SummaryRMgm-5273
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Successful modification | The parasite was generated by the genetic modification |
The mutant contains the following genetic modification(s) | Gene disruption |
Reference (PubMed-PMID number) | Not published (yet) |
MR4 number | |
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Parent parasite used to introduce the genetic modification | |
Rodent Malaria Parasite | P. yoelii |
Parent strain/line | P. y. yoelii 17XNL |
Name parent line/clone | Not applicable |
Other information parent line | |
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The mutant parasite was generated by | |
Name PI/Researcher | Goswami D, Vaughan AM |
Name Group/Department | Center for Global Infectious Disease Research |
Name Institute | Seattle Children’s Research Institute |
City | Seattle |
Country | USA |
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Name of the mutant parasite | |
RMgm number | RMgm-5273 |
Principal name | PY17X_1369800(-) |
Alternative name | |
Standardized name | |
Is the mutant parasite cloned after genetic modification | Yes |
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Phenotype | |
Asexual blood stage | Not different from wild type |
Gametocyte/Gamete | Not different from wild type |
Fertilization and ookinete | Not different from wild type |
Oocyst | Not different from wild type |
Sporozoite | Not different from wild type |
Liver stage | Not different from wild type |
Additional remarks phenotype | Mutant/mutation No details are provided in the manuscript on the generation of this gene-deletion mutant. From the manuscript: 'Gene knockout of PY17X_1369800 was achieved but the phenotype of the knockout was equivalent to wildtype across the life cycle and thus this gene deletion was not pursued further (data not shown). Published in: bioRxiv preprint doi: https://doi.org/10.1101/2022.12.13.519845 Protein (function) Phenotype No details are provided in the manuscript on the generation of this gene-deletion mutant. Additional information |
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Details of the target gene | |||||||||||||||||||||||||
Gene Model of Rodent Parasite | PY17X_1369800 | ||||||||||||||||||||||||
Gene Model P. falciparum ortholog | PF3D7_1351300 | ||||||||||||||||||||||||
Gene product | conserved Plasmodium membrane protein, unknown function | ||||||||||||||||||||||||
Gene product: Alternative name | |||||||||||||||||||||||||
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Details of the genetic modification | |||||||||||||||||||||||||
Inducable system used | No | ||||||||||||||||||||||||
Additional remarks inducable system | |||||||||||||||||||||||||
Type of plasmid/construct used | unknown | ||||||||||||||||||||||||
PlasmoGEM (Sanger) construct/vector used | No | ||||||||||||||||||||||||
Modified PlasmoGEM construct/vector used | No | ||||||||||||||||||||||||
Plasmid/construct map | |||||||||||||||||||||||||
Plasmid/construct sequence | |||||||||||||||||||||||||
Restriction sites to linearize plasmid | |||||||||||||||||||||||||
Partial or complete disruption of the gene | Complete | ||||||||||||||||||||||||
Additional remarks partial/complete disruption | |||||||||||||||||||||||||
Selectable marker used to select the mutant parasite | unknown | ||||||||||||||||||||||||
Promoter of the selectable marker | unknown | ||||||||||||||||||||||||
Selection (positive) procedure | N.A | ||||||||||||||||||||||||
Selection (negative) procedure | N.A. | ||||||||||||||||||||||||
Additional remarks genetic modification | No details are provided in the manuscript on the generation of this gene-deletion mutant. From the manuscript: 'Gene knockout of PY17X_1369800 was achieved but the phenotype of the knockout was equivalent to wildtype across the life cycle and thus this gene deletion was not pursued further (data not shown). | ||||||||||||||||||||||||
Additional remarks selection procedure | |||||||||||||||||||||||||
Primer information: Primers used for amplification of the target sequences
Primer information: Primers used for amplification of the target sequences
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