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Details of the target gene |
Gene Model of Rodent Parasite |
PBANKA_0605900
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Gene Model P. falciparum ortholog |
PF3D7_1207400
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Gene product | conserved Plasmodium protein, unknown function |
Gene product: Alternative name | S14 |
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Details of the genetic modification |
Name of the tag | 3xHA-mCherry |
Details of tagging | C-terminal |
Additional remarks: tagging | |
Commercial source of tag-antibodies | |
Type of plasmid/construct | (Linear) plasmid double cross-over |
PlasmoGEM (Sanger) construct/vector used | No |
Modified PlasmoGEM construct/vector used | No
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Plasmid/construct map |
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Plasmid/construct sequence |
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Restriction sites to linearize plasmid |
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Selectable marker used to select the mutant parasite | hdhfr |
Promoter of the selectable marker | eef1a |
Selection (positive) procedure | pyrimethamine |
Selection (negative) procedure | No |
Additional remarks genetic modification | For the endogenous tagging of S14 with 3XHA-mCherry, two fragments, F1 (0.74 kb) and F2 (0.65 kb), were amplified using 1010/1011 and 1005/1006 and cloned into plasmid pBC-3XHA-mCherry-hDHFR at XhoI/BglII and NotI/AscI, respectively. The plasmid was linearized using XhoI/AscI |
Additional remarks selection procedure | |
Primer information: Primers used for amplification of the target sequences
Primer information: Primers used for amplification of the target sequences
Sequence Primer 1 | |
Additional information primer 1 | |
Sequence Primer 2 | |
Additional information primer 2 | |
Sequence Primer 3 | |
Additional information primer 3 | |
Sequence Primer 4 | |
Additional information primer 4 | |
Sequence Primer 5 | |
Additional information primer 5 | |
Sequence Primer 6 | |
Additional information primer 6 | |
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