Summary

RMgm-699
Malaria parasiteP. berghei
Genotype
TaggedGene model (rodent): PBANKA_0316700; Gene model (P.falciparum): Not available; Gene product: fam-b protein (fam-b protein)
Name tag: mCherry
Phenotype Asexual bloodstage; Liver stage;
Last modified: 16 August 2015, 16:50
  *RMgm-699
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene tagging
Reference (PubMed-PMID number) Reference 1 (PMID number) : 23197789
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone P. berghei ANKA cl15cy1
Other information parent lineA reference wild type clone from the ANKA strain of P. berghei (PubMed: PMID: 17406255).
The mutant parasite was generated by
Name PI/ResearcherE.M. Pasini, C.J. Janse, B.M.D. Franke-Fayard
Name Group/DepartmentDepartment of Parasitology
Name InstituteBiomedical Primate Research Centre
CityRijswijk
CountryThe Netherlands
Name of the mutant parasite
RMgm numberRMgm-699
Principal name1599cl4
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationYes
Phenotype
Asexual blood stageThe tagged-protein is exported into the cytoplasm of the host erythrocyte and shows a diffuse or patchy localization in the cytoplasm
Gametocyte/GameteNot tested
Fertilization and ookineteNot tested
OocystNot different from wild type
SporozoiteNot different from wild type
Liver stageThe tagged protein is expressed in late liver stages (schizonts) where it is located in the parasitophorous vacuole.
Additional remarks phenotype

Mutant/mutation

Protein (function)

Phenotype

Additional information
The mutant parasite expresses an mCherry-tagged protein. The protein has been selected for tagging in a screen for putative exported proteins of P. berghei.

The genotype of the parasites has not been analysed in detail.
Southern analysis of PFG-separated chromosomes (to show integration into the chromosome on which the target gene is located) has been performed (see below). This analysis provided evidence for tagging of the correct gene. The construct used aims at integration of the tagging construct by single-cross-over integration resulting in the presence of a tagged copy of the endogenous gene.

Phenotype analyses of the blood stages by fluorescence microscopy show that the tagged-protein is exported into the cytoplasm of the host erythrocyte and shows a diffuse or patchy localization in the cytoplasm.

The protein is not detected in oocysts and in sporozoites.

The tagged protein is expressed in late liver stages (schizonts) where it is located in the parasitophorous vacuole.




Other mutants


  Tagged: Mutant parasite with a tagged gene
Details of the target gene
Gene Model of Rodent Parasite PBANKA_0316700
Gene Model P. falciparum ortholog Not available
Gene productfam-b protein
Gene product: Alternative namefam-b protein
Details of the genetic modification
Name of the tagmCherry
Details of taggingC-terminal
Additional remarks: tagging
Commercial source of tag-antibodiesClontech
Type of plasmid/construct(Linear) plasmid single cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
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Plasmid/construct sequence
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CTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTC
ATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGA
GATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTC
CAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACC
CTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAG
CCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAA
AGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCAC
CACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCATTCAGGCTGCG
CAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCTATTACGCCAGCTGGCGAAAGG
GGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAGGGTTTTCCCAGTCACGACGTTG
TAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCACTATAGGGCGAATTGGAGCTCCA
CCGCGGCTATATGGTGAAATTCTCTATTATAACTGAATTATAATATTTATTTGACTTAAT
TATTATTTATAAAAGTCAAAATATTGTAGTGTTTAAATACAAAAAGCGTCTTAACATCTG
TTAAATGAATATAATGAAAATAAAACTTAATAGTATTAAGTGTATTTCATTTTTCGACTT
GAGATAAAAATAAATTGGTATCTCATTCAATATATTGGTTCTATATGTATTATTTTTGTG
ATTTAATATTATAATTTGATTATTTGTTTGAATTATATTTGAAAATATGTATATTTATAT
TATTTTAATAGAAACCATATATTTGATGTTGTATATTCATAGATATATTTATAAATAAAA
TTATTACATACTAATATATAAAAAAATATATTTTTACACAAAAATGGTAAATAAAATGGA
GAATATTATTAAGTAATACTTAAAATTGGAGAAGTATAACAAATAAGAAGCCATTTAAAT
ATAAATAGCAACAATGATTTGTTAATTTTTTCATTATGTCTTTATTTATTCATATAAAAA
AATATATCCAATATTTTATTATTTTAATAGATATTTATTAATGTGTTCATATGTAAACAA
TAATAGGAAAATATAAGTTTATAAAATATTAAAAACTTACCACTAAAATCACCAGCGTAT
TATAAATTATATTAAAAGCCTATTTGTATACCAATTTTCCAAAAAATATATTTTATGGAA
AATTATTATATACATATATTTTTTTATAATTAAAATATTAAAAACGGTGTATTTTTTAGA
GAAAAGTTAATATTTCTTTGAAAAAAAATATAATATTTATATCCATAAACAATTTTAATG
AAATAACGAAATTTATTATAACAATTACATTAATTAATTTTATAGAGTGTAAAAAGTTAA
TAAATGAAATAAATTCGTTTTTTTTTATAGATATATTCATTTATTCATAAATTTTAGAAC
AAAAAACTAGTAAATATTGCTATTTTGTGAATAAATAGCAAGTTACTGACATTATCTCAA
ACTACATATATTAGTTCATTTATTTAATAAATAAAGGTAAACATGAGAATCAGTATTTTA
AAATATGTTCTTTTTTCAATTGTTATTTGTTCTTTTGAATATGCCAAAAATGTAAGTTAC
ACTTTGTTTTCTTTTATTATTAATAATATTTCATTATAGTTTTCGTATCTATCGTTTTAC
ATTAACCTTATATTATTGTTTCATGTATTGGTAAAACACGTGTGTTAAGTTAAACCAACA
ATTAAAATTGGTTATATATGTGTTAATATTTAATTTCTTTGCAGGAACTATACTTTGTAA
ACGATAGAGGGATATACCTTGAACGGAATGTAATGCACTTTAGAAATAATAGGATTTTAG
CAGATGTAGATAATAAAACGAAAGACATAATTTATGGATATCGAAAAAAAAAAAATGAAG
TACAAAAAGAGTCTGATAATAAAAGGAATGGTGAATTAGGAATACAACCAAAACAAGATA
AAATAATAGCAAACAAAGATGAAAATAATTCTGCATCAGAACAAGAAAGCTTTAACCAAT
TGGAAAATAAAGGATATTTATCTGGGACGAAATATAATGAGACCGATTCAAGTAATAATC
ATAAAAAACTCCAAGCTGATCAAGAGTTAAGATTATGGGAAATAAAAGTATTTAAGAAGT
CTTTATTGCTGATAATAAGAGTTTTGGCGATATTAGTATCAAAAACAATAAAAATATTAT
TACTACTTCTACGGATTGTTTTTTTCATATTTAAGGTCTGGTGGGAATCCAGAAATAATC
GCTTTAAATTAAAAGGATCCGGTGGAGGTGGAAGATCCATGGTGAGCAAGGGCGAGGAGG
ATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGGGCTCCGTGA
ACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGGGCACCCAGA
CCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACATCCTGTCCC
CTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCCCCGACTACT
TGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCGAGGACGGCG
GCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCTACAAGGTGA
AGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGACCATGGGCT
GGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCGAGATCAAGC
AGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCACCTACAAGG
CCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGGACATCACCT
CCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCCGCCACTCCA
CCGGCGGCATGGACGAGCTGTACAAGTAATCTAGAATTATTAATATATATGAATATATAT
ACATCGTTGTATGCGTAATATACGTGTAACTGTTTTTTTTAATTTTTATATTAACTTTTC
CTTATACATTTTAATGCCATTAACTAATAATATTTTACTTGAAAAAATTCTAAATTTTTA
TAACTCTAATATAAAATCTGTGAATAATAAAAAATTAGCATAAAGTACGAATTAAACATC
GAATATTTGTTTTAAAGGGGCACACATTATGTGCATATAATTTTATTCTTTGAATTTAAT
TTTTTGCTTATAATTAGATTAAATTAATATAATTTTTTTATTGAAAATAAAAACTTATTT
TAAAAAATTAACAAATGGAAGTTAATGAAAAATACGAAAAAAGTAAAGGGTTAATTCTTA
TATGGTCGGTACCGAGCTCGAATTCGATATCGAAATTGAAGGAAAAAACATCATTTGTGT
TTATATGTTTTTTTTTAATTTTTCAACTTTTTTTATTTAACTATGCTAATCATATTATAA
ACATTTTAAAAATCAAGTCCAACTATTTATGAATCATTGAAGAGACAACATAATTATAAC
AGTAAAAATAATAATAATAATAATAATTTTGTCTTATTGTTTCAAACATATTATGTTCCA
TAAATATCTAAATTTTTAATATGGTCATAAATTCATAGGATTTAATTTATATAAAATATG
GCTATTCATACTAGCCATTTTATGTGTGTAAAGTTTTTTTTTTCTGAACAAAAAATAAAA
TGTTCATAATTTTAGCTATTTACATGCATGTGCATGCACAAAAAAAAATATGCACACAAC
ATACACATTTTTACAGTTATAAATACAATCAATTGGTATAAATATATAAGTAAGAAAAAC
GGGATCAAAGCTAGCTTCTGTATTTCCGCTAGACAGCCATCTCCATCTGGATTCGTCCGT
GCGGGACGTAGCCCACGACCTCAAAATCCTCGGCGGTGAAATCGTCGATTTCCTTGATGC
GTTCCTTGTTGAGGATGTTCACAATGGGGAACGGTCTCGGTTCTCTCCGCAGCTGCTCTT
TTAAAGCCTCGACATGGTTCGTGTAGACATGCGTGTTCCCCATGAAGTGAATGAACTCCT
TAGGTTTTAGGTTGCAGACGTGTGCAACCATGAGCGTCAAAAGCGAATAGGAAGCGATGT
TGAAGGGGACGCCGAGGCCGACATCGCACGACCGCTGATACATGATGCACGACAGCTCCT
TCTGGTCGTTCACGTAGAACTGGCACAACAAGTGACAAGGCGGCAGCGCCATTTCGTCCA
GCGCTGCAGGATTCCAGGCAGTCATGAGCATGCGACGATCTGTTGGATTCGTTCTCAGCA
TCTGGATCACATTCTTCAGCTGGTCGACGCCCTGCCCTGTGTAGTCTGTGTGCATGTCTT
TGTATGCCGCGCCGAAGTGTCTCCACTGGAAGCCGTAGCCCGGGCCGATGTCTCCGACCT
CTCGGTGGGGGAGATTGCGCGAATCGAGGAACTCGCGTGTCACATTCTTGTCCCAGATCT
TCACGCCCTTCTCAGAAAGATGGTTTGCGTTCGTGTCGCCGCGAATGAACCACAGCAACT
CTTCGAGGACCCCTTTCCAGAACACACGCTTTGTGGTGAGAAGTGGAAAGGCCTGATCCA
GCGAGTAGCGCATAGTGCAGCCGAATTTGGAGATGACACCAACGCCCGTTCGGTCATCCA
TTGTCCTTCCATTGTTAATAATGTCGGCAATGAGATCAAGGTACTGGAACTCTTCATGGC
CTCTAAAGTGAACATGCGGAACGGCCCGAATCAGTTCCTTTTGCTCGCGTTTTTTCCGGT
CTTCTTCGTCCATCCACGCCAACACCGGGGCAATGGCTGCGGCCGAAGAAGGAGCCTGCA
ACCCGTGCACGGGAGTTGTCTCCCTCGTGGACGTCAAGGAGCTCATTGCGTTGCTCGGTT
CCGCAGTGGCTGCGTCGTCAGTCTTCCTTCTCTTCTCGAGAACCACAAAGTCGTAGGGAA
CCCCGTTGTCTGAGAAGGTCTTGGAAATGAAGATGGGTCGATACGTCGCTTCATTGTCCT
TCTCTCTTCCGAGCTCCGGACAAAAGGGAACGAACACAGACTCGGCAGGAGCTGCAGCCT
GCGCAGCAGTTGATTTGTTTGAAAGAATGTCATCTCCGGGGAACGCAGGGAAGAAAACGT
CGCACGGAAACTCGCGGGCTACACGCGTGATGTACAGGTGAGAGGCAACGCCCAGAGACA
GCGCTGCCTCGTACAGTCCCGCTCCTCCCACGACAAAAATCTGGTCGACAGAATCCTTGT
ACTCTTCCTCCAGAAGGCTGAGAGCTGCTGGGAGTGAAGCACAGACTCGGACGCGCTGCT
GGCCTTCAGCTTGAGGCTTCTCCGCCGCAATGTCTTCTTCTTTGAGGGAAGAGGAAACGA
CGATGTTCAATCTGTCCACGAGGGGTCTAAACTTTCGAGGCATGCTTTCCCAGGTTTTCC
GTCCCATGACAACGGCGTTGAATCTCTTGCCGACTGATGGAGAGGGAAGTCCAGAGTCGC
CCGTCTTTGCAAATTTCCTGGGAAGCCACCCGTTCAGGCGACTGGCTTCTTCGGGCGTCG
TTTTTGTCACACGAGAAAAGTGTTTGAAATCTGTGGTCAAGTGGGGCCACGGGAGGCCGT
TGTTGATGCCGATGCCCCTCTTGGGGGTCATCGCGACGACCAGACACACCGGTTTATGCA
TTGATCCTTTGTAACATTTAGGTGTGTATTTATATATATAAGCAATATACAAATGAAAAT
ACTATTATAAAACGGAAACTCAAAAAATGTTGAAACAAATAGTCAATTTTTAAAATGAAT
GTGTAAATTGTTTAAGTTTTATGACAAAAAAAGGAAAAGTGTATATAAAATAAAAATTGG
AAAAAATTTATATATTACATGCCATATAATACCATTTATTTACTATTTATATCTTAATAT
AAAAAATTGTAGCAAAAATATATAATCGTATTTTATATATTTAATATTATATAACCCCTT
AAAAAAAAAAATTTAATTGAAAAAATTTTAATTTATGCACTTTTTTATTGTATTATATAT
ATTCCGTTATTTTAATTACACATAAACTTAATAGAAAAATTAATTCGCTGCTAAATAAAA
ATTATAGGGCTATATATGATGGGATCATATTCTATCTTTCTCTTTTTCAAATYCTTTATA
TTTTAGAATACAAATCGCTTTAAACAATAAAAAAGAGGTATATCCTACCCGAATTGACAC
ACACAATAAATAATAAATATATAAATAATAAAAATATAAATATATAAATTATAAATATAT
AAATTATATATATAAATATATTAACATGTGTGTAATTTGTTAAAATTGATATTAAACAGC
TTTGCATGGGGAAAGAGTGGCTTTTATAAGCGAATCATATAATACAGTGTTAATTTTAAT
ATATAAAATATAATTTCCTTAAATTGTTATTTAACATTTTTAATTGCCTACCCARGGTAA
TATAATATCCAAATGACAATCAAAGATAAATGAAATACCGCTCCATTTTTCCTATATATC
CATTTTCATTTTGTGTAAATGTTCATAAAATTTTATCATATACTCATAAAAAACAGGGGA
AAAATGAAAAAATGAACGTCCACCCCGTGTGAATATGCTCATTTTGTATACTATACATTT
TTTTTTATATAATTTATATTTTCCAAATAAAAAAAATGTTTGTACATGTATATATCAATA
TTGTGTTATGGCATGATGAAAAACAATTATTTTATTCGTTTTTAAATTTGTAGAATTTCC
CTAAATTATTAATGATTTCATATTATAATATATGTATCTATATATTTTTAATGTGTAATT
CAAAATGGCGAAAAAGAAACAAACCATACTTTATAAGCAACTCTCTTTGAGCACTTACCA
TAAAAAATTATACTACTAAATTCAAATTAAATAAAAAAATATATATTATAAAATTGGCTT
TAATTATTTATAAGCACTTGCTTTGTTTTACTCAAAAAGTATTGGCATAAGTATAAACAT
AAAACCAATTTTTCACAAAATATATTATATGTCCATACAACTATATCCGAACYTTCAAAA
CACATCCAAATTTCCATTTACCACATGCAAGCAAAAGGATATTAAATAAAAAAAAATTAT
TTATATTTTCATGATCACTTAAATAATATTTAAAAAAAAAAGAGACCATATATAAAATGT
ATAATTTAAATATAGCTAACTTATTAAAAAAAATGAATATTGAGCATATATCCATAATTT
AAAACACTTCTATTTTATGTTATTTGATATATAAGTCGATAAATTATTGCATGTATGCAT
ATAGACAGATTAATATACACCAAAATAATAAAAATTATATATAGTTCATATATTATTCTG
AATAATAAATTAGCTTTCCTCTTAATTCACTTGGTATGTATATATATATATATATATATA
TATATATATATATGGATAAATAAATATAAGGTCTATAACAGGAAAAAATGTGTATTAACT
TTTTTTTATAATTATTTTATATTTTTTCTCCATTTATTTAAATATGTTAAAATTATTTCC
CATTTTATAAACAAAAATTAATCCATAAAATAGCAAAATAACAAAATGACAAAATAACAA
AAACAAAAAAACGCATTATATGAGTTCATTTTACACAATCCAAAGAAAAAGAAAATATGC
TTAAAATATTCATAACACACTTTTAAGCTATAAAAATATATATATTTATTTGTGTCTATA
TTACCAACTCAATTTAATAGATGTGTTATGTGATTAATTCATACACAAACATACAAAAAT
AAACACCATTTTTGAAAAGATTAATTTGAAATCATATATGTTATTATTATATATTTTTAT
ATCAATGAAATTTAAAAAAATAAAAGGGAAATCAATGTATTAAAAATAATTATATGTTAT
TTTATTTCCACAATATTTATTTATTATTTATTGTTGACCTGCAGGCATGCAAGCTTATCG
ATACCGTCGACCTCGAGGGGGGGCCCGGTACCCAGCTTTTGTTCCCTTTAGTGAGGGTTA
ATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTC
ACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGA
GTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTG
TCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGG
CGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCG
GTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGA
AAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTG
GCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAG
AGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTC
GTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCG
GGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTT
CGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCC
GGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCC
ACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGG
TGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCA
GTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGC
GGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGAT
CCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATT
TTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGT
TTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATC
AGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCC
GTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATA
CCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGG
GCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGC
CGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCT
ACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAA
CGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGT
CCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCA
CTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTAC
TCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCA
ATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGT
TCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCC
ACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCA
AAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATA
CTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGC
GGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCC
CGAAAAGTGCCAC
Restriction sites to linearize plasmid SpeI
Selectable marker used to select the mutant parasitetgdhfr
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1CCCCGCGGCTATATGGTGAAATTCTCTATTATAAC
Additional information primer 1L5430 (SacII); PBANKA_021540 Forward
Sequence Primer 2CCGGATCCTTTTAATTTAAAGCGATTATTTCTGGATTC
Additional information primer 2L5491 (BamHI); PBANKA_021540 Reverse
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6