RMgmDB - Rodent Malaria genetically modified Parasites


Malaria parasiteP. berghei
DisruptedGene model (rodent): PBANKA_0506600; Gene model (P.falciparum): PF3D7_1022400; Gene product: serine/arginine-rich splicing factor 4 (nucleolar protein 3 (NPL3))
Phenotype Asexual bloodstage; Gametocyte/Gamete;
Last modified: 26 October 2021, 15:20
Successful modificationThe parasite was generated by the genetic modification
The mutant contains the following genetic modification(s) Gene disruption
Reference (PubMed-PMID number) Reference 1 (PMID number) : 34604117
MR4 number
Parent parasite used to introduce the genetic modification
Rodent Malaria ParasiteP. berghei
Parent strain/lineP. berghei ANKA
Name parent line/clone Not applicable
Other information parent line
The mutant parasite was generated by
Name PI/ResearcherNiikura M, Kobayashi F
Name Group/DepartmentDepartment of Environmental Science, School of Life and Environmental Science
Name InstituteAzabu University
Name of the mutant parasite
RMgm numberRMgm-5088
Principal nameΔnpl3
Alternative name
Standardized name
Is the mutant parasite cloned after genetic modificationYes
Asexual blood stageNormal (wild type) growth of asexual blood stages.
Gametocyte/GameteNormal (wild type) gametocyte production.
Fertilization and ookineteNot tested
OocystNot tested
SporozoiteNot tested
Liver stageNot tested
Additional remarks phenotype

The mutant lacks expression of NPL3

Protein (function)
Nuclear poly(A) binding protein 2 (NAB2), THO complex subunit 4 (THO4), nucleolar protein 3 (NPL3), G-strand binding protein 2 (GBP2) and serine/arginine-rich splicing factor 1 (SR1) are involved in nuclear mRNA export in malaria parasites.
In S. cerevisiae, individual serine/arginine-rich (SR) proteins proteins such as NPL3, GBP2, and HRB1 are not essential. NPL3, GBP2 and SR1 contain conserved RNA recognition motifs.

Normal (wild type) growth of asexual blood stages. Normal (wild type) gametocyte production.

Additional information

Other mutants

  Disrupted: Mutant parasite with a disrupted gene
Details of the target gene
Gene Model of Rodent Parasite PBANKA_0506600
Gene Model P. falciparum ortholog PF3D7_1022400
Gene productserine/arginine-rich splicing factor 4
Gene product: Alternative namenucleolar protein 3 (NPL3)
Details of the genetic modification
Inducable system usedNo
Additional remarks inducable system
Type of plasmid/construct used(Linear) plasmid double cross-over
PlasmoGEM (Sanger) construct/vector usedNo
Modified PlasmoGEM construct/vector usedNo
Plasmid/construct map
Plasmid/construct sequence
Restriction sites to linearize plasmid
Partial or complete disruption of the geneComplete
Additional remarks partial/complete disruption
Selectable marker used to select the mutant parasitehdhfr
Promoter of the selectable markereef1a
Selection (positive) procedurepyrimethamine
Selection (negative) procedureNo
Additional remarks genetic modificationTo generated nab2-, tho4-, npl3- and sr1-deleted P. berghei ANKA, the gene-targeting vectors for nab2 (PBANKA_1122000), tho4 (PBANKA_1230500), npl3 (PBANKA_0506600) and sr1 (PBANKA_1232100) were prepared by PCR. Briefly, the 5’ and 3’ flanking regions of the open reading frame (ORF) of target genes were amplified by PCR. The PCR products were annealed to either side of the human dihydrofolate reductase (hdhfr)-expressing cassette and amplified by PCR using gene-specific primers The gene-targeting vectors were introduced into the ORFs of target genes by double-crossover homologous recombination
Additional remarks selection procedure
Primer information: Primers used for amplification of the target sequences  Click to view information
Primer information: Primers used for amplification of the target sequences  Click to hide information
Sequence Primer 1
Additional information primer 1
Sequence Primer 2
Additional information primer 2
Sequence Primer 3
Additional information primer 3
Sequence Primer 4
Additional information primer 4
Sequence Primer 5
Additional information primer 5
Sequence Primer 6
Additional information primer 6