Summary

RMgm-693
Malaria parasiteP. berghei
Genotype
TaggedGene model (rodent): PBANKA_1327300; Gene model (P.falciparum): Not available; Gene product: fam-a protein
Name tag: mCherry
Phenotype Asexual bloodstage; Liver stage;
Last modified: 16 August 2015, 12:21
  *RMgm-693
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene tagging
Reference (PubMed-PMID number) Reference 1 (PMID number) : 23197789
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone P. berghei ANKA cl15cy1
Other information parent lineA reference wild type clone from the ANKA strain of P. berghei (PubMed: PMID: 17406255).
The mutant parasite was generated by
Name PI/ResearcherE.M. Pasini, C.J. Janse, B.M.D. Franke-Fayard
Name Group/DepartmentDepartment of Parasitology
Name InstituteBiomedical Primate Research Centre
CityRijswijk
CountryThe Netherlands
Name of the mutant parasite
RMgm numberRMgm-693
Principal name1448cl2
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationYes
Phenotype
Asexual blood stageThe tagged-protein is exported into the cytoplasm of the host erythrocyte and shows a diffuse or patchy localization in the cytoplasm
Gametocyte/GameteNot tested
Fertilization and ookineteNot tested
OocystNot different from wild type
SporozoiteNot different from wild type
Liver stageThe tagged protein is expressed in late liver stages (schizonts) where it is located in the parasitophorous vacuole.
Additional remarks phenotype

Mutant/mutation

Protein (function)

Phenotype

Additional information

The mutant parasite expresses an mCherry-tagged protein. The protein has been selected for tagging in a screen for putative exported proteins of P. berghei.

The genotype of the parasites has not been analysed in detail. Southern analysis of PFG-separated chromosomes (to show integration into the chromosome on which the target gene is located) has been performed (see below). This analysis provided evidence for tagging of the correct gene. The construct used aims at integration of the tagging construct by single-cross-over integration resulting in the presence of a tagged copy of the endogenous gene.

Phenotype analyses of the blood stages by fluorescence microscopy show that the tagged-protein is exported into the cytoplasm of the host erythrocyte and shows a diffuse or patchy localization in the cytoplasm.

 

The protein is not detected in oocysts and in sporozoites.

The tagged protein is expressed in late liver stages (schizonts) where it is located in the parasitophorous vacuole.





Other mutants
See the link for other mutants


  Tagged: Mutant parasite with a tagged gene
Details of the target gene
Gene Model of Rodent Parasite PBANKA_1327300
Gene Model P. falciparum ortholog Not available
Gene productfam-a protein
Gene product: Alternative name
Details of the genetic modification
Name of the tagmCherry
Details of taggingC-terminal
Additional remarks: tagging
Commercial source of tag-antibodiesClontech
Type of plasmid/construct(Linear) plasmid single cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
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Plasmid/construct sequence
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AACATATTATGGGACCCCAATGGCGCAAAGAACTTCGATGATAAATTTATTAAAGGTATA
TAATTAATTTATTTATGTGTTTATCAATCTTTATTATGCATTTCTTGTTTTTTTAAATTT
AATAATGTTTTAATTTGCATGTATATACAATTACAATTTTTTTAGGAACCATTCGTCGAG
TATACAATAAAAATTTAGTAATTATACAACAACTCTACAAAAGTCTTATTAGATCATGGC
AAAGATATTATCATGCACTAGCCAACAAAATTGAAGTAAGGAAATTTTTATTTTTTTTCA
TCCTTCTAGATATACGTTTTTTCATATTATTATATCCTAATTTAAGAAAATATTCTTTTT
ATTAATTTATAGTTATCAAAAAACAAAACTGCAATAGTCTTGGTTTCATCAGACATGAAT
GATCATGATGGTGGAAAAAATAAAAAATATGTAAACCCTTTCGTAGAAAGTGCAAACTCA
TTCAAGCCTGACATAGATTCTGAAGAAGATATTAGAAATGGAGATTTATACAAAATGTAT
ATTAACTTAGTAGCATTTTTTATTGAAAAGGAAGCCGATTGCGTTAAAGTTACCTATGTC
AGCTCTGTAAGCAATATAAAAATTTTAATAGCATAATTTTATTTCACAATTATCAAAAAA
ATATAATTTCTATATAAATGTGCATATTTACAAATATCTATTAATTCACAATGAAAATGG
AGCATTTTATATATTTATTATTTTTTTGTTTTTTTTCTCAGATTGATCCAAATGCTCCTT
ACCTTACACCAGCAAGTTTTATTAAAAAAATTATAGTCAAAAAAATTTTAACTCTTGTAA
AATTAAAGGATATTTTTAAAAAGGGATCCGGTGGAGGTGGAAGATCCATGGTGAGCAAGG
GCGAGGAGGATAACATGGCCATCATCAAGGAGTTCATGCGCTTCAAGGTGCACATGGAGG
GCTCCGTGAACGGCCACGAGTTCGAGATCGAGGGCGAGGGCGAGGGCCGCCCCTACGAGG
GCACCCAGACCGCCAAGCTGAAGGTGACCAAGGGTGGCCCCCTGCCCTTCGCCTGGGACA
TCCTGTCCCCTCAGTTCATGTACGGCTCCAAGGCCTACGTGAAGCACCCCGCCGACATCC
CCGACTACTTGAAGCTGTCCTTCCCCGAGGGCTTCAAGTGGGAGCGCGTGATGAACTTCG
AGGACGGCGGCGTGGTGACCGTGACCCAGGACTCCTCCCTGCAGGACGGCGAGTTCATCT
ACAAGGTGAAGCTGCGCGGCACCAACTTCCCCTCCGACGGCCCCGTAATGCAGAAGAAGA
CCATGGGCTGGGAGGCCTCCTCCGAGCGGATGTACCCCGAGGACGGCGCCCTGAAGGGCG
AGATCAAGCAGAGGCTGAAGCTGAAGGACGGCGGCCACTACGACGCTGAGGTCAAGACCA
CCTACAAGGCCAAGAAGCCCGTGCAGCTGCCCGGCGCCTACAACGTCAACATCAAGTTGG
ACATCACCTCCCACAACGAGGACTACACCATCGTGGAACAGTACGAACGCGCCGAGGGCC
GCCACTCCACCGGCGGCATGGACGAGCTGTACAAGTAATCTAGAATTATTAATATATATG
AATATATATACATCGTTGTATGCGTAATATACGTGTAACTGTTTTTTTTAATTTTTATAT
TAACTTTTCCTTATACATTTTAATGCCATTAACTAATAATATTTTACTTGAAAAAATTCT
AAATTTTTATAACTCTAATATAAAATCTGTGAATAATAAAAAATTAGCATAAAGTACGAA
TTAAACATCGAATATTTGTTTTAAAGGGGCACACATTATGTGCATATAATTTTATTCTTT
GAATTTAATTTTTTGCTTATAATTAGATTAAATTAATATAATTTTTTTATTGAAAATAAA
AACTTATTTTAAAAAATTAACAAATGGAAGTTAATGAAAAATACGAAAAAAGTAAAGGGT
TAATTCTTATATGGTCGGTACCGAGCTCGAATTCGATATCGAAATTGAAGGAAAAAACAT
CATTTGTGTTTATATGTTTTTTTTTAATTTTTCAACTTTTTTTATTTAACTATGCTAATC
ATATTATAAACATTTTAAAAATCAAGTCCAACTATTTATGAATCATTGAAGAGACAACAT
AATTATAACAGTAAAAATAATAATAATAATAATAATTTTGTCTTATTGTTTCAAACATAT
TATGTTCCATAAATATCTAAATTTTTAATATGGTCATAAATTCATAGGATTTAATTTATA
TAAAATATGGCTATTCATACTAGCCATTTTATGTGTGTAAAGTTTTTTTTTTCTGAACAA
AAAATAAAATGTTCATAATTTTAGCTATTTACATGCATGTGCATGCACAAAAAAAAATAT
GCACACAACATACACATTTTTACAGTTATAAATACAATCAATTGGTATAAATATATAAGT
AAGAAAAACGGGATCAAAGCTAGCTTCTGTATTTCCGCTAGACAGCCATCTCCATCTGGA
TTCGTCCGTGCGGGACGTAGCCCACGACCTCAAAATCCTCGGCGGTGAAATCGTCGATTT
CCTTGATGCGTTCCTTGTTGAGGATGTTCACAATGGGGAACGGTCTCGGTTCTCTCCGCA
GCTGCTCTTTTAAAGCCTCGACATGGTTCGTGTAGACATGCGTGTTCCCCATGAAGTGAA
TGAACTCCTTAGGTTTTAGGTTGCAGACGTGTGCAACCATGAGCGTCAAAAGCGAATAGG
AAGCGATGTTGAAGGGGACGCCGAGGCCGACATCGCACGACCGCTGATACATGATGCACG
ACAGCTCCTTCTGGTCGTTCACGTAGAACTGGCACAACAAGTGACAAGGCGGCAGCGCCA
TTTCGTCCAGCGCTGCAGGATTCCAGGCAGTCATGAGCATGCGACGATCTGTTGGATTCG
TTCTCAGCATCTGGATCACATTCTTCAGCTGGTCGACGCCCTGCCCTGTGTAGTCTGTGT
GCATGTCTTTGTATGCCGCGCCGAAGTGTCTCCACTGGAAGCCGTAGCCCGGGCCGATGT
CTCCGACCTCTCGGTGGGGGAGATTGCGCGAATCGAGGAACTCGCGTGTCACATTCTTGT
CCCAGATCTTCACGCCCTTCTCAGAAAGATGGTTTGCGTTCGTGTCGCCGCGAATGAACC
ACAGCAACTCTTCGAGGACCCCTTTCCAGAACACACGCTTTGTGGTGAGAAGTGGAAAGG
CCTGATCCAGCGAGTAGCGCATAGTGCAGCCGAATTTGGAGATGACACCAACGCCCGTTC
GGTCATCCATTGTCCTTCCATTGTTAATAATGTCGGCAATGAGATCAAGGTACTGGAACT
CTTCATGGCCTCTAAAGTGAACATGCGGAACGGCCCGAATCAGTTCCTTTTGCTCGCGTT
TTTTCCGGTCTTCTTCGTCCATCCACGCCAACACCGGGGCAATGGCTGCGGCCGAAGAAG
GAGCCTGCAACCCGTGCACGGGAGTTGTCTCCCTCGTGGACGTCAAGGAGCTCATTGCGT
TGCTCGGTTCCGCAGTGGCTGCGTCGTCAGTCTTCCTTCTCTTCTCGAGAACCACAAAGT
CGTAGGGAACCCCGTTGTCTGAGAAGGTCTTGGAAATGAAGATGGGTCGATACGTCGCTT
CATTGTCCTTCTCTCTTCCGAGCTCCGGACAAAAGGGAACGAACACAGACTCGGCAGGAG
CTGCAGCCTGCGCAGCAGTTGATTTGTTTGAAAGAATGTCATCTCCGGGGAACGCAGGGA
AGAAAACGTCGCACGGAAACTCGCGGGCTACACGCGTGATGTACAGGTGAGAGGCAACGC
CCAGAGACAGCGCTGCCTCGTACAGTCCCGCTCCTCCCACGACAAAAATCTGGTCGACAG
AATCCTTGTACTCTTCCTCCAGAAGGCTGAGAGCTGCTGGGAGTGAAGCACAGACTCGGA
CGCGCTGCTGGCCTTCAGCTTGAGGCTTCTCCGCCGCAATGTCTTCTTCTTTGAGGGAAG
AGGAAACGACGATGTTCAATCTGTCCACGAGGGGTCTAAACTTTCGAGGCATGCTTTCCC
AGGTTTTCCGTCCCATGACAACGGCGTTGAATCTCTTGCCGACTGATGGAGAGGGAAGTC
CAGAGTCGCCCGTCTTTGCAAATTTCCTGGGAAGCCACCCGTTCAGGCGACTGGCTTCTT
CGGGCGTCGTTTTTGTCACACGAGAAAAGTGTTTGAAATCTGTGGTCAAGTGGGGCCACG
GGAGGCCGTTGTTGATGCCGATGCCCCTCTTGGGGGTCATCGCGACGACCAGACACACCG
GTTTATGCATTGATCCTTTGTAACATTTAGGTGTGTATTTATATATATAAGCAATATACA
AATGAAAATACTATTATAAAACGGAAACTCAAAAAATGTTGAAACAAATAGTCAATTTTT
AAAATGAATGTGTAAATTGTTTAAGTTTTATGACAAAAAAAGGAAAAGTGTATATAAAAT
AAAAATTGGAAAAAATTTATATATTACATGCCATATAATACCATTTATTTACTATTTATA
TCTTAATATAAAAAATTGTAGCAAAAATATATAATCGTATTTTATATATTTAATATTATA
TAACCCCTTAAAAAAAAAAATTTAATTGAAAAAATTTTAATTTATGCACTTTTTTATTGT
ATTATATATATTCCGTTATTTTAATTACACATAAACTTAATAGAAAAATTAATTCGCTGC
TAAATAAAAATTATAGGGCTATATATGATGGGATCATATTCTATCTTTCTCTTTTTCAAA
TYCTTTATATTTTAGAATACAAATCGCTTTAAACAATAAAAAAGAGGTATATCCTACCCG
AATTGACACACACAATAAATAATAAATATATAAATAATAAAAATATAAATATATAAATTA
TAAATATATAAATTATATATATAAATATATTAACATGTGTGTAATTTGTTAAAATTGATA
TTAAACAGCTTTGCATGGGGAAAGAGTGGCTTTTATAAGCGAATCATATAATACAGTGTT
AATTTTAATATATAAAATATAATTTCCTTAAATTGTTATTTAACATTTTTAATTGCCTAC
CCARGGTAATATAATATCCAAATGACAATCAAAGATAAATGAAATACCGCTCCATTTTTC
CTATATATCCATTTTCATTTTGTGTAAATGTTCATAAAATTTTATCATATACTCATAAAA
AACAGGGGAAAAATGAAAAAATGAACGTCCACCCCGTGTGAATATGCTCATTTTGTATAC
TATACATTTTTTTTTATATAATTTATATTTTCCAAATAAAAAAAATGTTTGTACATGTAT
ATATCAATATTGTGTTATGGCATGATGAAAAACAATTATTTTATTCGTTTTTAAATTTGT
AGAATTTCCCTAAATTATTAATGATTTCATATTATAATATATGTATCTATATATTTTTAA
TGTGTAATTCAAAATGGCGAAAAAGAAACAAACCATACTTTATAAGCAACTCTCTTTGAG
CACTTACCATAAAAAATTATACTACTAAATTCAAATTAAATAAAAAAATATATATTATAA
AATTGGCTTTAATTATTTATAAGCACTTGCTTTGTTTTACTCAAAAAGTATTGGCATAAG
TATAAACATAAAACCAATTTTTCACAAAATATATTATATGTCCATACAACTATATCCGAA
CYTTCAAAACACATCCAAATTTCCATTTACCACATGCAAGCAAAAGGATATTAAATAAAA
AAAAATTATTTATATTTTCATGATCACTTAAATAATATTTAAAAAAAAAAGAGACCATAT
ATAAAATGTATAATTTAAATATAGCTAACTTATTAAAAAAAATGAATATTGAGCATATAT
CCATAATTTAAAACACTTCTATTTTATGTTATTTGATATATAAGTCGATAAATTATTGCA
TGTATGCATATAGACAGATTAATATACACCAAAATAATAAAAATTATATATAGTTCATAT
ATTATTCTGAATAATAAATTAGCTTTCCTCTTAATTCACTTGGTATGTATATATATATAT
ATATATATATATATATATATATGGATAAATAAATATAAGGTCTATAACAGGAAAAAATGT
GTATTAACTTTTTTTTATAATTATTTTATATTTTTTCTCCATTTATTTAAATATGTTAAA
ATTATTTCCCATTTTATAAACAAAAATTAATCCATAAAATAGCAAAATAACAAAATGACA
AAATAACAAAAACAAAAAAACGCATTATATGAGTTCATTTTACACAATCCAAAGAAAAAG
AAAATATGCTTAAAATATTCATAACACACTTTTAAGCTATAAAAATATATATATTTATTT
GTGTCTATATTACCAACTCAATTTAATAGATGTGTTATGTGATTAATTCATACACAAACA
TACAAAAATAAACACCATTTTTGAAAAGATTAATTTGAAATCATATATGTTATTATTATA
TATTTTTATATCAATGAAATTTAAAAAAATAAAAGGGAAATCAATGTATTAAAAATAATT
ATATGTTATTTTATTTCCACAATATTTATTTATTATTTATTGTTGACCTGCAGGCATGCA
AGCTTATCGATACCGTCGACCTCGAGGGGGGGCCCGGTACCCAGCTTTTGTTCCCTTTAG
TGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGT
TATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGT
GCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCG
GGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTG
CGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTG
CGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGAT
AACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCC
GCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGC
TCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGA
AGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTT
CTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTG
TAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGC
GCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTG
GCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTC
TTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTG
CTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACC
GCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCT
CAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGT
TAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAA
AAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAA
TGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCC
TGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCT
GCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCA
GCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATT
AATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTT
GCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCC
GGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGC
TCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTT
ATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACT
GGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGC
CCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATT
GGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCG
ATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCT
GGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAA
TGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGT
CTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGC
ACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGT
TAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCCTT
ATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTC
CACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATG
GCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCAC
TAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACG
TGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAG
CGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGT
CCCATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGC
TATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAG
GGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACTCAC
TATAGGGCGAATTGGAGCTCCACCGCGGTGGCGGCCGCTCTAGAACTAGTCGGTTTACTT
ACATTTTTATAACACATTATGTTTTACACATATAATTCTAAATATAATAGTAATATTATT
ATATAAATTTCAATAATAATCATAATTAAATAGCTAACATTTAAGGAGTCAGAACCTAAT
TAATGATATAGAGAATAAAACGCTCATTATTAAATTATAATTATTAATATATATTTATTA
GTATATAATAATATTTAAAAATCCGATAAATGCGTGAAATAAATTTAAAAATTATTTATA
TTTTTTAAATAAATATTAAGTTTTAGAATTTTTTAGAAAATCGTTTCCTTCAATTTAACA
CATTTATATACCTTTATACTGTTAATTTTCAATTCTAACAAATATATTAGCATTTTTTAA
TATTTTATTTATCAAATCCCAAAATGAATAAAGGATATATTAAGATTGCTTTGGCGCTTT
TAAGCGTAGCTGGATATATGCAAAATATGGCATTTGCAAGCGATTCTACCCCAAGCCCCA
ATTCGTAAGAAAATAATTGCAACATATATATCAAATATAAATATGTATATATGGTTTATT
ATAAGAATATTATATATATTTTGTGAGTGTATATATATATAACGCTTGGAAAATCAAAAT
AACTTCTTATAAATATATATTTTTGTTAATTAAAAATGCAAATAAATTTAAAAATATATT
GTTTTCTCTATATGTAGTCCAAATGAAACAAGTAAAGAAATATTAGATAACGACTCTGAA
GACGATATACAACAATCATCATCCAACCCTAAAGAAGTAGAACATCTATTATATGACGAC
CCTGAAGAAGCTATAAAAGCAGCAGATATTATGACTGAAGCTTTAGATTTTGCGCAAAAA
CATGCCGAGCATACAAATGATTACAAGGAATATTCTAAGCAAGATGGAGTAGTTTTATAT
TTTAAGAAATTCAAAGATACTGAAATTGGAAAACTTGAACTTACAATCCCAAACCCCGAT
AGTGTATGAATTAACAAGCAATAATTTTTAATTCATGAAATTATTTTAAAATAAAAAAAT
ATAATTATTAATATATAAATATGTTTTTTTTGTTATTAATAGTATGATGATGTAGTT
Restriction sites to linearize plasmid HpaI
Selectable marker used to select the mutant parasitetgdhfr
Promoter of the selectable markerpbdhfr
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modification
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1TACTAGTCGGTTTACTTACATTTTTATAACAC
Additional information primer 1L4656 (SpeI); PBANKA_132730 Forward
Sequence Primer 2TGGATCCCTTTTTAAAAATATCCTTTAATTTTACAAG
Additional information primer 2L4580 (BamHI); PBANKA_132730 Reverse
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6