| Successful modification | The parasite was generated by the genetic modification |
| The mutant contains the following genetic modification(s) |
Gene tagging
|
| Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 37543672
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| MR4 number |
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| Parent parasite used to introduce the genetic modification |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | P. berghei ANKA |
| Name parent line/clone |
P. berghei ANKA 2.34
|
| Other information parent line | P. berghei ANKA 2.34 is a cloned, gametocyte producer line of the ANKA strain (PubMed: PMID: 15137943). |
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| The mutant parasite was generated by |
| Name PI/Researcher | Saeed S, Dessens JT |
| Name Group/Department | Department of Infection Biology, Faculty of Infectious and Tropical Diseases |
| Name Institute | London School of Hygiene & Tropical Medicine |
| City | London |
| Country | UK |
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| Name of the mutant parasite |
| RMgm number | RMgm-5398 |
| Principal name | FabG/GFP |
| Alternative name | |
| Standardized name | |
| Is the mutant parasite cloned after genetic modification | Yes |
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| Phenotype |
| Asexual blood stage | Not different from wild type |
| Gametocyte/Gamete | Not different from wild type |
| Fertilization and ookinete | Not different from wild type |
| Oocyst | No expression detected in blood stages, gametocytes and ookinetes. FabG expression was readily detected in young oocysts. Fluorescence was localised in a tubular branched structure reminiscent of the apicoplast, in agreement with localisation of FAS in this organelle. FabG::GFP remained apicoplast-localised throughout oocyst development and in sporulated oocysts located in discrete spots, likely refecting the division of the apicoplast tubular network into individual small apicoplasts during sporozoite budding. |
| Sporozoite | FabG::GFP remained apicoplast-localised throughout oocyst development and in sporulated oocysts located in discrete spots, likely refecting the division of the apicoplast tubular network into individual small apicoplasts during sporozoite budding. |
| Liver stage | Not tested |
| Additional remarks phenotype | Mutant/mutation
The mutant expresses a C-terminal GFP-tagged version of FabG
Protein (function)
FabG is an enzyme of the bacterial like type II fatty acid biosynthesis (FAS-II) pathway
Phenotype
No expression detected in blood stages, gametocytes and ookinetes. FabG expression was readily detected in young oocysts. Fluorescence was localised in a tubular branched structure reminiscent of the apicoplast, in agreement with localisation of FAS in this organelle. FabG::GFP remained apicoplast-localised throughout oocyst development and in sporulated oocysts located in discrete spots, likely refecting the division of the apicoplast tubular network into individual small apicoplasts during sporozoite budding.
Additional information
Other mutants |
*RMgm-5398
