGACACCAACGCCCGTTCGGTCATCCATTGTCCTTCCATTGTT
AATAATGTCGGCAATGAGATCAAGGTACTGGAACTCTTCATGGCCTCTAAAGTGAACATG
CGGAACGGCCCGAATCAGTTCCTTTTGCTCGCGTTTTTTCCGGTCTTCTTCGTCCATCCA
CGCCAACACCGGGGCAATGGCTGCGGCCGAAGAAGGAGCCTGCAACCCGTGCACGGGAGT
TGTCTCCCTCGTGGACGTCAAGGAGCTCATTGCGTTGCTCGGTTCCGCAGTGGCTGCGTC
GTCAGTCTTCCTTCTCTTCTCGAGAACCACAAAGTCGTAGGGAACCCCGTTGTCTGAGAA
GGTCTTGGAAATGAAGATGGGTCGATACGTCGCTTCATTGTCCTTCTCTCTTCCGAGCTC
CGGACAAAAGGGAACGAACACAGACTCGGCAGGAGCTGCAGCCTGCGCAGCAGTTGATTT
GTTTGAAAGAATGTCATCTCCGGGGAACGCAGGGAAGAAAACGTCGCACGGAAACTCGCG
GGCTACACGCGTGATGTACAGGTGAGAGGCAACGCCCAGAGACAGCGCTGCCTCGTACAG
TCCCGCTCCTCCCACGACAAAAATCTGGTCGACAGAATCCTTGTACTCTTCCTCCAGAAG
GCTGAGAGCTGCTGGGAGTGAAGCACAGACTCGGACGCGCTGCTGGCCTTCAGCTTGAGG
CTTCTCCGCCGCAATGTCTTCTTCTTTGAGGGAAGAGGAAACGACGATGTTCAATCTGTC
CACGAGGGGTCTAAACTTTCGAGGCATGCTTTCCCAGGTTTTCCGTCCCATGACAACGGC
GTTGAATCTCTTGCCGACTGATGGAGAGGGAAGTCCAGAGTCGCCCGTCTTTGCAAATTT
CCTGGGAAGCCACCCGTTCAGGCGACTGGCTTCTTCGGGCGTCGTTTTTGTCACACGAGA
AAAGTGTTTGAAATCTGTGGTCAAGTGGGGCCACGGGAGGCCGTTGTTGATGCCGATGCC
CCTCTTGGGGGTCATCGCGACGACCAGACACACCGGTTTATGCATTGATCCTTTGTAACA
TTTAGGTGTGTATTTATATATATAAGCAATATACAAATGAAAATACTATTATAAAACGGA
AACTCAAAAAATGTTGAAACAAATAGTCAATTTTTAAAATGAATGTGTAAATTGTTTAAG
TTTTATGACAAAAAAAGGAAAAGTGTATATAAAATAAAAATTGGAAAAAATTTATATATT
ACATGCCATATAATACCATTTATTTACTATTTATATCTTAATATAAAAAATTGTAGCAAA
AATATATAATCGTATTTTATATATTTAATATTATATAACCCCTTAAAAAAAAAAATTTAA
TTGAAAAAATTTTAATTTATGCACTTTTTTATTGTATTATATATATTCCGTTATTTTAAT
TACACATAAACTTAATAGAAAAATTAATTCGCTGCTAAATAAAAATTATAGGGCTATATA
TGATGGGATCATATTCTATCTTTCTCTTTTTCAAATYCTTTATATTTTAGAATACAAATC
GCTTTAAACAATAAAAAAGAGGTATATCCTACCCGAATTGACACACACAATAAATAATAA
ATATATAAATAATAAAAATATAAATATATAAATTATAAATATATAAATTATATATATAAA
TATATTAACATGTGTGTAATTTGTTAAAATTGATATTAAACAGCTTTGCATGGGGAAAGA
GTGGCTTTTATAAGCGAATCATATAATACAGTGTTAATTTTAATATATAAAATATAATTT
CCTTAAATTGTTATTTAACATTTTTAATTGCCTACCCARGGTAATATAATATCCAAATGA
CAATCAAAGATAAATGAAATACCGCTCCATTTTTCCTATATATCCATTTTCATTTTGTGT
AAATGTTCATAAAATTTTATCATATACTCATAAAAAACAGGGGAAAAATGAAAAAATGAA
CGTCCACCCCGTGTGAATATGCTCATTTTGTATACTATACATTTTTTTTTATATAATTTA
TATTTTCCAAATAAAAAAAATGTTTGTACATGTATATATCAATATTGTGTTATGGCATGA
TGAAAAACAATTATTTTATTCGTTTTTAAATTTGTAGAATTTCCCTAAATTATTAATGAT
TTCATATTATAATATATGTATCTATATATTTTTAATGTGTAATTCAAAATGGCGAAAAAG
AAACAAACCATACTTTATAAGCAACTCTCTTTGAGCACTTACCATAAAAAATTATACTAC
TAAATTCAAATTAAATAAAAAAATATATATTATAAAATTGGCTTTAATTATTTATAAGCA
CTTGCTTTGTTTTACTCAAAAAGTATTGGCATAAGTATAAACATAAAACCAATTTTTCAC
AAAATATATTATATGTCCATACAACTATATCCGAACYTTCAAAACACATCCAAATTTCCA
TTTACCACATGCAAGCAAAAGGATATTAAATAAAAAAAAATTATTTATATTTTCATGATC
ACTTAAATAATATTTAAAAAAAAAAGAGACCATATATAAAATGTATAATTTAAATATAGC
TAACTTATTAAAAAAAATGAATATTGAGCATATATCCATAATTTAAAACACTTCTATTTT
ATGTTATTTGATATATAAGTCGATAAATTATTGCATGTATGCATATAGACAGATTAATAT
ACACCAAAATAATAAAAATTATATATAGTTCATATATTATTCTGAATAATAAATTAGCTT
TCCTCTTAATTCACTTGGTATGTATATATATATATATATATATATATATATATATATGGA
TAAATAAATATAAGGTCTATAACAGGAAAAAATGTGTATTAACTTTTTTTTATAATTATT
TTATATTTTTTCTCCATTTATTTAAATATGTTAAAATTATTTCCCATTTTATAAACAAAA
ATTAATCCATAAAATAGCAAAATAACAAAATGACAAAATAACAAAAACAAAAAAACGCAT
TATATGAGTTCATTTTACACAATCCAAAGAAAAAGAAAATATGCTTAAAATATTCATAAC
ACACTTTTAAGCTATAAAAATATATATATTTATTTGTGTCTATATTACCAACTCAATTTA
ATAGATGTGTTATGTGATTAATTCATACACAAACATACAAAAATAAACACCATTTTTGAA
AAGATTAATTTGAAATCATATATGTTATTATTATATATTTTTATATCAATGAAATTTAAA
AAAATAAAAGGGAAATCAATGTATTAAAAATAATTATATGTTATTTTATTTCCACAATAT
TTATTTATTATTTATTGTTGACCTGCAGGCATGCAAGCTTATCGCGATACCGTCGACCTC
GAGGGGGGGCCCGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTAATTGCGCGCTTGGC
GTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAA
CATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCAC
ATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCA
TTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTC
CTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTC
AAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGC
AAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAG
GCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCC
GACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGT
TCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCT
TTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGG
CTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCT
TGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGAT
TAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGG
CTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAA
AAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGT
TTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTC
TACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATT
ATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTA
AAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTAT
CTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAAC
TACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACG
CTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAG
TGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGT
AAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGT
GTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGT
TACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGT
CAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCT
TACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATT
CTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATAC
CGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAA
ACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAA
CTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCA
AAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCT
TTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGA
ATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACC
TAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCA
TTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAG
ATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCC
AACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCC
TAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGC
CCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAA
GCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACC
ACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCATTCAGGCTGCGC
AACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGGG
GGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTGT
AAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAATTGGAGCTCCAC
CGCGGTGGCGGCCGCTCTAGAACTAG
Restriction sites to linearize plasmid ClaI
Selectable marker used to select the mutant parasitetgdhfr
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1AACTAGTCTGCATACAACGTTCTCTG
Additional information primer 1L6368 (SpeI); PBANKA_140060 Forward
Sequence Primer 2AGGATCCTATTATGCTCCTTTGGTTCAGG
Additional information primer 2L6369 (BamHI); PBANKA_140060 Reverse
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6