Successful modification | The parasite was generated by the genetic modification |
The mutant contains the following genetic modification(s) |
Gene tagging,
Introduction of a transgene,
Introduction of a transgene
|
Reference (PubMed-PMID number) |
Reference 1 (PMID number) : 35994647 |
MR4 number |
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Parent parasite used to introduce the genetic modification |
Rodent Malaria Parasite | P. berghei |
Parent strain/line | P. berghei ANKA |
Name parent line/clone |
RMgm-1320
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Other information parent line | This transgenic reporter line (1868cl1; RMgm-1320) expresses luciferase under the control of the eef1α (PBANKA_1133300) promoter and, in addition, mCherry under the control of the hsp70 (PBANKA_0711900) promoter. Both reporter cassettes are introduced into the silent 230p locus, using a single DNA construct. This transgenic line does not contain a drug-selectable marker |
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The mutant parasite was generated by |
Name PI/Researcher | Kolli SK, Janse CJ |
Name Group/Department | Malaria Research Group, Department of Parasitology, |
Name Institute | Leiden University medical Center (LUMC) |
City | Leiden |
Country | The Netherlands |
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Name of the mutant parasite |
RMgm number | RMgm-5059 |
Principal name | 3272 |
Alternative name | Pbqc::cmyc |
Standardized name | |
Is the mutant parasite cloned after genetic modification | No |
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Phenotype |
Asexual blood stage | Not different from wild type |
Gametocyte/Gamete | QC expression in (female) gametocytes |
Fertilization and ookinete | QC expression in (female) gametocytes and ookinetes |
Oocyst | Not tested |
Sporozoite | QC expression in sporozoites |
Liver stage | Not different from wild type |
Additional remarks phenotype | Mutant/mutation
The mutant expresses a C-terminal cmyc-tagged version of QC and expresses mCherry and luciferase under control of constitutive promoters.
Protein (function)
N-terminal modification of glutamine or glutamic acid residues to pyroglutamic acid (pGlu; 5-oxo-L-46 proline) is a posttranslational modification (PTM), catalyzed by glutaminyl cyclases (QCs) found in eukaryotes and prokaryotes. Two evolutionary unrelated classes exist; mammalian QCs and QCs of bacteria, plants and parasites, which share no sequence homology, supporting a different evolutionary origin. Mammalian cells can express two forms, the secreted glutaminylâpeptide cyclotransferase (QPCT) or its iso-enzyme (QPCTL), localized in the Golgi complex. pGlu is implicated in maturation and stabilization of mammalian proteins such has neuropeptides and cytokines. QC activity has been associated in humans with pathological processes such as amyloidotic diseases and QPCTL is critical for pGLu formation on CD47, facilitating myeloid immune evasion.
A single gene encoding a glutaminyl cyclase (QC), named glutaminyl-peptide cyclotransferase, has been identified by electronic annotation in all sequenced Plasmodium genomes. Plasmodium QCs share 70-76% sequence similarity and 50-54% identity and contain a transmembrane domain. QC of the human malaria parasite P. falciparum (PfQC) shows 21-27% identity to QCs of various 64 bacteria and the plant Carica papaya (CpQC). All 9 amino acids of the catalytic site of bacterial and plant QCs are conserved in Plasmodium QC.
Phenotype
IFA analyses using cmyc antibodies showed QC::cmyc expression in (female) gametocytes, ookinetes and sporozoites
Additional information
From the Abstract:
'We show that Plasmodium sporozoites of QC-null mutants are recognized by the mosquito immune system and melanized when they reach the hemocoel. Sporozoite numbers in salivary glands are also reduced in mosquitoes infected with QC-null or QC catalytically-dead mutants. This phenotype can be rescued by genetic complementation or by disrupting mosquito hemocytes or melanization immune responses. Mutation of a single QC-target glutamine of the major sporozoite surface protein (CSP) also results in immune recognition of sporozoites. These findings reveal QC-mediated post-translational modification of surface proteins as a major mechanism of mosquito immune evasion by Plasmodium sporozoites'.
Other mutants |