Mutant/mutation
In the mutant the P. berghei concavin (PIMMS22) gene has been replaced with the P. falciparum Concavin (PIMMS22) gene (PF3D7_0814600). 

Protein (function)
See mutant RMgm-4999 for previous analyses of this protein (PIMMS22; Concavin):
PfPIMMS22 encodes a 393 amino acid (45 kDa) protein and PbPIMMS22 encodes a 393 aa (44 kDa) protein, both with no predicted signal peptide or transmembrane domain. The protein is highly conserved amongst Plasmodium orthologues. PyPIMMS22 (PY17X_1424900) was previously identified in salivary gland sporozoites through a  subtractive hybridization (SSH) profiling and termed sporozoite protein S15. The same protein was also identified in midgut oocyst sporozoites as an interacting partner to the apicomplexan specific RNA-binding protein, ALBA4, which is involved in mRNA regulation in gametocyte and midgut oocyst sporozoite development. PIMMS22 homologues are found in other apicomplexan parasites including Toxoplasma gondii, Neospora caninum and Eimeria with sequence identities to PIMMS22 ranging from 36% to 42%. InterPro domain analysis revealed no recognizable domain in PIMMS22. Evidence is presented for ookinete defects in invasion and traversal of the midgut epithelium (although this cannot completely explain the strongly reduced oocysts numbers, indicating reduced ookinete to oocyst transition after midgut traversal). Strongly reduced oocyst numbers. Strongly reduced sporozoite numbers. No infection of mice by the bite of infected mosquitoes.

From this study: Due to its impact on the convex-concave polarity of sporozoites we named the protein Concavin

Phenotype
No phenotypes of oocysts and sporozoites different from wild type were detected, indicating that P. falciparum concavin complements the function of P. berghei concavin

Additional information

Other mutants