SummaryRMgm-5587
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Successful modification | The parasite was generated by the genetic modification |
The mutant contains the following genetic modification(s) | Gene disruption |
Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 39037752 |
MR4 number | |
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Parent parasite used to introduce the genetic modification | |
Rodent Malaria Parasite | P. berghei |
Parent strain/line | P. berghei ANKA |
Name parent line/clone | Not applicable |
Other information parent line | |
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The mutant parasite was generated by | |
Name PI/Researcher | Ghosh A, Mishra S |
Name Group/Department | Division of Molecular Microbiology and Immunology |
Name Institute | CSIR-Central Drug Research Institute |
City | Lucknow |
Country | India |
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Name of the mutant parasite | |
RMgm number | RMgm-5587 |
Principal name | Scot1 KO |
Alternative name | |
Standardized name | |
Is the mutant parasite cloned after genetic modification | Yes |
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Phenotype | |
Asexual blood stage | Not different from wild type |
Gametocyte/Gamete | Not different from wild type |
Fertilization and ookinete | Not different from wild type |
Oocyst | Not different from wild type |
Sporozoite | Not different from wild type |
Liver stage | No blood stage infection in C57BL/6after after intravenous injection of salivary gland sporozoites or infected by mosquito bites. Normal invasion of hepatocytes. In livers of infected mice collected at 40 and 55 hpi, the parasite burden (quantified by amplifying 18S rRNA using real-time PCR), no difference in the 18S rRNA copy number was found at 40 hpi, but it was significantly lower at 55 hpi. Normal growth, numbers and sizes of liver stages. Reduced nuclear division at 62 hpi. No detached liver stages detected. |
Additional remarks phenotype | Mutant/mutation Analysis of a mutant expressing C-terminal 3xHA-mCherry-tagged version of Scot1 (RMgm-5589) showed expression in oocysts, sporozoites and liver stages (at 12, 24 and 62 hpi). Expression was not detected in the blood, gamete, ookinete, or liver stages (at 36 or 48 hpi). |
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Details of the target gene | |||||||||||||||||||||||||
Gene Model of Rodent Parasite | PBANKA_1411000 | ||||||||||||||||||||||||
Gene Model P. falciparum ortholog | PF3D7_1312500 | ||||||||||||||||||||||||
Gene product | conserved Plasmodium protein, unknown function | ||||||||||||||||||||||||
Gene product: Alternative name | Scot1; Sporozoite Conserved Orthologous Transcript 1 | ||||||||||||||||||||||||
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Details of the genetic modification | |||||||||||||||||||||||||
Inducable system used | No | ||||||||||||||||||||||||
Additional remarks inducable system | |||||||||||||||||||||||||
Type of plasmid/construct used | (Linear) plasmid double cross-over | ||||||||||||||||||||||||
PlasmoGEM (Sanger) construct/vector used | No | ||||||||||||||||||||||||
Modified PlasmoGEM construct/vector used | No | ||||||||||||||||||||||||
Plasmid/construct map | |||||||||||||||||||||||||
Plasmid/construct sequence | |||||||||||||||||||||||||
Restriction sites to linearize plasmid | |||||||||||||||||||||||||
Partial or complete disruption of the gene | Complete | ||||||||||||||||||||||||
Additional remarks partial/complete disruption | |||||||||||||||||||||||||
Selectable marker used to select the mutant parasite | hdhfr/yfcu | ||||||||||||||||||||||||
Promoter of the selectable marker | eef1a | ||||||||||||||||||||||||
Selection (positive) procedure | pyrimethamine | ||||||||||||||||||||||||
Selection (negative) procedure | No | ||||||||||||||||||||||||
Additional remarks genetic modification | To delete Scot1, two fragments, F3 (0.63 kb) and F4 (0.52 bp), were amplified using the primer sets 1172/1173 and 1167/1168 and cloned into SalI and NotI/AscI in the pBC-GFP-hDHFR:yFCU vector | ||||||||||||||||||||||||
Additional remarks selection procedure | |||||||||||||||||||||||||
Primer information: Primers used for amplification of the target sequences
![]() Primer information: Primers used for amplification of the target sequences
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