Summary

RMgm-696
Malaria parasiteP. berghei
Genotype
TaggedGene model (rodent): PBANKA_0837161; Gene model (P.falciparum): Not available; Gene product: BIR protein PIR protein
Name tag: GFP
Phenotype Asexual bloodstage;
Last modified: 2 December 2012, 15:28
  *RMgm-696
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene tagging
Reference (PubMed-PMID number) Reference 1 (PMID number) : 23197789
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone P. berghei ANKA cl15cy1
Other information parent lineA reference wild type clone from the ANKA strain of P. berghei (PubMed: PMID: 17406255).
The mutant parasite was generated by
Name PI/ResearcherE.M. Pasini, C.J. Janse, B.M.D. Franke-Fayard
Name Group/DepartmentDepartment of Parasitology
Name InstituteBiomedical Primate Research Centre
CityRijswijk
CountryThe Netherlands
Name of the mutant parasite
RMgm numberRMgm-696
Principal name603cl3
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationYes
Phenotype
Asexual blood stageThe tagged-protein is exported into the cytoplasm of the host erythrocyte and shows a diffuse or patchy localization in the cytoplasm
Gametocyte/GameteNot different from wild type
Fertilization and ookineteNot different from wild type
OocystNot different from wild type
SporozoiteNot different from wild type
Liver stageNot different from wild type
Additional remarks phenotype

Mutant/mutation

Protein (function)

Phenotype

Additional information
The mutant parasite expresses an GFP-tagged protein. The protein has been selected for tagging in a screen for putative exported proteins of P. berghei.

The genotype of the parasites has not been analysed in detail.
Southern analysis of PFG-separated chromosomes (to show integration into the chromosome on which the target gene is located) has been performed (see below). This analysis provided evidence for tagging of the correct gene. The construct used aims at integration of the tagging construct by single-cross-over integration resulting in the presence of a tagged copy of the endogenous gene.

Phenotype analyses of the blood stages by fluorescence microscopy show that the tagged-protein is exported into the cytoplasm of the host erythrocyte and shows a diffuse or patchy localization in the cytoplasm.



Other mutants
RMgm-221: mutant expressing a GFP-tagged form of PBANKA_000320


  Tagged: Mutant parasite with a tagged gene
Details of the target gene
Gene Model of Rodent Parasite PBANKA_0837161
Gene Model P. falciparum ortholog Not available
Gene productBIR protein PIR protein
Gene product: Alternative name
Details of the genetic modification
Name of the tagGFP
Details of taggingC-terminal
Additional remarks: tagging
Commercial source of tag-antibodiesRoche
Type of plasmid/constructPlasmid single cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
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Plasmid/construct sequence
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CATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGA
CGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTA
CGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCAC
CCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAA
GCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTT
CTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCT
GGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCA
CAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAA
CGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGC
CGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCA
CTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGT
CCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTA
AAGCGGCCGCGACTCTAGAAGATCCCGTTTTTCTTACTTATATATTTATACCAATTGATT
GTATTTATAACTGTAAAAATGTGTATGTTGTGTGCATATTTTTTTTTGTGCATGCACATG
CATGTAAATAGCTAAAATTATGAACATTTTATTTTTTGTTCAGAAAAAAAAAACTTTACA
CACATAAAATGGCTAGTATGAATAGCCATATTTTATATAAATTAAATCCTATGAATTTAT
GACCATATTAAAAATTTAGATATTTATGGAACATAATATGTTTGAAACAATAAGACAAAA
TTATTATTATTATTATTATTTTTACTGTTATAATTATGTTGTCTCTTCAATGATTCATAA
ATAGTTGGACTTGATTTTTAAAATGTTTATAATATGATTAGCATAGTTAAATAAAAAAAG
TTGAAAAATTAAAAAAAAACATATAAACACAAATGATGTTTTTTCCTTCAATTTCGGGTA
CCGAGCTCGAATTCAACCTGGTTGATCTTGCCAGTAGTCATATGCTTGTCTCAAAGATTA
AGCCATGCAAGTGAAAGTATATGCACATTTATTGCAGAAACTGCGAACGGCTCATTAAAA
CAGTTATAATCTACTTGACATTTTATTATAAGGATAACTACGGAAAAGCTGTAGCTAATA
CTTGTTAAGTACTTTTACTCCCCGGAGTAATTGTATGTATTTGTTAAGACCCCTAAGAAA
AAATGATATTAAAGGAATTATAACAAAGAAGCAACACATAATATAATTATTCAGTGTGTA
TCAATCGAGTTTCTGACCTATCAGCTTTTGATGTTAGGGTATTGACCTAACATGGCTTTG
ACGGGTAACGGGGAATTAGAGTTCGATTCCGGAGAGGGAGCCTGAGAAATAGCTACCACA
TCTAAGGAAGGCAGCAGGCGCGTAAATTACCCAATTCTAAATAAGAGAGGTAGTGACAAG
AAATAACAATATAAGGCCAAATTTTGGTTTTATAATTGGAATGATGGGAATTTAAAACCT
TCCCAAAAATCAATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTGGAGCTCCAATTCG
CCCTATAGTGAGTCGTATTACGCGCGCTCACTGGCCGTCGTTTTACAACGTCGTGACTGG
GAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGG
CGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGC
GAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGC
GTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTT
CTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTC
CGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGT
AGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTT
AATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTT
GATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAA
AAATTTAACGCGAATTTTAACAAAATATTAACGCTTACAATTTAGGTGGCACTTTTCGGG
GAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGC
TCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTA
TTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTG
CTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGG
GTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAAC
GTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTG
ACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGT
ACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTG
CTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGAC
CGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTT
GGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAG
CAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGC
AACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCC
TTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTA
TCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGG
GGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGA
TTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAAC
TTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAA
TCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGAT
CTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGC
TACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTG
GCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACC
ACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGG
CTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGG
ATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAA
CGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCG
AAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGA
GGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCT
GACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCA
GCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTC
CTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCG
CTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCC
CAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACA
GGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTC
ATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGA
GCGGTAAACAATTTCACACAGGAAACAGCTTGACCATGATTACGCCAAGCTTGCATGCCT
GCAGGTCAACAATAAATAATAAATAAATATTGTGGAAATAAAATAACATATAATTATTTT
TAATACATTGATTTCCCTTTTATTTTTTTAAATTTCATTGATATAAAAATATATAATAAT
AACATATATGATTTCAAATTAATCTTTTCAAAAATGGTGTTTATTTTTGTATGTTTGTGT
ATGAATTAATCACATAACACATCTATTAAATTGAGTTGGTAATATAGACACAAATAAATA
TATATATTTTTATAGCTTAAAAGTGTGTTATGAATATTTTAAGCATATTTTCTTTTTCTT
TGGATTGTGTAAAATGAACTCATATAATGCGTTTTTTTGTTTTTGTTATTTTGTCATTTT
GTTATTTTGCTATTTTATGGATTAATTTTTGTTTATAAAATGGGAAATAATTTTAACATA
TTTAAATAAATGGAGAAAAAATATAAAATAATTATAAAAAAAAGTTAATACACATTTTTT
CCTGTTATAGACCTTATATTTATTTATCCATATATATATATATATATATATATATATATA
TATACATACCAAGTGAATTAAGAGGAAAGCTAATTTATTATTCAGAATAATATATGAACT
ATATATAATTTTTATTATTTTGGTGTATATTAATCTGTCTATATGCATACATGCAATAAT
TTATCGACTTATATATCAAATAACATAAAATAGAAGTGTTTTAAATTATGGATATATGCT
CAATATTCATTTTTTTTAATAAGTTAGCTATATTTAAATTATACATTTTATATATGGTCT
CTTTTTTTTTTAAATATTATTTAAGTGATCATGAAAATATAAATAATTTTTTTTTATTTA
ATATCCTTTTGCTTGCATGTGGTAAATGGAAATTTGGATGTGTTTTGAARGTTCGGATAT
AGTTGTATGGACATATAATATATTTTGTGAAAAATTGGTTTTATGTTTATACTTATGCCA
ATACTTTTTGAGTAAAACAAAGCAAGTGCTTATAAATAATTAAAGCCAATTTTATAATAT
ATATTTTTTTATTTAATTTGAATTTAGTAGTATAATTTTTTATGGTAAGTGCTCAAAGAG
AGTTGCTTATAAAGTATGGTTTGTTTCTTTTTCGCCATTTTGAATTACACATTAAAAATA
TATAGATACATATATTATAATATGAAATCATTAATAATTTAGGGAAATTCTACAAATTTA
AAAACGAATAAAATAATTGTTTTTCATCATGCCATAACACAATATTGATATATACATGTA
CAAACATTTTTTTTATTTGGAAAATATAAATTATATAAAAAAAAATGTATAGTATACAAA
ATGAGCATATTCACACGGGGTGGACGTTCATTTTTTCATTTTTCCCCTGTTTTTTATGAG
TATATGATAAAATTTTATGAACATTTACACAAAATGAAAATGGATATATAGGAAAAATGG
AGCGGTATTTCATTTATCTTTGATTGTCATTTGGATATTATATTACCYTGGGTAGGCAAT
TAAAAATGTTAAATAACAATTTAAGGAAATTATATTTTATATATTAAAATTAACACTGTA
TTATATGATTCGCTTATAAAAGCCACTCTTTCCCCATGCAAAGCTGTTTAATATCAATTT
TAACAAATTACACACATGTTAATATATTTATATATATAATTTATATATTTATAATTTATA
TATTTATATTTTTATTATTTATATATTTATTATTTATTGTGTGTGTCAATTCGGGTAGGA
TATACCTCTTTTTTATTGTTTAAAGCGATTTGTATTCTAAAATATAAAGRATTTGAAAAA
GAGAAAGATAGAATATGATCCCATCATATATAGCCCTATAATTTTTATTTAGCAGCGAAT
TAATTTTTCTATTAAGTTTATGTGTAATTAAAATAACGGAATATATATAATACAATAAAA
AAGTGCATAAATTAAAATTTTTTCAATTAAATTTTTTTTTTTAAGGGGTTATATAATATT
AAATATATAAAATACGATTATATATTTTTGCTACAATTTTTTATATTAAGATATAAATAG
TAAATAAATGGTATTATATGGCATGTAATATATAAATTTTTTCCAATTTTTATTTTATAT
ACACTTTTCCTTTTTTTGTCATAAAACTTAAACAATTTACACATTCATTTTAAAAATTGA
CTATTTGTTTCAACATTTTTTGAGTTTCCGTTTTATAATAGTATTTTCATTTGTATATTG
CTTATATATATAAATACACACCTAAATGTTACAAAGGATCAATGCATAAACCGGTGTGTC
TGGTCGTCGCGATGACCCCCAAGAGGGGCATCGGCATCAACAACGGCCTCCCGTGGCCCC
ACTTGACCACAGATTTCAAACACTTTTCTCGTGTGACAAAAACGACGCCCGAAGAAGCCA
GTCGCCTGAACGGGTGGCTTCCCAGGAAATTTGCAAAGACGGGCGACTCTGGACTTCCCT
CTCCATCAGTCGGCAAGAGATTCAACGCCGTTGTCATGGGACGGAAAACCTGGGAAAGCA
TGCCTCGAAAGTTTAGACCCCTCGTGGACAGATTGAACATCGTCGTTTCCTCTTCCCTCA
AAGAAGAAGACATTGCGGCGGAGAAGCCTCAAGCTGAAGGCCAGCAGCGCGTCCGAGTCT
GTGCTTCACTCCCAGCAGCTCTCAGCCTTCTGGAGGAAGAGTACAAGGATTCTGTCGACC
AGATTTTTGTCGTGGGAGGAGCGGGACTGTACGAGGCAGCGCTGTCTCTGGGCGTTGCCT
CTCACCTGTACATCACGCGTGTAGCCCGCGAGTTTCCGTGCGACGTTTTCTTCCCTGCGT
TCCCCGGAGATGACATTCTTTCAAACAAATCAACTGCTGCGCAGGCTGCAGCTCCTGCCG
AGTCTGTGTTCGTTCCCTTTTGTCCGGAGCTCGGAAGAGAGAAGGACAATGAAGCGACGT
ATCGACCCATCTTCATTTCCAAGACCTTCTCAGACAACGGGGTTCCCTACGACTTTGTGG
TTCTCGAGAAGAGAAGGAAGACTGACGACGCAGCCACTGCGGAACCGAGCAACGCAATGA
GCTCCTTGACGTCCACGAGGGAGACAACTCCCGTGCACGGGTTGCAGGCTCCTTCTTCGG
CCGCAGCCATTGCCCCGGTGTTGGCGTGGATGGACGAAGAAGACCGGAAAAAACGCGAGC
AAAAGGAACTGATTCGGGCCGTTCCGCATGTTCACTTTAGAGGCCATGAAGAGTTCCAGT
ACCTTGATCTCATTGCCGACATTATTAACAATGGAAGGACAATGGATGACCGAACGGGCG
TTGGTGTCATCTCCAAATTCGGCTGCACTATGCGCTACTCGCTGGATCAGGCCTTTCCAC
TTCTCACCACAAAGCGTGTGTTCTGGAAAGGGGTCCTCGAAGAGTTGCTGTGGTTCATTC
GCGGCGACACGAACGCAAACCATCTTTCTGAGAAGGGCGTGAAGATCTGGGACAAGAATG
TGACACGCGAGTTCCTCGATTCGCGCAATCTCCCCCACCGAGAGGTCGGAGACATCGGCC
CGGGCTACGGCTTCCAGTGGAGACACTTCGGCGCGGCATACAAAGACATGCACACAGACT
ACACAGGGCAGGGCGTCGACCAGCTGAAGAATGTGATCCAGATGCTGAGAACGAATCCAA
CAGATCGTCGCATGCTCATGACTGCCTGGAATCCTGCAGCGCTGGACGAAATGGCGCTGC
CGCCTTGTCACTTGTTGTGCCAGTTCTACGTGAACGACCAGAAGGAGCTGTCGTGCATCA
TGTATCAGCGGTCGTGCGATGTCGGCCTCGGCGTCCCCTTCAACATCGCTTCCTATTCGC
TTTTGACGCTCATGGTTGCACACGTCTGCAACCTAAAACCTAAGGAGTTCATTCACTTCA
TGGGGAACACGCATGTCTACACGAACCATGTCGAGGCTTTAAAAGAGCAGCTGCGGAGAG
AACCGAGACCGTTCCCCATTGTGAACATCCTCAACAAGGAACGCATCAAGGAAATCGACG
ATTTCACCGCCGAGGATTTTGAGGTCGTGGGCTACGTCCCGCACGGACGAATCCAGATGG
AGATGGCTGTCTAGCGGAAATACAGAAGCTAGCTTTGATCCCGTTTTTCTTACTTATATA
TTTATACCAATTGATTGTATTTATAACTGTAAAAATGTGTATGTTGTGTGCATATTTTTT
TTTGTGCATGCACATGCATGTAAATAGCTAAAATTATGAACATTTTATTTTTTGTTCAGA
AAAAAAAAACTTTACACACATAAAATGGCTAGTATGAATAGCCATATTTTATATAAATTA
AATCCTATGAATTTATGACCATATTAAAAATTTAGATATTTATGGAACATAATATGTTTG
AAACAATAAGACAAAATTATTATTATTATTATTATTTTTACTGTTATAATTATGTTGTCT
CTTCAATGATTCATAAATAGTTGGACTTGATTTTTAAAATGTTTATAATATGATTAGCAT
AGTTAAATAAAAAAAGTTGAAAAATTAAAAAAAAACATATAAACACAAATGATGTTTTTT
CCTTCAATTTCGATCTGAAACGCGGGAATATTATACATTGATTTAAAGTATTTTCATTAA
GCGCACTAATTATTCCGACGTACTATACAATGATATAGCACTGACAATAGATAAACGTAT
TAAATACGAACAAATCATTAAATTAATTTGATTCGGATACATTGATATATATTATTAAGC
TTGTAATAAGATTAAAATTATATGCGCAAAACATCAAATGAAATATTATAACACTTATTT
AAGTAAGATTTAATGCGATGACATTAGACTAACACTATCAAGCAAAATAATATTAATAAT
TATAGTCTTCTTAAAAATAATTTTTTTCATCATAAAACTAAATATAGTTTATTATAAAAT
ATAAGAGCAAATTATATATTTAGAAAATAATTCTAAGCCATTTTTAATTTATACATTAAT
TGAAAGTTTGAATGGTAAAAAATATAATTAAACTATGTAGAATATGTAAATCTTATATGG
ATACATCATTGTCTTAAAAAAGCATAATTCCCTTATCTCTCCTCTCAAAGTGCAATATAC
CAACCTAATTACGCAAAGTTTTCTATTATACTTAAAAATTTACATCAATACTTATTTGTT
TATTAATATTTAATATTTACTAAGTATTATTTTAAAAACAATATACATTAAAAGACTTAT
TTAAGCTTATAAATAACATAGTAAATACAGTTTCAGTGCTAATTCTCGTTTTAAACCGTG
GAAAAGATGTGCAAAATATATTAAAAATTATCCAATAAGGAATATTTCTAAATTAAAGTA
TATAGGAATACAAATAATGTTATAAGACTCATTAAAATGAATTATTAATTTATCTACATT
AAATAAAAATAATATTATAATTATGTAATTTGCATAGAAAATGGAACATTCAACTTAATT
TGTAAATGTTATAATTTTAATAAAATATTGGTATATATTATAAGAAACAAGTATATAAAT
ATTTAATATATTTTAAAAAAATTATATTTATATACTTTTAAAGATTATAGTAATAATAAC
TTTTTTAATTTTTTATATTTTTGCAGTATTTAAGTTTTAGGATATTGTCTATGTTCTATA
TTAAATCATATGTATAAGAAAATATATTATAAATTTATTATAAAAGTATATTAATTTTTA
TAATAATGCTCATATGAGTGTTATTAAGTATAAAAATTTATTCAAAATTGTATTATATAT
ACATATGATAAAACATGTATTAAACACCCTAAAACAAGGTAATTTATCTAACTATCATAA
CAAAAATATAATATTCTTTTAGTAATAATATTATATTATTATTACATATTAATTTTAAAT
TGAAATTAATAATAATAAAAATATTTTTATCTACAGATAATTGCTATATATAGGGTTAAA
TAATTGTATTACCTATTTTAGTATATATATAATATGATATCCCCTTAACCTAGAAATCAT
AAATTATATTACATCACAATGGATGATGCTCTAGTATATACACTTTTTTATATTATATTT
CCTATAAACTTCATTAAGTTTTATTTTAATATCATTTTCTTAATACATATTTTTACCATT
TTTTTTAAATGGTTTCTTAGTGTCAAAACTTTGATGTTTTGATGAAGTATTTACCTGCTG
AATTAGGCGGAACCGCAAATTTTGAATTTAAACAAATTACAAATTTCGATAAGTACTGCC
CTAATGGAGACTGCAATACTAATCTCGAAAAAATTACAATTGGATTTTTATGGTTACTTG
AACAATGTTTTTCTACACCCATAAATAAAAATCATAATGAAAACAATACTAATGCATATT
TTATATATGTTGTATTATGGTTAAGTTACAAATTAAATCAAATAACAGGCCAAAATTTCA
CTAAAATAAACGATTTTTATACTAATCAAGTAAATAATAGTGGTAAATATAGTAAATTTA
TAAGTGATTCCAATAGATATACAAATATTAAGGAATTCATAGAGAAACAAAAAGATTTGT
TGAATATTAATATTGAAGATCTTTCTAAATTTTATGATGCATCCAAATTAATATGTAGTA
TGTATGGTAATTATGCAATGAATAATAATAACAAACTGTCAAATAATGCTACTAGTTTTG
TTAACACATATACAGAGCTTAACAATAACTATAATACTAAAGGTACCGCATATAGTCAAA
TACTGTCTGCCTTATCAACTGATTATAATAATTTAAAAAATAAATGTAGCAATATTCCAA
CCCTTTCAGAGATAAACACATCAAAAAATACTGTAGAAACTTCTGATCAAAGTCCTGAAC
AAAGTGTACAAATATTAGAACATATTTCTGAAGTTACATCATCAAGTTCGTCGATAGGAA
ACAAATTATTTACAGTTTTATCGATATTTGGTGCAATAGCATTTTTTTTAGGAATTTCTT
ATAAGGTAAATAATAAGGAATTAAAAAAATATTTTCATTATATATATGCAAGCATTAACA
AAAATACCATACGCTTCTTAACGTTTTATATTAGTATTCGTTATTTGGACGTCGGAAACG
ATCTCAAAAACAATATTTAAGAGAAAAAATAAAAAATATAAAGAAGAGAATGAATCATCC
Restriction sites to linearize plasmid EcoRV
Selectable marker used to select the mutant parasitetgdhfr
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1TTCCAGCTGGAAACGCGGGAATATTATAC
Additional information primer 1L2197 (PvuII); PBANKA_000320 Forward
Sequence Primer 2CATGCCATGGGATGATTCATTCTCTTC
Additional information primer 2L1906 (NcoI); PBANKA_000320 Reverse
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6