| Successful modification | The parasite was generated by the genetic modification |
| The mutant contains the following genetic modification(s) |
Gene tagging
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| Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 35301756
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| MR4 number |
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| Parent parasite used to introduce the genetic modification |
| Rodent Malaria Parasite | P. berghei |
| Parent strain/line | P. berghei ANKA |
| Name parent line/clone |
Not applicable
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| Other information parent line | |
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| The mutant parasite was generated by |
| Name PI/Researcher | Singh D, Kumar KA |
| Name Group/Department | Department of Animal Biology, School of Life Sciences |
| Name Institute | University of Hyderabad |
| City | Hyderabad |
| Country | India |
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| Name of the mutant parasite |
| RMgm number | RMgm-5195 |
| Principal name | PbSIMP 3XHA |
| Alternative name | |
| Standardized name | |
| Is the mutant parasite cloned after genetic modification | Yes |
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| Phenotype |
| Asexual blood stage | Not tested |
| Gametocyte/Gamete | Immunofluorescence assay (IFA) revealed HA expression in gametocytes, ookinetes, oocyst and salivary gland sporozoites. The HA immunoreactivity was majorly localised to gametocyte cytoplasm; however, in ookinetes, oocyst and salivary gland sporozoites, a peripheral/membrane localisation was observed. |
| Fertilization and ookinete | Immunofluorescence assay (IFA) revealed HA expression in gametocytes, ookinetes, oocyst and salivary gland sporozoites. The HA immunoreactivity was majorly localised to gametocyte cytoplasm; however, in ookinetes, oocyst and salivary gland sporozoites, a peripheral/membrane localisation was observed. |
| Oocyst | Immunofluorescence assay (IFA) revealed HA expression in gametocytes, ookinetes, oocyst and salivary gland sporozoites. The HA immunoreactivity was majorly localised to gametocyte cytoplasm; however, in ookinetes, oocyst and salivary gland sporozoites, a peripheral/membrane localisation was observed. |
| Sporozoite | Immunofluorescence assay (IFA) revealed HA expression in gametocytes, ookinetes, oocyst and salivary gland sporozoites. The HA immunoreactivity was majorly localised to gametocyte cytoplasm; however, in ookinetes, oocyst and salivary gland sporozoites, a peripheral/membrane localisation was observed. |
| Liver stage | Not tested |
| Additional remarks phenotype | Mutant/mutation
The mutant expresses a C-terminal 3xHA-tagged version of PIMMS22/SIMP
Protein (function)
PfPIMMS22 encodes a 393 amino acid (45 kDa) protein and PbPIMMS22/SIMP encodes a 393 aa (44 kDa) protein, both with no predicted signal peptide or transmembrane domain. The protein is highly conserved amongst Plasmodium orthologues. PyPIMMS22 (PY17X_1424900) was previously identified in salivary gland sporozoites through a subtractive hybridization (SSH) profiling and termed sporozoite protein S15. The same protein was also identified in midgut oocyst sporozoites as an interacting partner to the apicomplexan specific RNA-binding protein, ALBA4, which is involved in mRNA regulation in gametocyte and midgut oocyst sporozoite development. PIMMS22 homologues are found in other apicomplexan parasites including Toxoplasma gondii, Neospora caninum and Eimeria with sequence identities to PIMMS22 ranging from 36% to 42%. InterPro domain analysis revealed no recognizable domain in PIMMS22.
Phenotype
Immunofluorescence assay (IFA) revealed HA expression in gametocytes, ookinetes, oocyst and salivary gland sporozoites. The HA immunoreactivity was majorly localised to gametocyte cytoplasm; however, in ookinetes, oocyst and salivary gland sporozoites, a peripheral/membrane localisation was observed.
See also mutants RMgm-4999, RMgm-5127, RMgm-5194 lacking expression of SIMP (PIMS22/concavin) with (slightly) different phenotypes
Additional information
Other mutants
See also mutants RMgm-4999, RMgm-5127, RMgm-5194 lacking expression of SIMP (PIMS22/concavin) with (slightly) different phenotypes
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*RMgm-5195
